MiR-630 Regulates Proliferation and Apoptosis of Clear Cell Renal Cell Carcinoma Through Targeted Binding to Phosphatase and Tensin Homolog

Abstract

Clear cell renal cell carcinoma (ccRCC) occurs as tumor cells in the kidney quickly accumulate and forms a lump (mass). Previous evidence showed that miR-630 overexpression is associated with gastric cancer progression. In this study, the differentially expressed miRNAs were screened in ccRCC patients using the gene expression profile chip. The possible targets for miR-630 were predicted by Target Scan and assessed via the luciferase reporter gene assay. The cell proliferation and apoptosis were detected via CCK-8 assay and flow cytometry. The Bcl-2, Bax, PTEN as well as downstream PI3K/AKT/mTOR signaling were detected via Western blotting. The result revealed that in silico analysis using the Target Scan software predicted that PTEN might be a potential target for miR-630, which was further verified via the luciferase reporter gene assay. The increase of miR-630 expression significantly raised the proliferation rate (P < 0.05) and reduced cellapoptosis (P < 0.05). In addition, the results of Western blotting also revealed that miR-630 mimic could activate PI3K/AKT/mTOR signaling (P < 0.05). In conclusion, miR-630 cantargeted reduce the PTEN expression, and then activate the PI3K/AKT/mTOR signaling, ultimately promoting cell proliferation and inhibiting apoptosis of ccRCC.

The relationship between microRNAs and Rab family GTPases in human cancers

“…miR‐448, as a RAB2B inhibitor, induced cell cycle arrest and promoted cell apoptosis in PC (Jin, Wu, Zhou, Sun, & Wang, ). RCC is another common cancer with a poor prognosis, in which has also been reported the association between miRNAs and RAB proteins (Chen et al, ; Zhao et al, ). miR‐let‐7d is a tumor suppressor miRNA found in various types of cancer.…”