miR-762 promotes porcine immature Sertoli cell growth via the ring finger protein 4 (RNF4) gene

Ma, Changping; Song, Huibin; Yu, Lei; Guan, Kaifeng; Hu, Pandi; Li, Yang; Xia, Xuanyan; Li, Jialian; Jiang, Siwen; Li, Fenge

doi:10.1038/srep32783

Cited by 44 publications

(53 citation statements)

References 37 publications

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“…Considering the crucial role of immature SC in spermatogenesis, miRNAs act as important regulators in SC proliferation, maturation, and barrier regulation via the modulation of transcription factors involved in the cell cycle (Jiao et al, ; Ma et al, ; Yao et al, ). miR‐133b promoted SC proliferation by directly targeting the transcription factor GLI family zinc finger 3 and increased cyclin B1 and cyclin D11 expressions (Yao et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…miR‐133b promoted SC proliferation by directly targeting the transcription factor GLI family zinc finger 3 and increased cyclin B1 and cyclin D11 expressions (Yao et al, ). Ma et al () reported that miR‐762 mimic‐treated immature SCs promoted the proliferation through directly binding the 3ʹ‐UTR of ring finger protein 4, and Jiao et al () used a miR‐1285 mimic to promote SC proliferation through distinct pathways associated with the cell cycle. In addition, miR‐471 was found to be involved in SC metabolism and barrier function through downregulating desmocollin‐1 and forkhead box D1 (Panneerdoss et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Specific expression of miRNAs is found in different periods of mouse testes through gene chip and miRscan, and it has been confirmed that follicle stimulating hormone and androgen can regulate male reproductive activity through the expression of miRNAs in SCs (Mishima et al, ; Nicholls et al, ; Wu, Bao, Wang, Hu, & Xu, ; Yan et al, ). Recent studies have demonstrated that miRNAs repress the expression of SC target genes, thereby regulating SC proliferation, maturation, apoptosis, blood‐testis barrier, and stages of the seminiferous epithelium cycle (Jiao et al, ; Ma et al, ; Yao et al, ). Specific knockdown of Dicer, a central component of RNA interference machinery, downregulates the expression of several key genes in SCs and results in male infertility because of spermatozoa absence and progressive testicular degeneration (Papaioannou et al, ; Papaioannou et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Identification of microRNAs for regulating adenosine monophosphate‐activated protein kinase expression in immature boar Sertoli cells in vitro

Zhang

Yang

Wang

et al. 2019

Molecular Reproduction Devel

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Adenosine monophosphate‐activated protein kinase (AMPK) plays a key role in cellular energy homeostasis and cell proliferation. MicroRNAs (miRNAs) function as posttranscriptional regulators of gene expression in biological processes. It is unclear to whether miRNAs are involved in AMPK‐regulated Sertoli cell (SC) proliferation. To further understand the regulation of miRNAs in the immature boar SC proliferation, 5‐aminoimidazole‐4‐carboxamide‐1‐β‐D‐ribofuranoside (AICAR) was added to activate AMPK. By an Illumina small RNA deep sequencing, we obtained sequences and relative expression levels of 272 known mature miRNAs, among which 9 miRNAs were significantly upregulated whereas 16 miRNAs were downregulated following the AICAR treatment. The results identified 38 conserved miRNAs, with 8 significantly downregulated miRNAs whereas no upregulated miRNAs. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses suggested that miR‐1285 was involved in many activities and pathways associated with cell proliferation via targeting on AMPKα2. We validated that AICAR significantly downregulated miR‐1285 level in SCs. Transfection of miR‐1285 mimic increased the SC viability and cell cycle progression but reduced AMPKα2 mRNA and protein levels, indicating that miR‐1285 is involved in the immature boar SC proliferation via downregulating AMPKα2 expression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of microRNAs for regulating adenosine monophosphate‐activated protein kinase expression in immature boar Sertoli cells in vitro

