miR‐541 suppresses proliferation and invasion of squamous cell lung carcinoma cell lines via directly targeting high‐mobility group <scp>AT</scp>‐hook 2

Xu, Li; Du, Bin; Lu, Qijue; Fan, Xiaowen; Tang, Ke; Yang, Lie; Liao, Wei-Lin

doi:10.1002/cam4.1491

Cited by 20 publications

(17 citation statements)

References 32 publications

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“…The important roles of HMGA2 and its downstream genes in tumor progression have been demonstrated in earlier studies 4,5 . Our previous studies have also identified HMGA2 as an oncogene in squamous cell lung carcinoma 6,20 . In this study, using RNA sequencing, we identified specific expression patterns of HMGA2-related circRNAs in the human lung adenocarcinoma cell line A549.…”

Section: Discussionmentioning

confidence: 84%

“…The important roles of HMGA2 and its downstream genes in tumor progression have been demonstrated in previous studies 4,5 . One of our previous studies showed that HMGA2 enhanced cell proliferation and invasion in squamous cell lung cancer 6 . To date, the regulatory effects of HMGA2 on protein-coding oncogenes have been demonstrated by multiple studies.…”

Section: Introductionmentioning

confidence: 94%

“…The migration assay was performed as described elsewhere 6 . Images were taken at 0 and 36 h. The migration distance was calculated as the difference in the gap width between the two imaging time points.…”

Section: Migration and Invasion Assaysmentioning

confidence: 99%

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HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma

Zhang³

et al. 2020

Cell Death Dis

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In this study, we identified a circular form of ASPH RNA (circASPH), expression of which was upregulated in lung adenocarcinoma and the human lung adenocarcinoma cell lines. We also found a positive correlation between circASPH level and the T and N stages of lung adenocarcinoma patients. Patients with higher levels of circASPH had a shorter overall survival. Moreover, we demonstrated that circASPH was directly regulated by HMGA2 and Twist1. The direct positive regulation of circASPH by Twist1 was dependent on the presence of HMGA2. Functional assays indicated that circASPH promoted the proliferation, migration, and invasion of lung adenocarcinoma cell lines in vitro. The promoting effect of tumor growth by circASPH was also observed in vivo. Mechanistically, circASPH was identified to act as a molecular sponge for miR-370 and abrogate miR-370-mediated inhibition of HMGA2. Finally, we demonstrated that the oncogenic function of circASPH was HMGA2-dependent. These findings reveal the oncogenic functions of the HMGA2-circASPH-HMGA2 axis and may be useful in developing circRNA-based therapeutic strategies for lung adenocarcinoma.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 94%

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HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma

Zhang³

et al. 2020

Cell Death Dis

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“…Upregulation of eca‐miR‐493b and eca‐miR‐541 was associated with advanced tumour stage (ESA) in ES tumours and might therefore also be of prognostic significance. Interestingly, these miRNAs possess rather tumour‐suppressive functions in diverse human cancers where they are often downregulated . If upregulation of eca‐miR‐493b and eca‐miR‐541 drives oncogenesis in ES and whether inhibition of these two miRNAs could possibly stop ES disease progression, needs to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed microRNAs, including a large microRNA cluster on chromosome 24, are associated with equine sarcoid and squamous cell carcinoma

Bogedale

Jagannathan

Gerber

et al. 2019

Vet Comparative Oncology

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The aim of this study was to investigate microRNA (miRNA) differential expression in the two most common equine skin tumours, equine sarcoid (ES) and squamous cell carcinoma (SCC), and its potential influence on the tumour microenvironment at post‐transcriptional level. We investigated miRNA fingerprints in four subgroups: mild (ESM) and aggressive (ESA) ES and ocular SCC (oSCC) and genital SCC (gSCC). Three tumours and three control samples were included in each of the four subgroups. Following next generation sequencing, miRNA differential expression analysis using DESeq2 was carried out. Pathways associated with the human mature homologues of identified dysregulated miRNAs were predicted using DIANA‐ miRPath v3.0. When comparing tumour vs control tissue, 57 miRNAs in ESM, six in ESA, 47 in oSCC and zero in gSCC were found to be differentially expressed and may thus serve as potential diagnostic tissue biomarkers. Whereas, ES lesions in general were associated with downregulation of the miR‐200 family, which may trigger epithelial‐mesenchymal transition, ESM lesions were associated with upregulation of the proposed tumour‐suppressive miRNA cluster on equine chromosome 24. In contrast, the oSCC tumours showed downregulation of this cluster as well as downregulation of the miR‐34 family, which may favour oSCC tumour cell metabolism. To further validate the proposed diagnostic miRNA fingerprints and their suggested biological effects, further miRNA studies need to be carried out in larger study cohorts.

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“…MiR-541 acts as a tumor suppressor in some cancers including lung cancer and pancreatic cancer. Overexpression of miR-541 has been reported to reverse cancer progression through suppression proliferation and invasion of cancer cells [34][35][36]. We discovered that recovery of miR-541 expression in gemcitabine-resistant PCa cells resensitized them to gemcitabine treatment.…”

Section: Discussionmentioning

confidence: 99%

Restore of miR-541 resensitizes pancreatic cancer cells to gemcitabine-induced apoptosis through suppression of HAX-1

Hong

Gan²,

Zhang

et al. 2020

Preprint

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Background: MiR-541 acts as a tumor suppressor in some cancers. However, the role of miR-541 in regulating the chemosensitivity to cancer cells is still unclear. The aim of this study is to explore the effect of miR-541 on chemoresistance of pancreatic cancer (PCa) cells to gemcitabine-induced apoptosis.Methods: Gemcitabine-resistant Panc-1 and Capan-2 PCa cell lines (Panc-1/R and Capan-2/R) were established through long term exposure to gemcitabine. Effect of miR-541 on changing the sensitivity of Panc-1/R and Capan-2/R to gemcitabine-induced cytotoxicity was evaluated by MTT assays. Regulation of miR-541 on HAX-1 was confirmed by bioinformatics, western blot analysis and luciferase reporter assays. Cell apoptosis and mitochondrial membrane potential (MMP) was measured by flow cytometry analysis.Results: Comparison with Panc-1 and Capan-2, downregulation of miR-541 was observed in Panc-1/R and Capan-2/R cells. Overexpression of miR-541 was found to increase the cytotoxicity of gemcitabine to Panc-1/R and Capan-2/R cells. However, transfection with HAX-1 plasmid can abolish the effect of miR-541 on gemcitabine-induced cytotoxicity against Panc-1/R and Capan-2/R.Conclusion: Downregulation of miR-541 is responsible for development of gemcitabine resistance in PCa. Overexpression of miR-541 may represent a potential strategy to reverse the chemoresistance of PCa.

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miR‐541 suppresses proliferation and invasion of squamous cell lung carcinoma cell lines via directly targeting high‐mobility group AT‐hook 2

Cited by 20 publications

References 32 publications

HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma

HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma

Differentially expressed microRNAs, including a large microRNA cluster on chromosome 24, are associated with equine sarcoid and squamous cell carcinoma

Restore of miR-541 resensitizes pancreatic cancer cells to gemcitabine-induced apoptosis through suppression of HAX-1

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