MiR-9-3p augments apoptosis induced by H2O2 through down regulation of Herpud1 in glioma

Yang, Ling; Mu, Yongping; Cui, Hongwei; Liang, Yabing; Su, Xiulan

doi:10.1371/journal.pone.0174839

Cited by 26 publications

(23 citation statements)

References 38 publications

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“…Glioma, a kind of malignancy arising from glial cells in the brain, is the most common malignant tumours occurring in the central nervous system and accounting for 78% of intracranial primary tumours 1, 2. Glioma usually exhibits a series of malignant features, including extensive, rapid and aggressive progression, resistance to surgery, radiotherapy and chemotherapy, all of which lead to the poor prognosis for most patients 3.…”

Section: Introductionmentioning

confidence: 99%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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This study was designed to explore the relationship between miR‐1275 and SERPINE1 and its effects on glioma cell proliferation, migration, invasion and apoptosis. Differentially expressed miRNAs and mRNAs in glioma tissues were screened out by bioinformatic analysis. Dual‐luciferase reporter gene assay was used to validate the targeted relationship between miR‐1275 and SERPINE1. qRT‐PCR was used to detect the expression of miR‐1275 and SERPINE1 in glioma tissues. The expressions of SERPINE1 and p53 pathway‐related proteins in glioma cells were detected by western blot. Glioma cell proliferation, apoptosis, migration and invasion were respectively detected by CCK‐8 assay, flow cytometry, wound healing assay and transwell assay. Tumour xenograft model was developed to study the influence of miR‐1275 and SERPINE1 on glioma growth in vivo. The results of microarray analysis, qRT‐PCR and western blot showed that miR‐1275 was low‐expressed while SERPINE1 was high‐expressed in glioma. Dual‐luciferase assay showed that miR‐1275 could bind to SERPINE1. Overexpression of miR‐1275 could promote the p53 pathway‐related proteins’ expression. Highly expressed miR‐1275 could repress the migration, proliferation and invasion of glioma cells while highly expressed SERPINE1 had inverse effects. Tumour xenograft showed that up‐regulated miR‐1275 or down‐regulated SERPINE1 could repress glioma growth in vivo. Up‐regulation of miR‐1275 activated p53 signalling pathway via regulating SERPINE1 and therefore suppressed glioma cell proliferation, invasion and migration, whereas promoted cell apoptosis.

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Section: Introductionmentioning

confidence: 99%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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“…miR-9-3p has been demonstrated to regulate biological functions in multiple types of cancer. For example, Yang et al (33) reported that H 2 O 2 -induced apoptosis of glioma cells was facilitated by miR-9-3p through downregulation of Herpud1 expression. In another study, miR-9-3p inhibited cell proliferation by targeting the PDZ-binding motif TAZ in hepatocellular carcinoma (34).…”

Section: Discussionmentioning

confidence: 99%

Regulatory interactions between long noncoding RNA LINC00968 and miR‑9‑3p in non‑small cell lung cancer: A bioinformatic analysis based on miRNA microarray, GEO and TCGA

Chen

Shang

et al. 2018

Oncol Lett

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Long non-coding RNAs (lncRNAs) have been demonstrated to mediate carcinogenesis in various types of cancer. However, the regulatory role of lncRNA LINC00968 in lung adenocarcinoma remains unclear. The microRNA (miRNA) expression in LINC00968-overexpressing human lung adenocarcinoma A549 cells was detected using miRNA microarray analysis. miR-9-3p was selected for further analysis, and its expression was verified in the Gene Expression Omnibus (GEO) database. In addition, the regulatory axis of LINC00968 was validated using The Cancer Genome Atlas (TCGA) database. Results of the GEO database indicated miR-9-3p expression in lung adenocarcinoma was significantly higher compared with normal tissues. Functional enrichment analyses of the target genes of miR-9-3p indicated protein binding and the AMP-activated protein kinase pathway were the most enriched Gene Ontology and KEGG terms, respectively. Combining target genes with the correlated genes of LINC00968 and miR-9-3p, 120 objective genes were obtained, which were used to construct a protein-protein interaction (PPI) network. Cyclin A2 (CCNA2) was identified to have a vital role in the PPI network. Significant correlations were detected between LINC00968, miR-9-3p and CCNA2 in lung adenocarcinoma. The LINC00968/miR-9-3p/CCNA2 regulatory axis provides a new foundation for further evaluating the regulatory mechanisms of LINC00968 in lung adenocarcinoma.

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“…Low expression of miR-9-3p leads to a high level of Herpud 1, which prevents glioma cell apoptosis [ 61 ]. The function of Herpud1 is not totally figured out, but there is more and more evidence that it plays an important role in ER-membrane-associated protein degradation (ERAD) by transferring ubiquitin protein from endoplasmic reticulum (ER) to proteasome degradation [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

The biological functions of target genes in pan-cancers and cell lines were predicted by miR-375 microarray data from GEO database and bioinformatics

et al. 2018

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BackgroundMicroRNA is endogenous non-coding small RNA that negative regulate and control gene expression, and increasing evidence links microRNA to oncogenesis and the pathogenesis of cancer. The goal of this study was to explore the potential molecular mechanism of miR-375 in various cancers.MethodsMiR-375 overexpression in different tumor cell lines was probed with microarray data from Gene Expression Omnibus (GEO). The common target genes of miR-375 were obtained by Robust Rank Aggregation (RRA), and identified by miRWalk2.0 software for target gene prediction. Additionally, we directed in silico analysis including Protein-Protein Interactions (PPI) analysis, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways annotations to provide a summary of the function of miR-375 in various carcinomas. Eventually, data was obtained from The Cancer Genome Atlas (TCGA) were utilized for a validation in 7 cancers.ResultsThe nine miR-375 related chips were acquired by the GEO data. The 5 down regulated genes came from 9 available microarray datasets, which overlapped with the potential target genes predicted by miRWalk2.0 software. The target genes were intensely enriched in amino acid biosynthetic and metabolic process from biological process (GO) and Cysteine and methionine metabolism (KEGG analysis). In view of these approaches, VASN, MAT2B, HERPUD1, TPAPPC6B and TAT are probably the most important miR-375 targets. In addition, miR-375 was negatively correlated with MAT2B, which was verified in 5 tumors of TCGA.ConclusionIn summary, this study based on common target genes provides an innovative perspective for exploring the molecular mechanism of miR-375 in human tumors.

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MiR-9-3p augments apoptosis induced by H2O2 through down regulation of Herpud1 in glioma

Cited by 26 publications

References 38 publications

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Regulatory interactions between long noncoding RNA LINC00968 and miR‑9‑3p in non‑small cell lung cancer: A bioinformatic analysis based on miRNA microarray, GEO and TCGA

The biological functions of target genes in pan-cancers and cell lines were predicted by miR-375 microarray data from GEO database and bioinformatics

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