miR-5590-3p inhibited tumor growth in gastric cancer by targeting DDX5/AKT/m-TOR pathway

Wu, Nan; Han, Yong Chol; Liu, Haiming; Jiang, Mingzuo; Chu, Yi; Cao, Jiasheng; Lin, Jia; Liu, Yuanning; Xu, Bing; Xie, Xin

doi:10.1016/j.bbrc.2018.07.068

Cited by 34 publications

(36 citation statements)

References 21 publications

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“…Results suggested that miR‐5590‐3p had the strongest interaction with SOX9‐AS1. Previously, a study showed that miR‐5590‐3p was a tumor‐suppressor gene in gastric cancer (Wu et al ., ). In our study, we initially uncovered the downregulation of miR‐5590‐3p in HCC and confirmed that SOX9‐AS1 regulated SOX9/Wnt/β‐catenin through miR‐5590‐3p.…”

Section: Discussionmentioning

confidence: 97%

“…The miRNA are conserved non‐coding transcripts with about 22 nucleotides, responsible for silencing gene expression through targeting 3′UTR of mRNA (Bartel, ; Brümmer and Hausser, ). The miR‐5590‐3p is a newly identified miRNA exhibiting inhibitive function in gastric cancer through targeting DDX5/AKT/mTOR pathway (Wu et al ., ), but its involvement in HCC and correlation with SOX9‐AS1 and SOX9 have rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

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A SOX9‐AS1/miR‐5590‐3p/SOX9 positive feedback loop drives tumor growth and metastasis in hepatocellular carcinoma through the Wnt/β‐catenin pathway

Zhang

Hou

et al. 2019

Molecular Oncology

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Hepatocellular carcinoma (HCC) is a prevalent solid tumor with a high global death rate. SRY box 9 (SOX9) has been reported as an oncogene in HCC by several studies, but the underlying mechanism remains largely unexplored. Here, we confirmed upregulation of SOX9 in HCC tissues and cell lines and validated that SOX9 facilitates proliferation, migration and invasion in HCC. We subsequently identified that the long non‐coding RNA (lncRNA) SOX9 antisense RNA 1 (SOX9‐AS1) is a neighbor gene to SOX9; SOX9‐AS1 is also upregulated in HCC, and its expression is positively correlated with that of SOX9. In addition, SOX9‐AS1 appears to have prognostic significance in HCC patients. We showed that SOX9‐AS1 aggravates HCC progression and metastasis in vitro and in vivo. We demonstrated that SOX9‐AS1 sponges miR‐5590‐3p to elevate SOX9 expression, and that SOX9 in turn transcriptionally activates SOX9‐AS1. Moreover, we verified that SOX9‐AS1 regulates SOX9 and its known downstream Wnt/β‐catenin pathway so as to facilitate epithelial‐to‐mesenchymal transition. The results of our rescue assays suggest that SOX9‐AS1 regulates HCC progression through SOX9 and the Wnt/β‐catenin pathway. In conclusion, our study demonstrates that a SOX9‐AS1/miR‐5590‐3p/SOX9 positive feedback loop drives tumor growth and metastasis in HCC through the Wnt/β‐catenin pathway, suggesting SOX9‐AS1 as a novel potential prognostic and treatment target for HCC.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

A SOX9‐AS1/miR‐5590‐3p/SOX9 positive feedback loop drives tumor growth and metastasis in hepatocellular carcinoma through the Wnt/β‐catenin pathway

Zhang

Hou

et al. 2019

Molecular Oncology

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“…Human CRC cell lines HCT‐116, LoVo, SW‐480, SW‐620, HT‐29 and immortalized colon epithelial cell line FHC were purchased from Cell bank of Chinese Academy of Sciences. All the cells were cultured in a DMEM medium (Gibco, USA) supplemented with 10% foetal bovine serum (Gibco) and 100 U/mL penicillin/streptomycin at 37°C in a humidified incubator containing 5% CO 2 …”

