miR‐6718‐5p and miR‐4329 can be used as potential biomarkers for acute myocardial infarction

Abstract

Background: The prevention and prognosis of the onset or recurrence of acute myocardial infarction (AMI) is a difficult problem in contemporary research.Methods: In this study, peripheral blood samples were collected from seven patients with AMI and nine healthy adults, and exosome microRNAs (miRNAs) were extracted. The miRNA differential expression profiles of serum exosomes in patients with AMI were obtained by using the next-generation sequencing technology combined with bioinformatics analysis. Reverse-transcriptase polymerase chain reaction (RT-PCR) was used to verify the primary screening of differential exosome miRNAs to reveal the possible mechanism of their action on AMI.Results: Compared with healthy individuals, 544 miRNAs were upregulated and 518 miRNAs were downregulated in AMI patients preoperatively. Among these miRNAs, we selected miR-6718 and miR-4329 for qPCR verification. The expression of miR6718 and miR-4329 in patients with myocardial infarction was significantly lower than that in normal controls.

Unraveling the Signaling Dynamics of Small Extracellular Vesicles in Cardiac Diseases

“…For example, miR-203, miR-4516, or miR-183, which regulate the activity of several protein kinases in CMs, are upregulated, confirming their identification as MI biomarkers [67,70]. Another transcriptomic study identifies the deregulation of miRNA levels in exosomes on a large scale, identifying around 500 upregulated and downregulated miRNAs in MI exosomes, highlighting miR-6718 and miR-4329 [68]. Guo et al (2021) reveal that, in addition to differential expression in exosomal miRNAs identifying up to 18 miRNAs as biomarkers, exosomes differ in size, being smaller in MI patients [69] (Table 1).…”

“…Currently, the main diagnostic biomarker for MI is cardiac troponin (T/I) [64], even though other more sensitive biomarkers have emerged, for example, non-coding RNAs, which allow for an earlier diagnosis [65,66]. Within non-coding RNAs, an increasing number of transcriptomic studies identified miRNAs in exosomes after MI [67][68][69]. Some analyses of circulating exosomes in the serum of MI patients have revealed a large number of miRNAs that are deregulated.…”

miRNA Dysregulation in Cardiovascular Diseases: Current Opinion and Future Perspectives

“…de los Reyes-García et al [ 76 ] reported that miR-146 could be considered a promotor of thromboinflammation and recurrence in young patients with AMI. Chen et al [ 47 ] reported that the expression levels of miR-4329 and miR-6718 were significantly lower in patients with myocardial infarction compared with controls. Based on these data, the authors concluded that these miRNAs could be used as potential biomarkers for AMI.…”

Biological Features of Extracellular Vesicles and Challenges

“…Based on the syber-green PCR array, the three most significantly upregulated miRs including miR-15b, miR-636, and miR-34a were identified in urine exosomes of patients with diabetic kidney disease (Eissa et al, 2016). By using the nextgeneration sequencing technology combined with bioinformatics analysis, 544 upregulated and 518 downregulated exosomal miRs in acute myocardial infarction patients were identified, and miR-6718-5p and miR-4329 can be used as potential diagnostic biomarkers (Chen S. et al, 2021). Using high-throughput sequencing analysis, it was identified a total of 63 miRNAs such as miR-130a-5p were differentially expressed in exosomes from bone marrow mesenchymal stem/stromal cells (MSCs) between diabetic ulcer patients and healthy adults .…”