MiR-743a-5p regulates differentiation of myoblast by targeting Mob1b in skeletal muscle development and regeneration

Zhang, Yongsheng; Yao, Yilong; Wang, ZiShuai; Lu, Dongfang; Zhang, Yuanyuan; Adetula, Adeyinka Abiola; Liu, SiYuan; Zhu, Min; Yang, Yalan; Fan, Xinhao; Tang, Yijie; Chen, Yun; Liu, Yuwen; Yi, Guoqiang; Tang, Zhonglin

doi:10.1016/j.gendis.2020.11.018

Cited by 11 publications

(8 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MicroRNAs are highly conserved in mammals, and they can negatively regulate gene expression and induce muscle degradation at the post-transcriptional level through the principle of pairing with a complementary base of the 3 ′ non-coding region (UTR) of target mRNA [29]. Previous studies have reported that miR-208b can mediate skeletal muscle development and homeostasis by specifically targeting TCF12 and FNIP1 [30].…”

Section: Discussionmentioning

confidence: 99%

miR-423-5p Regulates Skeletal Muscle Growth and Development by Negatively Inhibiting Target Gene SRF

Pang,

Liang,

Huang

et al. 2024

Genes

View full text Add to dashboard Cite

The process of muscle growth directly affects the yield and quality of pork food products. Muscle fibers are created during the embryonic stage, grow following birth, and regenerate during adulthood; these are all considered to be phases of muscle development. A multilevel network of transcriptional, post-transcriptional, and pathway levels controls this process. An integrated toolbox of genetics and genomics as well as the use of genomics techniques has been used in the past to attempt to understand the molecular processes behind skeletal muscle growth and development in pigs under divergent selection processes. A class of endogenous noncoding RNAs have a major regulatory function in myogenesis. But the precise function of miRNA-423-5p in muscle development and the related molecular pathways remain largely unknown. Using target prediction software, initially, the potential target genes of miR-423-5p in the Guangxi Bama miniature pig line were identified using various selection criteria for skeletal muscle growth and development. The serum response factor (SRF) was found to be one of the potential target genes, and the two are negatively correlated, suggesting that there may be targeted interactions. In addition to being strongly expressed in swine skeletal muscle, miR-423-5p was also up-regulated during C2C12 cell development. Furthermore, real-time PCR analysis showed that the overexpression of miR-423-5p significantly reduced the expression of myogenin and the myogenic differentiation antigen (p < 0.05). Moreover, the results of the enzyme-linked immunosorbent assay (ELISA) demonstrated that the overexpression of miR-423-5p led to a significant reduction in SRF expression (p < 0.05). Furthermore, miR-423-5p down-regulated the luciferase activities of report vectors carrying the 3′ UTR of porcine SRF, confirming that SRF is a target gene of miR-423-5p. Taken together, miR-423-5p’s involvement in skeletal muscle differentiation may be through the regulation of SRF.

show abstract

Section: Discussionmentioning

confidence: 99%

miR-423-5p Regulates Skeletal Muscle Growth and Development by Negatively Inhibiting Target Gene SRF

Pang,

Liang,

Huang

et al. 2024

Genes

View full text Add to dashboard Cite

show abstract

“…In a cell culture model of mouse myoblast differentiation, a downregulation of miR-200c-5p during differentiation has been observed [ 88 ]. The murine Tmigd1 mRNA contains a miR-200c-5p seed sequence in the 3′-UTR, and, importantly, a luciferase reporter system showed a functional interaction of miR-200c-5p with the 3′-UTR of the murine Tmigd1 mRNA in HEK293T cells [ 88 ], making miR-200c-5p a strong candidate for the regulation of TMIGD1 mRNA stability [ 88 ]. Two other candidates are miR-223-3p and miR-680.…”

Section: Regulation Of Tmigd1 Gene Expression: Promoter Methylation T...mentioning

confidence: 99%

TMIGD1: Emerging functions of a tumor supressor and adhesion receptor

et al. 2023

View full text Add to dashboard Cite

The development of multicellular organisms depends on cell adhesion molecules (CAMs) that connect cells to build tissues. The immunoglobulin superfamily (IgSF) constitutes one of the largest families of CAMs. Members of this family regulate such diverse processes like synapse formation, spermatogenesis, leukocyte-endothelial interactions, or epithelial cell-cell adhesion. Through their extracellular domains, they undergo homophilic and heterophilic interactions in cis and trans. Their cytoplasmic domains frequently bind scaffolding proteins to assemble signaling complexes. Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) is a IgSF member with two Ig-like domains and a short cytoplasmic tail that contains a PDZ domain-binding motif. Recent observations indicate that TMIGD1 has pleiotropic functions in epithelial cells and has a critical role in suppressing malignant cell behavior. Here, we review the molecular characteristics of TMIGD1, its interaction with cytoplasmic scaffolding proteins, the regulation of its expression, and its downregulation in colorectal and renal cancers.

show abstract

“…In addition, a study has demonstrated that miR-27a-3p and miR-142-3p play a regulatory role in skeletal muscle lipid utilization [ 37 ]. Our group previously found that miR-143a, miR-133a, miR-743a-5p, and miR-200c can regulate myogenesis and skeletal muscle regeneration by regulating skeletal muscle cell proliferation, differentiation, and migration [ 38 , 39 , 40 , 41 ]. However, the roles of miRNA in myogenesis, skeletal muscle regeneration, and muscle type fiber transformation are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle

Fan,

Yao,

Liu

et al. 2024

Genes

Self Cite

View full text Add to dashboard Cite

Skeletal muscle plays critical roles in providing a protein source and contributing to meat production. It is well known that microRNAs (miRNAs) exert important effects on various biological processes in muscle, including cell fate determination, muscle fiber morphology, and structure development. However, the role of miRNA in skeletal muscle development remains incompletely understood. In this study, we observed a critical miRNA, miR-24-3p, which exhibited higher expression levels in Tongcheng (obese-type) pigs compared to Landrace (lean-type) pigs. Furthermore, we found that miR-24-3p was highly expressed in the dorsal muscle of pigs and the quadriceps muscle of mice. Functionally, miR-24-3p was found to inhibit proliferation and promote differentiation in muscle cells. Additionally, miR-24-3p was shown to facilitate the conversion of slow muscle fibers to fast muscle fibers and influence the expression of GLUT4, a glucose transporter. Moreover, in a mouse model of skeletal muscle injury, we demonstrated that overexpression of miR-24-3p promoted rapid myogenesis and contributed to skeletal muscle regeneration. Furthermore, miR-24-3p was found to regulate the expression of target genes, including Nek4, Pim1, Nlk, Pskh1, and Mapk14. Collectively, our findings provide evidence that miR-24-3p plays a regulatory role in myogenesis and fiber type conversion. These findings contribute to our understanding of human muscle health and have implications for improving meat production traits in livestock.

show abstract

MiR-743a-5p regulates differentiation of myoblast by targeting Mob1b in skeletal muscle development and regeneration

Cited by 11 publications

References 40 publications

miR-423-5p Regulates Skeletal Muscle Growth and Development by Negatively Inhibiting Target Gene SRF

miR-423-5p Regulates Skeletal Muscle Growth and Development by Negatively Inhibiting Target Gene SRF

TMIGD1: Emerging functions of a tumor supressor and adhesion receptor

Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle

Contact Info

Product

Resources

About