miR‑885‑5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling

Abstract

The aim of the present study was to investigate the role of microRNA (miR)-885-5p in colorectal cancer cell proliferation and migration, and to determine the possible underlying molecular mechanisms. The expression of miR-885-5p in colorectal cancer tissue and cells was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression levels of three suppressor of cytokine signaling (SOCS) factors were detected by RT-qPCR and western blotting. The effects of miR-885-5p on tumor cell proliferation and migration were studied using MTT and Transwell assays, respectively. Additionally, the expression levels of epithelial-mesenchymal transition (EMT)-related proteins (N-cadherin, E-cadherin, vimentin and Snail) were detected by RT-qPCR and western blot analysis. Furthermore, the target of miR-885-5p was predicted and confirmed using a luciferase reporter assay. miR-885-5p was demonstrated to be upregulated and SOCS was downregulated in colorectal cancer tissue, and cells. miR-885-5p suppression significantly inhibited tumor cell proliferation and migration, promoted E-cadherin expression, and inhibited the expression levels of N-cadherin, vimentin and Snail. Further studies showed that SOCS5, SOCS6 and SOCS7 were direct targets of miR-885-5p. The results suggest that miR-885-5p suppression inhibited cell proliferation and migration, and the EMT process by targeting SOCS5, SOCS6 and SOCS7 genes in colorectal cancer. miR-885-5p and SOCS may be used for the diagnosis and treatment of colorectal cancer.

Long non‐coding RNA HCG11 sponging miR‐522‐3p inhibits the tumorigenesis of non‐small cell lung cancer by upregulating SOCS5

“…Su et al also reported that miR-885-5p upregulation promoted colorectal cancer cell proliferation and migration by targeting suppressor of SOCS5. 31 Additionally, lncRNA HAND2-AS1 has been reported to inhibit liver cancer cell proliferation and migration by upregulating SOCS5. 32 Here, downregulation of HCG11 weakened the inhibitory effect of SOCS5 in NSCLC, indicating that lncRNA HCG11 restrains NSCLC progression by upregulating SOCS5.…”

miRNA profiles of canine cutaneous mast cell tumours with early nodal metastasis and evaluation as potential biomarkers

“…MiR-338 is also involved in hypoxia-induced epithelial-to-mesenchymal transition by targeting HIF-1α 45 . MiR-885 is a tumour suppressor miRNA that interferes with cell proliferation and migration by targeting SOCS in colorectal cancer 46 and the Wnt/β-catenin pathway in hepatocellular carcinoma 47 .…”

Duck Tembusu Virus Utilizes miR-221-3p Expression to Facilitate Viral Replication via Targeting of Suppressor of Cytokine Signaling 5

“…Currently, a growing body of evidence has demonstrated that miRNAs level negatively correlated with SOCS protein expression (Peng et al, 2011;Jun et al, 2012;Yao et al, 2012;Wang X. et al, 2019). For instance, the expression of SOCS5 was inhibited by several miRNAs, such as miR-432, miR-301a, miR-130b, miR-124, miR-33-5p, miR-885-5p, and miR-132 (Shan et al, 2014;Sharma et al, 2016;Fu et al, 2017;Hazra et al, 2017;Diao et al, 2018;Su et al, 2018;Tsai et al, 2018). miR-432 inhibited JEV replication via regulating JAK-STAT signaling by targeting SOCS5, but another report indicated that miR-301a enhanced JEV replication through regulating EGFR signaling via targeting SOCS5 (Sharma et al, 2016;Hazra et al, 2017).…”