Zhang

Yang

Wang

et al. 2019

Molecular Reproduction Devel

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“…Recent evidence suggests that spermatogenesis is strictly regulated post‐transcriptionally, with small RNAs as the key regulators at this level (Mccubbin et al., ; Tang, Huang, Liu, & Zhang, ). For instance, miR‐20a might be a key inhibitor in the genistein‐induced slight abnormal spermatogenesis both in human and mice (Gu et al., ), and miR‐31 targets stimulated by retinoic acid gene 8 (Stra8) and inhibits spermatogenesis in spermatogonial stem cells (Wang et al., ), and miR‐17‐92 and miR‐762 in mice and pigs (Ma et al., ; Xie et al., ).…”

Section: Discussionmentioning

confidence: 99%

Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next‐generation sequencing

Zhong

Chai

et al. 2018

Reprod Domestic Animals

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microRNA (miRNA) and piwi-interacting RNA (piRNA) are two classes small non-coding regulatory RNAs that play crucial roles in multiple biological processes such as spermatogenesis. However, there are no published studies on conjoint analysis of miRNA and piRNA profiles among cattle, yak and their interspecies (the dzo) using sequencing technology. Next-generation sequencing technology was used to profile miRNAs and piRNAs among those three ruminants to elucidate their functions. A total of 119, 14 and six differentially expressed miRNAs were obtained in cattle vs. dzo, cattle vs. yak and yak vs. dzo comparison groups, while there were 873, 1,065 and 1,158 differentially expressed piRNAs in those three comparison groups. The expression of three miRNAs was validated in the three ruminants, and the results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, the putative targets of differentially expressed miRNAs were predicted by their own genome, it is worth to note that both the cattle and yak genome were used for dzo, then the targets were subjected to GO enrichment and KEGG pathway analysis, revealing the likely roles for these differentially expressed miRNAs in spermatogenesis. In conclusion, this study provided a useful resource for further elucidation of the miRNAs and piRNAs regulatory roles in spermatogenesis. It may also facilitate the development of therapeutic strategies for dzo reproduction research.

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“…Swine testicular (ST) cells were purchased from BOSTER (ATCC ® CRL‐2528 ™ , Wuhan, China). ST cells were identified as porcine immature Sertoli cells and used in researches related to Sertoli cell function and maturation (Ma, Song, Guan, et al., ; Ma, Song, Yu, et al., ). Cells were cultured in high‐glucose Dulbecco’s modified Eagle’s medium (Hyclone, USA) with 10% foetal bovine serum (Gibco, Grand Island, NY, USA) at 37°C with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

miR‐26a suppresses autophagy in swine Sertoli cells by targeting ULK2

Ran

Cao

et al. 2018

Reprod Domestic Animals

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A large number of microRNAs (miRNAs) have been detected from porcine testicular tissues thanks to the development of high-throughput sequencing technology. However, the regulatory roles of most identified miRNAs in swine testicular development or spermatogenesis are poorly understood. In our previous study, ULK2 (uncoordinated-51-like kinase 2) was predicted as a target gene of miR-26a. In this study, we aimed to investigate the role of miR-26a in swine Sertoli cell autophagy. The relative expression of miR-26a and ULK2 levels has a significant negative correlation (R = .5964, p ≤ .01) in nine developmental stages of swine testicular tissue. Dual-luciferase reporter assay results show that miR-26a directly targets the 3'UTR of the ULK2 gene (position 618-624). In addition, both the mRNA and protein expression of ULK2 were downregulated by miR-26a in swine Sertoli cells. These results indicate that miR-26a targets the ULK2 gene and downregulates its expression in swine Sertoli cells. Based on the expression of marker genes (LC3, p62 and Beclin-1), overexpression of miR-26a or knock-down of ULK2 inhibits swine Sertoli cell autophagy. Taken together, these findings demonstrate that miR-26a suppresses autophagy in swine Sertoli cells by targeting ULK2.

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miR-762 promotes porcine immature Sertoli cell growth via the ring finger protein 4 (RNF4) gene

Cited by 44 publications

References 37 publications

Identification of microRNAs for regulating adenosine monophosphate‐activated protein kinase expression in immature boar Sertoli cells in vitro

Identification of microRNAs for regulating adenosine monophosphate‐activated protein kinase expression in immature boar Sertoli cells in vitro

Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next‐generation sequencing

miR‐26a suppresses autophagy in swine Sertoli cells by targeting ULK2

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