miR-99a-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting MTMR3 in Chicken

Cao, Xinao; Tang, Shuyue; Du, Fei; Li, Hao; Shen, Xiaoxu; Li, Diyan; Wang, Yan; Zhang, Zhichao; Xia, Lu; Zhu, Qing; Yin, Huadong

doi:10.3390/genes11040369

Cited by 25 publications

(23 citation statements)

References 32 publications

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“…miR-186 is also an important myogenic miRNAs, which inhibits the differentiation of C2C12 cells and primary muscle cells by targeting myogenin [52]. miR-99 targets myotubularinrelated protein 3 (MTMR3) and regulates chicken skeletal muscle satellite cells' proliferation and differentiation [53]. Involvement of these miRNAs in muscle growth explains the significant correlation between the expression of these miRNAs in LDM and fetal weight in pigs at 63 dpc.…”

Section: Discussionmentioning

confidence: 99%

In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes

Ali

Muráni

Hadlich

et al. 2021

Genes

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Impaired skeletal muscle growth in utero can result in reduced birth weight and poor carcass quality in pigs. Recently, we showed the role of microRNAs (miRNAs) and their target genes in prenatal skeletal muscle development and pathogenesis of intrauterine growth restriction (IUGR). In this study, we performed an integrative miRNA-mRNA transcriptomic analysis in longissimus dorsi muscle (LDM) of pig fetuses at 63 days post conception (dpc) to identify miRNAs and genes correlated to fetal weight. We found 13 miRNAs in LDM significantly correlated to fetal weight, including miR-140, miR-186, miR-101, miR-15, miR-24, miR-29, miR-449, miR-27, miR-142, miR-99, miR-181, miR-199, and miR-210. The expression of these miRNAs decreased with an increase in fetal weight. We also identified 1315 genes significantly correlated to fetal weight at 63 dpc, of which 135 genes were negatively correlated as well as identified as potential targets of the above-listed 13 miRNAs. These miRNAs and their target genes enriched pathways and biological processes important for fetal growth, development, and metabolism. These results indicate that the transcriptomic profile of skeletal muscle can be used to predict fetal weight, and miRNAs correlated to fetal weight can serve as potential biomarkers of prenatal fetal health and growth.

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Section: Discussionmentioning

confidence: 99%

In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes

Ali

Muráni

Hadlich

et al. 2021

Genes

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“…From previous studies, it is not difficult to see that most of the researches on miRNA have focused on their ability to regulate cellular processes by targeting different genes, while many current studies also show that a single miRNA can have multiple target genes (Kim et al, 2008 ; Cao et al, 2020 ), as is the case with gga-miR-148a-5p. In order to explore which genes was targeted by gga-miR-148a-5p to mediate the proliferation and cell cycle of ALV-J-infected CEF cells, bioinformatics analysis was performed using two different software tools, TargetScan and miRDB, and 92 most likely target gene were identified.…”

Section: Discussionmentioning

confidence: 99%

gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells

Yan

et al. 2020

Front. Cell Dev. Biol.

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Avian leukosis virus subgroup J disease (ALV-J) is a contagious and immunosuppressive avian disease caused by ALV-J virus. Although miRNA participate in various biological processes of tumors, little is known about the potential role of miRNA in ALV-J. Our previous miRNA and RNA sequencing data showed that the expression of gga-miR-148a-5p was significantly different in ALV-J-infected chicken spleens compared with non-infected chickens. The aim of this study was to investigate the functional roles of gga-miR-148a-5p and identify downstream targets regulated by gga-miR-148a-5p in ALV-J-infected chickens. We found that the expression of gga-miR-148a-5p was significantly downregulated during ALV-J infection of chicken embryo fibroblasts (CEF). Dual luciferase reporter assays demonstrated that PDPK1 is a direct target gene of gga-miR-148a-5p. In vitro, overexpression of gga-miR-148a-5p significantly promoted ALV-J-infected CEF cell proliferation, included cell cycle, whereas inhibition of gga-miR-148a-5p had an opposite effect. Inhibition of PDPK1 promoted the proliferation of ALV-J-infected cells but had no effect on the activity of NF-κB. Together, these results suggested that gga-miR-148a-5p targets PDPK1 to inhibit the proliferation and cell cycle of ALV-J-infected CEF cells. Our study provides a new understanding for the tumor mechanism of ALV-J infection.

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“…MiR-99a-5p is also reported to regulate proliferation and differentiation of satellite cells in chickens. MiR-99a-5p targets the 3' UTR region of myotubularin-related protein 3 (MTMR3) and knockdown of MTMR3 by miR-99a-5p promotes proliferation and inhibits differentiation of chicken satellite cells (99). Recently, miR-146b-3p was shown to regulate myogenesis in the chicken.…”

Section: Expression and Function Of Mirnas In The Skeletal Muscle Of mentioning

confidence: 99%

Tiny Regulators of Massive Tissue: MicroRNAs in Skeletal Muscle Development, Myopathies, and Cancer Cachexia

2020

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Skeletal muscles are the largest tissues in our body and the physiological function of muscle is essential to every aspect of life. The regulation of development, homeostasis, and metabolism is critical for the proper functioning of skeletal muscle. Consequently, understanding the processes involved in the regulation of myogenesis is of great interest. Non-coding RNAs especially microRNAs (miRNAs) are important regulators of gene expression and function. MiRNAs are small (~22 nucleotides long) noncoding RNAs known to negatively regulate target gene expression post-transcriptionally and are abundantly expressed in skeletal muscle. Gain- and loss-of function studies have revealed important roles of this class of small molecules in muscle biology and disease. In this review, we summarize the latest research that explores the role of miRNAs in skeletal muscle development, gene expression, and function as well as in muscle disorders like sarcopenia and Duchenne muscular dystrophy (DMD). Continuing with the theme of the current review series, we also briefly discuss the role of miRNAs in cancer cachexia.

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miR-99a-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting MTMR3 in Chicken

Cited by 25 publications

References 32 publications

In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes

In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes

gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells

Tiny Regulators of Massive Tissue: MicroRNAs in Skeletal Muscle Development, Myopathies, and Cancer Cachexia

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