miRNA-148b suppresses hepatic cancer stem cell by targeting neuropilin-1

Liu, Qinying; Xu, Yangmei; Wei, Shenghong; Gao, Wei; Chen, Li; Zhou, Tong; Wang, Zhen; Ying, Mingang; Zheng, Qinqin

doi:10.1042/bsr20150084

Cited by 55 publications

(59 citation statements)

References 36 publications

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“…Cell invasion assay was assessed using the Millipore 24‐well Millicell Chamber of pore size 8 μm (Millipore), according to Liu et al (). Briefly, the cells (2 × 10 5 ) in DMEM without FBS were added into the upper chamber pre‐coated with Matrigel (Sigma).…”

Section: Methodsmentioning

confidence: 99%

Lathyrus sativus diamine oxidase counteracts histamine‐induced cell proliferation, migration and pro‐angiogenic mediators release in human colon adenocarcinoma cell line Caco‐2

Pietrangeli

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Annunziata³

et al. 2019

Phytotherapy Research

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Because histamine is a modulator of cancer cell proliferation and invasiveness, this study aimed at investigating the effect of Lathyrus sativus–derived diamine oxidase (LSAO) and its mechanism of action on Caco‐2 cell line, considering that LSAO catalizes the oxidative deamination of histamine to the corresponding aldehyde, NH3 and H2O2. Histamine (0.01–1 μM) caused a proliferative effect on Caco‐2 cells promoting cell migration, invasion and nitric oxide and vascular endothelial growth factor release. Histamine (1 μM) stimulus also down regulated occludin expression, favouring up regulation of pro‐proliferative nuclear protein Ki67. Incubation with LSAO (0.004–0.4 μM) resulted in a significant inhibition of histamine‐induced effects. LSAO rescued occludin expression and down regulated Ki67, and it inhibited histamine‐induced increase of both MMP‐2 and 9 expression. Histamine effects were mediated by RhoA‐GTP down regulation and inversely related to phospho‐p38MAPK/p50/65 up regulation. These effects were counteracted by LSAO incubation. Histamine catabolism by LSAO accounts for a significant down regulation of proliferation and invasiveness of Caco‐2 cells. This study highlights the importance to control histamine levels in contrasting pro‐angiogenic and metastatization capability of colon cancer cells and expands the knowledge about the diamine oxidase from L. sativus seeding as a phytotherapeutic approach for colon cancer.

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Section: Methodsmentioning

confidence: 99%

Lathyrus sativus diamine oxidase counteracts histamine‐induced cell proliferation, migration and pro‐angiogenic mediators release in human colon adenocarcinoma cell line Caco‐2

Pietrangeli

Seguella

Annunziata³

et al. 2019

Phytotherapy Research

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“…miR-491 blocks the activation of NF-kB by regulating GIT-1 and inhibiting extracellular signal-regulated kinase (ERK), thereby reducing the stemness of HCSCs [26]. miR-148b restrains the formation of side population (SP) cells by regulating NRP1 and also helps regulate the proliferation, drug resistance, metastasis and angiogenesis of HCSCs [27]. In recent years, studies have shown that EMT plays an important role in tumor invasion and metastasis.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Role of MicroRNAs in Hepatic Cancer Stem Cells

Daia¹,

Zhang²

2017

Chemotherapy (Los Angel)

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Liver cancer is one of the most malignant tumors and is prone to relapse, metastasis and drug resistance. These phenomena can be explained by the existence of cancer stem cells (CSCs). CSCs have a strong ability to proliferate, are highly carcinogenic, exhibit multi-directional differentiation, develop drug resistance, and play critical roles in tumor radiotherapy, chemotherapy and tumor recurrence. miRNAs exert effects on oncogenes and tumor suppressor genes. There are distinctive miRNA expression profiles in different types of tumors, and these profiles are closely related to tumorigenesis, differentiation, metastasis and prognosis. Studies have shown that miRNAs are abnormally expressed in hepatocellular carcinoma and have important regulatory effects on the self-renewal and differentiation of hepatic cancer stem cells (HCSCs) as well as on the initiation of tumorigenesis. Therefore, it is critical to understand the impact of miRNAs in HCSC and the associated molecular mechanisms to develop new methods for the clinical diagnosis and treatment of liver cancer.

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“…The existence of HCC stem cells has been regarded as the immediate cause of clinical tumor recurrence and treatment failure for patients with HCC . Conventional ways of surgery, chemotherapy, and radiotherapy can kill a majority of carcinoma cells, but it is hard to eradicate cancer stem cells that maintain a high capacity for self‐renewal, chemoresistance, and pluripotency, leading to frequent recurrence or metastasis in patients with HCC . Therefore, there is an urgent need for new therapy strategies that could directly target HCC stem cells.…”

Section: Introductionmentioning

confidence: 99%

MiR‐490‐5p inhibits the stemness of hepatocellular carcinoma cells by targeting ECT2

Cai

et al. 2018

J of Cellular Biochemistry

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To explore the targeting relationship between miR-490-5p and ECT2 in hepatocellular carcinoma (HCC) and the influences of miR-490-5p and ECT2 on the stemness of HCC cells. The expressions of miR-490-5p and ECT2 in HCC tissues and adjacent tissues were identified by quantitative real-time polymerase chain reaction (qRT-PCR). The relationships between the expression levels of miR-490-5p/ ECT2 and the overall/disease-free survival (OS/DFS) of patients with HCC were evaluated using correlative curves. In addition, the targeting relationship between miR-490-5p and ECT2 was predicted by TargetScan and verified by dual-luciferase reporter assay. Plasmid transfection was used for overexpression of ECT2 in HepG2 cells, and transfection efficiency was verified by qRT-PCR. Cell Counting Kit-8 assay and cell sphere-formation assay were conducted to detect the proliferation and sphere-formation ability of HCC cells, respectively. Cell populations with different cell transfections were sorted using flow cytometry. The expression levels of proteins in the stem cell signaling pathway were determined using Western blot analysis. MiR-490-5p was remarkably downregulated, yet ECT2 was upregulated in HCC tissues compared with adjacent tissues. MiR-490-5p expression was positively correlated with OS and DFS of patients with HCC, which were otherwise negatively correlated with ECT2 expression. ECT2 was validated to be the downstream target of miR-490-5p. Overexpression of miR-490-5p restrained the sphere formation ability, stemness, and proliferation of HCC cells. MiR-490-5p repressed the stemness of HCC cells through inhibiting the expression of ECT2. MiR-490-5p may be an underlying therapeutic target in HCC treatment.

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miRNA-148b suppresses hepatic cancer stem cell by targeting neuropilin-1

Cited by 55 publications

References 36 publications

Lathyrus sativus diamine oxidase counteracts histamine‐induced cell proliferation, migration and pro‐angiogenic mediators release in human colon adenocarcinoma cell line Caco‐2

Lathyrus sativus diamine oxidase counteracts histamine‐induced cell proliferation, migration and pro‐angiogenic mediators release in human colon adenocarcinoma cell line Caco‐2

Characterization of the Role of MicroRNAs in Hepatic Cancer Stem Cells

MiR‐490‐5p inhibits the stemness of hepatocellular carcinoma cells by targeting ECT2

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