Section: Methodsmentioning

confidence: 99%

CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR‐377 to induce E2F3 expression

Yang

et al. 2020

J Cellular Molecular Medi

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CircPRTM5 is associated with cell proliferation and migration in many kinds of malignancies. However, the functions and mechanisms of CircPRTM5 in CRC progression remain unclear. We explored the role and the mechanisms of CircPRTM5 in the development of CRC. Tissues of CRC patients and matched adjacent non‐tumour tissues were collected to evaluate the expression of CircPRTM5. The expression of CircPRTM5 in CRC tissues was significantly higher than that in adjacent tissues. The biological functions of CircPRTM5 in CRC were determined by overexpression and down‐regulation of CircPRTM5 in CRC cells in vitro and in vivo. The results indicate that knockdown of CircPRTM5 can significantly inhibit the proliferation of CRC cells. The potential mechanisms of CircPRTM5 in CRC development were identified by RT‐qPCR, Western blotting analysis and luciferase reporter assay. CircPRTM5 competitively regulates the expression of E2F3 by capillary adsorption of miR‐377. CircPRMT5 regulates CRC proliferation by regulating the expression of E2F3, which affects the expression of the cell cycle‐associated proteins cyclinD1 and CDK2. CircPRTM5 exerts critical regulatory role in CRC progression by sponging miR‐377 to induce E2F3 expression.

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“…These ncRNA molecules over 200 nucleotides in length are named long non‐coding RNAs (lncRNAs) that regulate mRNA translation, transcriptional processes and cell development, differentiation, proliferation and apoptosis . There is increasing evidence that lncRNA can be used as a biomarker for the diagnosis and prognosis of various cancers, such as colorectal cancer, breast cancer, breast cancer, liver cancer and gastric cancer . In addition, in the study of liver metastases from colorectal cancer, it was found that lncRNA UICLM inhibits the expression of miR‐150‐5p by competitive endogenous RNA, thereby promoting liver metastasis of colorectal cancer .…”

Section: Introductionmentioning

confidence: 99%

LINC01303 functions as a competing endogenous RNA to regulate EZH2 expression by sponging miR‐101‐3p in gastric cancer

Cao

et al. 2019

J Cellular Molecular Medi

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Long non‐coding RNA (lncRNA) is one of the important regulators of many malignancies. However, the biological function and clinical significance of a large number of lncRNAs in gastric cancer remain unclear. Therefore, we analysed the TCGA data to find that LINC01303 is significantly up‐regulated in gastric cancer tissues. However, the biological function of LINC01303 in GC remains unknown. In our study, we found that the expression of LINC01303 was significantly higher in GC tissues than in adjacent tissues by real‐time quantitative PCR. We can significantly inhibit the malignant proliferation, migration and invasion of GC cells by silencing LINC01303 expression. In addition, LINC01303 knockdown can also inhibit GC growth in vivo. After the bioinformatics analysis, we found that LINC01303 can be used as a miR‐101‐3p sponge to competitively adsorb miR‐101‐3p with EZH2. Therefore, our results indicate that LINC01303 promotes the expression of EZH2 by inhibiting miR‐101‐3p activity and promotes GC progression. In summary, in this study, we demonstrated for the first time that the LINC01303/miR‐101‐3p/EZH2 axis promotes GC progression.

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miR-5590-3p inhibited tumor growth in gastric cancer by targeting DDX5/AKT/m-TOR pathway

Cited by 34 publications

References 21 publications

A SOX9‐AS1/miR‐5590‐3p/SOX9 positive feedback loop drives tumor growth and metastasis in hepatocellular carcinoma through the Wnt/β‐catenin pathway

A SOX9‐AS1/miR‐5590‐3p/SOX9 positive feedback loop drives tumor growth and metastasis in hepatocellular carcinoma through the Wnt/β‐catenin pathway

CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR‐377 to induce E2F3 expression

LINC01303 functions as a competing endogenous RNA to regulate EZH2 expression by sponging miR‐101‐3p in gastric cancer

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