miRNA analysis of prostate cancer by quantitative real time PCR: Comparison between formalin-fixed paraffin embedded and fresh-frozen tissue

Contribution of microRNA analysis to characterisation of pancreatic lesions: a review

“…Due to their short length, the mature miRNAs seem not to be influenced by nucleic acid degradation caused by formalin fixation,32 as happened on the contrary for long RNA or DNA. Several papers reported the feasibility of miRNA expression from FFPE specimens in different tissues as kidney,33 prostate,34 35 breast36 and brain 37. Also for pancreatic lesions, several papers have analysed miRNA expression profile in FFPE specimens compared with non-neoplastic or benign tissue, obtaining robust and reliable results 28 30 38–58…”

“…The most frequent method used for assessing miRNA expression is qRT-PCR 12 13 15 18–21 27 30 31 35 40 41 43 44 49 50 53 55 56 58 61 66 67 70–74 76–80. Several approaches to analyse miRNAs continue to be based on single-gene assays; however, high-throughput technologies can be employed to identify differences in miRNA expression levels between normal and cancerous tissues.…”

Micro-RNA – A potential for forensic science?

“…This also applies for formalin-fixed paraffineembedded (FFPE) tissues where intense nucleic acid fractionation occurs and which can be of pivotal importance to forensic casework. Not only has miRNA recovery from FFPE tissues been shown to be feasible and to lead to valid results [124][125][126] but it also outperforms FFPE tissue mRNA expression profiling by achieving a higher degree of resemblance to fresh tissue profiles and therefore significantly better correlation [127].…”

Developing a Prognostic Micro-RNA Signature for Human Cervical Carcinoma

“…This experience would appear to contradict several previous studies that have reported high concordance in the miRNA expression data from tissue-matched frozen and FFPE samples derived from various types of human tissues, including: breast [4], lung [42], kidney [43], skin [44], glioblastoma [45], melanoma [46, 47], prostate [48, 49], and lymph nodes [50]. However, with the exception of our own single study that used the TLDA platform [4], the remaining reports utilized other miRNA profiling technologies, such as microarray [42, 44, 46, 47, 50], deep sequencing [42, 43], custom PCR array [48], and qRT-PCR using stem-loop [49] or locked-nucleic-acid primers [45]. There has only been one report to date that evaluated miRNA expression data from tissue-matched frozen and FFPE cervix samples, which only analyzed 3 cervix specimens, in addition to 3 breast and 2 gall bladder samples [51].…”