MiR858-Mediated Regulation of Flavonoid-Specific MYB Transcription Factor Genes Controls Resistance to Pathogen Infection in Arabidopsis

Camargo-Ramírez, Rosany; Val-Torregrosa, Beatriz; Segundo, Blanca San

doi:10.1093/pcp/pcx175

Cited by 80 publications

(44 citation statements)

References 55 publications

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“…RNS1 expression is also associated to wounding induced local and systemic signaling (Lebrasseur et al, 2002), and correlates with the accumulation of specific tRNA fragments (Megel et al, 2019). The role of the other identified ribonuclease, PR4, is less known, its expression is associated with SAR (Mishina and Zeier, 2007), and its induced by ethylene and SA (Potter et al, 1993;Camargo-Ramírez et al, 2018).…”

Section: Proteins That Accumulate In the Apoplast Of Senescent Leavesmentioning

confidence: 99%

Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

et al. 2020

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The apoplast, i.e. the cellular compartment external to the plasma membrane, undergoes important changes during senescence. Apoplastic fluid volume increases quite significantly in senescing leaves, thereby diluting its contents. Its pH elevates by about 0.8 units, similar to the apoplast alkalization in response to abiotic stresses. The levels of 159 proteins decrease, whereas 24 proteins increase in relative abundance in the apoplast of senescing leaves. Around half of the apoplastic proteins of non-senescent leaves contain a N-terminal signal peptide for secretion, while all the identified senescence-associated apoplastic proteins contain the signal peptide. Several of the apoplastic proteins that accumulate during senescence also accumulate in stress responses, suggesting that the apoplast may constitute a compartment where developmental and stress-related programs overlap. Other senescence-related apoplastic proteins are involved in cell wall modifications, proteolysis, carbohydrate, ROS and amino acid metabolism, signaling, lipid transport, etc. The most abundant senescence-associated apoplastic proteins, PR2 and PR5 (e.g. pathogenesis related proteins PR2 and PR5) are related to leaf aging rather than to the chloroplast degradation program, as their levels increase only in leaves undergoing developmental senescence, but not in dark-induced senescent leaves. Changes in the apoplastic space may be relevant for signaling and molecular trafficking underlying senescence.

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Section: Proteins That Accumulate In the Apoplast Of Senescent Leavesmentioning

confidence: 99%

Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

et al. 2020

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“…6). Most of them play critical roles in plant defense against biotic stress in previous reports [7,[31][32][33][34], and many were induced by E. ampelina infection in the HR grape at the earlier stages (Fig. 6).…”

Section: Transcription Factorsmentioning

confidence: 61%

Comparative transcriptome analysis of leaves from two grapevine species reveals an early gene expression profile associated with Elsinoe ampelina resistance

Min

Wan

Zhu

et al. 2020

Preprint

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Background: Anthracnose ( Elsinoe ampelina ) causes extensive damage to grapevine ( Vitis vinifera ) production worldwide, but the defense mechanisms exhibited by grape are not well understood. Results: In present study, the transcriptome differences of two grape species that exhibit either strong resistance (HR) or sensitivity (HS) to E . ampelina were determined at different time points up to 72 hours post infection (hpi) using RNA-seq profiling. Approximately 172 million high quality reads were obtained from a total of 40 samples. As a result, 3414 differentially expressed genes (DEGs) were identified, with 2,246 in the HR grape V . q uinquangularis Shang-24 accession and 2,019 in the HS grape V . vinifera Red Globe. More up-regulated than down-regulated genes were identified both in the HR and HS samples at each time point except 48 hours post infection. Conclusions: Gene ontology (GO) function and pathway enrichment analysis suggested that the grape transcriptional response to E . ampelina infection involves genes encoding protein kinases, transcription factors, metabolite synthesis, and phytohormone signaling. Although most of the GO functional categories and enriched pathways in response to E . ampelina infection were the same in the two species, the response was apparent much earlier in the HR grape (6 hours post infection and 24 hours post infection) than in the HS grape (48 hours post infection and 72 hours post infection), which may be associated with the contrasting resistance phenotypes. This study provides new insights into the grape defense system involved in responses to E . ampelina infection, and has identified several candidate genes that may be exploited in future biotechnological approaches to increase disease resistance in grapevine.

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“…Recently, miR398 was found to be involved in immunity against the blast fungus through regulating two genes encoding Cu/Zn-superoxidase dismutase (CSD) [35]. Besides, miR858-mediated regulation of phenylpropanoid biosynthetic pathway also played important role in Arabidopsis immunity [36]. These results demonstrated that certain interactions between lncRNAs and miRNAs may exist in melon, which provides a solid foundation for further investigation in the function of lncRNAs in PM tolerance.…”

Section: Discussionmentioning

confidence: 85%

Comparative transcriptome analysis uncovers regulatory roles of long non-coding RNAs involved in resistance to powdery mildew in melon

et al. 2020

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Background: Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs with more than 200 nucleotides in length, which play vital roles in a wide range of biological processes. Powdery mildew disease (PM) has become a major threat to the production of melon. To investigate the potential roles of lncRNAs in resisting to PM in melon, it is necessary to identify lncRNAs and uncover their molecular functions. In this study, we compared the lncRNAs between a resistant and a susceptible melon in response to PM infection. Results: It is reported that 11,612 lncRNAs were discovered, which were distributed across all 12 melon chromosomes, and > 85% were from intergenic regions. The melon lncRNAs have shorter transcript lengths and fewer exon numbers than protein-coding genes. In addition, a total of 407 and 611 lncRNAs were found to be differentially expressed after PM infection in PM-susceptible and PM-resistant melons, respectively. Furthermore, 1232 putative targets of differently expressed lncRNAs (DELs) were discovered and gene ontology enrichment (GO) analysis showed that these target genes were mainly enriched in stress-related terms. Consequently, co-expression patterns between LNC_018800 and CmWRKY21, LNC_018062 and MELO3C015771 (glutathione reductase coding gene), LNC_014937 and CmMLO5 were confirmed by qRT-PCR. Moreover, we also identified 24 lncRNAs that act as microRNA (miRNA) precursors, 43 lncRNAs as potential targets of 22 miRNA families and 13 lncRNAs as endogenous target mimics (eTMs) for 11 miRNAs. Conclusion: This study shows the first characterization of lncRNAs involved in PM resistance in melon and provides a starting point for further investigation into the functions and regulatory mechanisms of lncRNAs in the resistance to PM.

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MiR858-Mediated Regulation of Flavonoid-Specific MYB Transcription Factor Genes Controls Resistance to Pathogen Infection in Arabidopsis

Cited by 80 publications

References 55 publications

Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

Comparative transcriptome analysis of leaves from two grapevine species reveals an early gene expression profile associated with Elsinoe ampelina resistance

Comparative transcriptome analysis uncovers regulatory roles of long non-coding RNAs involved in resistance to powdery mildew in melon

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