MiR-590-3p promotes proliferation and metastasis of colorectal cancer via Hippo pathway

Abstract

Studies reported that miR-590-3p was involved in human cancer progression. However, its roles of oncogene or anti-oncogene in malignancies still remain elusive. This study was aimed to investigate the effect of miR-590-3p on the cell proliferation and metastasis via Hippo pathway in colorectal cancer (CRC). In our study, miR-590-3p was demonstrated highly expressed in CRC tissues, compared with adjacent normal tissues (P<0.05). In addition, miR-590-3p was positively associated with TNM stage and distant metastasis. Survival analysis showed that high miR-590-3p was related with poor overall survival rate. Then, over-expressed miR-590-3p was demonstrated to promote proliferation, invasion and migration of colon caner cells. What’s more, MST1, LATS1 and SAV1 mRNA were showed lowly expressed and YAP1 expression in mRNA and protein levels were highly expressed in CRC tissues, compared with adjacent normal tissues (all P<0.05). miR-590-3p expression was negatively associated with LATS1 and SAV1 mRNA respectively and positively related with YAP1 mRNA in CRC tissues, meanwhile, there was no relationship between miR-590-3p and MST1 mRNA. Furthermore, over-expressing miR-590-3p inhibited expressions of LATS1 and SAV1, promoted YAP1 expression and didn’t effect MST1 expression in colon cancer cells. And luciferase assay showed that miR-590-3p over-expression inhibited the luciferase activity of LATS1 and SAV1 3’UTR, meanwhile it had no effect on the mutated form of these two plasmids. Taken together, these data suggest that highly-expressed miR-590-3p promotes biological effect of proliferation and metastasis via targeting Hippo pathway, and predicts worse clinical outcomes of CRC patients.

Loss of PPM1F expression predicts tumour recurrence and is negatively regulated by miR‐590‐3p in gastric cancer

“…Multivariate analysis also revealed miR‐590 as an independent prognostic factor for tumour recurrence of GC patients. Intriguingly, our findings for the functions of miR‐590 in GC were also supported by some other studies in a variety of cancers, such as colorectal cancer (CRC), cervical cancer and glioblastoma, but opposed by those in CRC and glioblastoma multiforme . Thus, our above results showed that miR‐590 might act as an oncogenic marker in GC by targeting PPM1F.…”

miR-590-3p Promotes Ovarian Cancer Growth and Metastasis via a Novel FOXA2–Versican Pathway

“…Although the role of miR-590-3p in EOC has not previously been established, several studies have reported both tumorpromoting and tumor-suppressive effects of miR-590-3p in other types of cancer. For example, miR-590-3p promotes cell proliferation and invasion in T-cell acute lymphoblastic leukemia by inhibiting RB1 (6) and in colorectal cancer by targeting the Hippo pathway (7) and by promoting b-catenin signaling (32). Similarly, tumor-promoting effects of miR-590-3p were observed in glioblastoma (33).…”

“…miR-590-3p was first reported to enhance cardiomyocyte proliferation and cardiac regeneration after myocardial infarction (5). More recently, the effects of miR-590-3p on several types of cancer have been reported (6)(7)(8). Although the role of miR-590-3p in ovarian cancer is unknown, an RNA sequencing (RNA-seq) study has identified miR-590-3p as one of the miRNAs dysregulated in EOC tumors (9), suggesting its potential role in EOC development.…”

Contributions of Gene Modules Regulated by Essential Noncoding RNA in Colon Adenocarcinoma Progression

“…Overexpressed miR-362-3p influences cell cycle arrest and reduces cell viability and proliferation in CRC cells, which is associated with the recurrence of CRC [42]. miR-590-3p accelerates cellular proliferation and metastasis via targeting the Hippo pathway, and it can predict worse clinical outcomes of CRC patients [43].…”