miR-652 Promotes Tumor Proliferation and Metastasis by Targeting RORA in Endometrial Cancer

Abstract

: Endometrial cancer is the most common gynecologic malignancy, whose incidence rate is on the rise. However, the underlying mechanisms of endometrial cancer are not very clear yet. miRNAs have been considered to be playing important roles in malignant behavior. Here, miR-652 was significantly upregulated in endometrial cancer, which correlated with shorter overall survival and earlier recurrence. Moreover, overexpression of miR-652 in endometrial cancer cells promoted proliferation, migration, and invasion and facilitated tumor growth and metastasis. In contrast, downregulation of miR-652 in endometrial cancer cells inhibited these processes both and. Mechanistically, miR-652 promotes proliferation and metastasis through directly targeting . Both mRNA and protein level of RORA were negatively related with miR-652 and overexpression of RORA can rescue the promotion effect of miR-652. Further experiments indicated miR-652 overexpression can activate the Wnt/β-catenin pathway and RORA can downregulate β-catenin and function as a tumor suppressor in endometrial cancer. Collectively, these findings demonstrate that miR-652 functions as an oncomir in endometrial cancer. IMPLICATIONS: This study suggests that the miR-652 is a critical regulator of proliferation and metastasis in endometrial cancer and may serve as a therapeutic target.

The RNA‐binding protein SORBS2 suppresses hepatocellular carcinoma tumourigenesis and metastasis by stabilizing RORA mRNA

“…Moretti et al confirmed that RORA suppressed prostate cancer cell proliferation by counteracting the effect of fatty acids . Sun et al demonstrated the tumour‐suppressor role of RORA in endometrial cancer to rescue the cancer‐promoting effect of miR‐652 . RORA was also involved in the progression of HCC, and Fu et al demonstrated that decreased RORA expression in HCC tissues indicated worse OS of HCC patients .…”

MicroRNA‐652‐3p promotes the proliferation and invasion of the trophoblast HTR‐8/SVneo cell line by targeting homeobox A9 to modulate the expression of ephrin receptor B4

“…miR‐652‐3p has been reported as a novel disease‐related miRNA dysregulated in various pathological processes . Reportedly, miR‐652‐3p promotes the proliferation, migration, and invasion of various cancer cells . Interestingly, a recent study reports that miR‐652‐3p is essential for follicle development .…”

“…23,[27][28][29] Reportedly, miR-652-3p promotes the proliferation, migration, and invasion of various cancer cells. 22,30 Interestingly, a recent study reports that miR-652-3p is essential for follicle development. 31 However, whether miR-652-3p plays a role in PE development remains unknown.…”

“…miR‐652‐3p has been identified as a novel disease‐associated miRNA that is dysregulated in various pathological processes . However, whether miR‐652‐3p is involved in the pathogenesis of PE remains unknown.…”

“…miR-652-3p has been identified as a novel disease-associated miRNA that is dysregulated in various pathological processes. [22][23][24] However, whether miR-652-3p is involved in the pathogenesis of PE remains unknown. In the present study, our results demonstrate that miR-652-3p is involved in regulating the viability, proliferation, and invasion of trophoblast cells in vitro.…”