MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells

Zhong, Yuyi; Li, Liying; Chen, Zitao; Diao, Shuqi; He, Yingting; Zhang, Zhe; Zhang, Hao; Yuan, Xiaolong; Li, Jiaqi

doi:10.3389/fcell.2020.565261

Cited by 12 publications

(7 citation statements)

References 66 publications

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“…Одной из ключевых особенностей ЭМП является потеря целостности эпителия, осуществляемая за счет протеолитического расщепления матричными металлопротеиназами (ММП), экспрессия которых регулируется hsa-miR-21-5p, hsa-miR-143-3p и hsa-miR-26a-5p. Известно, что hsa-miR-21-5p и hsa-miR-26a-5p положительно модулируют транскрипцию ММП-2 и ММП-14 соответственно, тогда как hsa-miR-143-3p ингибирует экспрессию ММП-14 [28]. Ранее сообщалось, что hsa-miR-21 способствовала миграции и инвазии опухолевых клеток за счет усиления экспрессии ММП-2 и ММП-9 при гепатоцеллюлярной карциноме [29].…”

Section: Discussionunclassified

MicroRNA expression profile in isolated circulating tumor cells in colorectal cancer

et al. 2023

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Introduction. Colorectal cancer is a frequently diagnosed disease being in the third place among oncological diseases both in incidence and mortality. Currently, researchers focus on development of more accessible and reliable biomarkers of colorectal cancer to overcome the problems in diagnosis and progression prognosis of this pathology.Aim. To investigate characteristics of microRNA expression in circulating tumor cells (CTC) of patients with colorectal cancer. Materials and methods. The study included blood samples from 299 patients with colon cancer, stages II (T3–4N0M0), III (T1–4N1–2M0) and IV (T1–4N0–2M1). Circulating tumor cells were identified using EpCAM marker detection system. Relative expression of hsa-let-7i-5p, hsa-miR-126-5p, hsa-miR-143-3p, hsa-miR-21-5p, hsa-miR-25-3p, hsa-miR-26a-5p, hsa-miR-92a-3p micro RNA in CTC was measured using polymerase chain reaction.Results. Positive CTC status was observed in 188 (62.9 %) of 299 patients, negative in 111 (37.1 %). In the patient group with pT1–2 tumors, the majority of patients did not have CTC (53.3 %). In other patients with pT1–2 disease, the number of CTC was 1.2 and 4.4 times lower than in patients with pT3 and pT4 disease, respectively. In pT4, 1–3 CTC were found 2.7 and 1.7 times more frequently, 3 CTC 1.4 times more frequently than in pT1–2 and pT3, respectively (p ≤0.05). Presence of metastatic lesions increases the probability of CTC detection by the factor of 2.1: in metastases, >3 CTC were observed 60.1 times more frequently than in M0 (p ≤0.05). Expression of hsa-miR-143-3p and hsa-miR-26a-5p microRNA in CTC of patients with stage III colorectal cancer was respectively 2.5 and 5 times lower than in patients with stage II disease (p <0.05) and expression of hsa-miR-21-5p and hsa-miR-92a-3p microRNA was respectively 3.2 and 3 times higher (p <0.05). In CTC of patients with stage IV colorectal cancer, the relative level of expression of hsa-miR-143-3p and hsa-miR-26a-5p was respectively 4.6 and 5.3 times lower (p <0.05) compared to the level of expression in stage II disease, and hsa-miR-126-5p, hsa-miR-21-5p, hsa-miR-25-3p and hsa-miR-92a-3p expression levels were respectively 2.6, 4.6, 2.6 and 5.0 times higher (p<0.05) (statistically significant results).Conclusion. The level of microRNA expression in CTC can be used for differential diagnosis of regional and distant metastases.

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Section: Discussionunclassified

MicroRNA expression profile in isolated circulating tumor cells in colorectal cancer

et al. 2023

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“…MiR-143-3p is significantly low expressed in gastric cancer and lung cancer tissues, negatively regulates the occurrence and development of tumors, promotes the growth and metastasis of tumor cells, and plays an obvious role in tumor suppressor genes (He et al, 2020;Lu et al, 2020). Meanwhile, miR-143-3p has been reported to induce apoptosis in granulosa and rectal cancer cells (Zhong et al, 2020;Shan et al, 2021). Therefore, we investigated the effects of LINC00511/miR-143-3p on the proliferation, invasion, and apoptosis of bladder cancer cells.…”

Section: Discussionmentioning

confidence: 99%

LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1

Dong

Zhang

Mao

et al. 2021

Front. Cell Dev. Biol.

