MiR‐718 represses VEGF and inhibits ovarian cancer cell progression

Leng, Ruobing; Zha, Lang; Tang, Liangdan

doi:10.1016/j.febslet.2014.04.040

Cited by 32 publications

(22 citation statements)

References 27 publications

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“…VEGF expression is an independent prognostic factor in EOC patients (20,21). Aberrantly highly expressed VEGF is involved in EOC onset and development, and it regulates diverse biological processes, including cell proliferation, migration, invasion, apoptosis and angiogenesis (21)(22)(23)(24). Hence, VEGF knockdown using miR-655-based targeted therapy may be an effective therapeutic method for patients with EOC.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑655 inhibits cell proliferation and invasion in epithelial ovarian cancer by directly targeting vascular endothelial growth factor

et al. 2018

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In recent years, microRNAs (miRNAs/miRs) have been shown to be deregulated in epithelial ovarian cancer (EOC). Their deregulation has been suggested to be involved in EOC formation and progression through the regulation of the expression of numerous cancer‑related genes. Hence, it is of great importance to further determine the detailed roles and underlying mechanisms of miRNAs involved in EOC and to identify novel targets for diagnosis, prognosis and treatment of patients with EOC. In this study, the expression of miR‑655‑3p (miR‑655) was significantly downregulated in EOC tissues and four EOC cell lines. After miR‑655 was restored, functional assays revealed that cellular proliferation and invasion were considerably reduced in EOC. Additionally, vascular endothelial growth factor (VEGF) A was identified as a direct target gene of miR‑655 in EOC cells. Furthermore, VEGF knockdown could mimic the tumour‑suppressive roles of miR‑655 overexpression in EOC cells. Moreover, the introduction of VEGF abrogated the effects of miR‑655‑induced proliferation and invasion inhibition in EOC cells. Altogether, these findings indicated that miR‑655 may inhibit EOC cell proliferation and invasion by repressing VEGF. Thus, the miR‑655/VEGF pathway could serve as a novel therapeutic target for patients with EOC.

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Section: Discussionmentioning

confidence: 99%

“…To clarify the mechanism underlying the tumour-suppressive roles of miR-655 in EOC, bioinformatics analysis was performed to predict the putative targets of miR-655. VEGF, a well-known oncogene in EOC (20)(21)(22)(23)(24), was a candidate target gene of miR-655 (Fig. 3A).…”

Section: Vegf Is a Direct Target Gene Of Mir-655 In Eoc Cellsmentioning

confidence: 99%

MicroRNA‑655 inhibits cell proliferation and invasion in epithelial ovarian cancer by directly targeting vascular endothelial growth factor

et al. 2018

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“…Prior reports have suggested that miR-718 is downregulated in ovarian cancer cells and HCC cells (Leng et al, 2014;Sugimachi et al, 2015). miR-718 can exhibit inhibitory effects against ovarian cancer through VEGF repression and it also can suppress cell proliferation in HCC cell lines (Huh7 and PLC/PRF/5) by targeting the HOXB8 gene.…”

Section: Discussionmentioning

confidence: 99%

Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells

Wang¹,

Chen

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Hepatocellular carcinoma (HCC) is still one of the most common death-related malignancies worldwide. Because the way onset and progression are hidden most, HCC diagnoses are made at an advanced stage, when they are unsuitable for surgical resection. MicroRNAs are a class of small non-coding RNAs, participating in many aspects of cancers. In this study, we tried to establish the role of microRNA-718 (miR-718) in the malignant phenotype of HCC cells and its possible role in HCC diagnosis. Methods: Here we first used a methylthiazolyldiphenyltetrazolium bromide (MTT) assay, Transwell migration and invasion assays, and colony formation assay to evaluate the impact of miR-718 on the malignant phenotypes of HCC cells. Then, we used bioinformatic methods to predict the target gene of miR-718 and used green fluorescence protein (GFP) reporter assay, Western blot, and quantitative real-time polymerase chain reaction (qRT-PCR) to validate the regulation relationship. Finally, we determined the role of the target gene in the HCC phenotype. Results: We found that the expression of miR-718 was significantly reduced in various HCC cell lines and HCC tissues. Re-expression of miR-718 significantly reduced the cellular viability and colony formation ability as well as inhibited the migration and invasion abilities of HCC cell lines. Early growth response protein 3 (EGR3) is a direct target of miR-718 and is negatively regulated by miR-718. EGR3 could increase the viability and proliferation of HCC cells, and promot the migration and invasion of HCC cells. Conclusions: miR-718 acts as a tumor suppressive microRNA in HCC via regulating the expression of EGR3, which may provide a new diagnostic marker and treatment target for HCC.

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“…There is increasing evidence that miRNAs are important in vascular development as well as tumor angiogenesis. In response to hypoxic conditions, tumor cells increase the expression of miRNAs that promote angiogenesis and decrease the expression of miRNAs that repress angiogenesis [35]. For instance, MiRNA-27a and miRNA-126 induce angiogenesis by increasing VEGF and VEGFR expression.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…For instance, MiRNA-27a and miRNA-126 induce angiogenesis by increasing VEGF and VEGFR expression. Conversely, miRNA-15b, miRNA-16 and miRNA-20a/b inhibit angiogenesis by modulating levels of VEGF and VEGFR and are down-regulated in tumor cells [35]. MiRNA-718 represses VEGF expression and its restoration inhibits ovarian cancer cell proliferation in vitro and in vivo [36].…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

State of the science: Emerging therapeutic strategies for targeting angiogenesis in ovarian cancer

Graybill¹,

Sood²,

Monk³

et al. 2015

Gynecologic Oncology

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MiR‐718 represses VEGF and inhibits ovarian cancer cell progression

Cited by 32 publications

References 27 publications

MicroRNA‑655 inhibits cell proliferation and invasion in epithelial ovarian cancer by directly targeting vascular endothelial growth factor

MicroRNA‑655 inhibits cell proliferation and invasion in epithelial ovarian cancer by directly targeting vascular endothelial growth factor

Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells

State of the science: Emerging therapeutic strategies for targeting angiogenesis in ovarian cancer

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