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Bladder cancer has easy recurrence characteristics, but its occurrence and development mechanism are still unclear. Non-coding RNA is a kind of RNA that exists widely and cannot be translated into proteins, which has played a key role in the regulation of biological functions of tumor cells. However, the regulation mechanism of non-coding RNA on bladder tumors is not fully understood. By microarray analysis and database analysis, we found that LINC00511 was significantly highly expressed in bladder cancer. The expressions of LINC00511, miR-143-3p, and PCMT in bladder cancer tissues and cells were detected by quantitative reverse transcription–polymerase chain reaction. The relationship between the expressions of miR-143-3p and PCMT1 and the clinicopathological parameters of the tumor was analyzed. The proliferation and invasion of bladder cancer cells were detected by MTT assay and Transwell assay. The expression levels of E-cadherin and vimentin in bladder cancer cells were detected by Western blot. Cell apoptosis was detected by flow cytometry. In vivo, TCCSUP or SW780 cells were inoculated into BALB/c nude mice to detect tumor volume and weight. Bioinformatics and dual luciferase reporter gene were used to analyze the relationship between LINC00511 and miR-143-3p and its downstream target gene PCMT1. The results showed that LINC00511 could target miR-143-3p/PCMT1 to regulate the proliferation, migration, and apoptosis of bladder cancer TCCSUP or SW780 cells and promote the occurrence and development of bladder cancer.

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“…The candidates from two softwares were intersected to obtain the final circRNAs dataset based on chromosome location. The sequence splicing of circRNAs and the visualization of ring construction diagram were performed by using CIRI-full and CIRI-vis, respectively [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

CircRNAs in Xiang pig ovaries among diestrus and estrus stages

Niu

Huang

et al. 2022

Porc Health Manag

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Background The fecundity of sows is a trait of major economic in pig industry. The molecular regulation of estrus cycles can affect the fecundity of female animals. Compared with the other pig breeds, Xiang pig exhibits the special estrus behaviors. CircRNAs are thought to involve in regulation of multiple biological processes. However, the potential roles of circRNAs in ovary regulation on Xiang pig estrus are largely unknown. Results 8,937 circRNAs were identified from eight libraries constructed from the ovarian samples of Xiang pig at estrus and diestrus stages by RNA sequencing method. Of which, 1,995 were high confidence circRNAs detected at least two junction reads in each ovary sample and seven circRNAs were validated by RT-PCR method. Furthermore, we identified 290 upregulated and 15 downregulated circRNAs in estrus ovaries. These differentially expressed circRNAs (DECs) derived from 273 host genes. And 207 miRNAs were identified to be targets sponged by 156 DECs with 432 binding sites, containing more than one miRNA binding site in each circRNA. Function enrichment analysis revealed that the host genes and the targets of miRNAs sponged by DECs were enriched in several reproduction-related signaling pathways, such as ovarian steroidogenesis, oocyte maturation, circadian rhythm, estrogen signaling pathway, GnRH signaling pathway, circadian entrainment, and oocyte meiosis. The circRNA-miRNA-mRNA networks revealed that 153 miRNAs interacting with 122 DECs and 86 miRNAs interacting with 84 DECs were involved in ovarian functions and ovarian circadian entrainment and circadian rhythm respectively. The DEC-miRNA-DEG (differentially expressed gene, DEG) networks associated with reproduction-related signaling pathways contained 22 DECs,18 miRNAs and 7 DEGs. 22 DECs were recognized as hub circRNAs during the estrus phase of Xiang pigs. Conclusions The circRNAs that function as miRNA sponges could play a key role in post-transcriptional regulation of gene expression during Xiang pig’s estrus cycle.

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MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells

Cited by 12 publications

References 66 publications

MicroRNA expression profile in isolated circulating tumor cells in colorectal cancer

MicroRNA expression profile in isolated circulating tumor cells in colorectal cancer

LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1

CircRNAs in Xiang pig ovaries among diestrus and estrus stages

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