miRNA biogenesis: Biological impact in the development of cancer

Romero-Córdoba, Sandra; Salido-Guadarrama, Iván; Rodríguez-Dorantes, Mauricio; Hidalgo-Miranda, Alfredo

doi:10.4161/15384047.2014.955442

Cited by 214 publications

(153 citation statements)

References 150 publications

(122 reference statements)

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“…2B and D). In addition, knockdown of Dicer, the ribonuclease that cleaves the miRNA precursor for the biogenesis of mature miRNA [35], increased the MMP-14 levels and abolished the effects of miR-584-5p precursor on the MMP-14 expression in NB cells ( Supplementary Fig. S2A and B), which was in line with the inverse correlation between Dicer and MMP-14 expression in public NB datasets ( Supplementary Fig.…”

Section: Mir-584-5p Represses the Transcription Of Mmp-14 In Nb Cellssupporting

confidence: 80%

miRNA-584-5p exerts tumor suppressive functions in human neuroblastoma through repressing transcription of matrix metalloproteinase 14

Xuan

Hong

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Matrix metalloproteinase 14 (MMP-14) is a membrane-anchored MMP crucial for tumorigenesis and aggressiveness, and is highly expressed in neuroblastoma (NB), the most common extracranial solid tumor in childhood. Recent evidence shows the emerging roles of endogenous promoter-targeting microRNAs (miRNAs) in regulating gene transcription. However, the roles of miRNAs in the transcription of MMP-14 still remain largely unknown. In this study, through mining computational algorithm program and Argonaute-chromosome interaction dataset, we identified one binding site of miRNA-584-5p (miR-584-5p) within the MMP-14 promoter. In NB tissues, miR-584-5p was under-expressed and inversely correlated with MMP-14 expression, and was an independent prognostic factor for favorable outcome of patients. miR-584-5p precursor attenuated the expression of MMP-14 in a Dicer-dependent manner, resulting in decreased levels of vascular endothelial growth factor, in cultured NB cell lines. In addition, miR-584-5p suppressed the promoter activity of MMP-14, and mutation of miR-584-5p binding site abolished these effects. Mechanistically, miR-584-5p recruited Argonaute 2 to facilitate the enrichment of enhancer of zeste homolog 2, histone H3 lysine 27 trimethylation, and histone H3 lysine 9 dimethylation on MMP-14 promoter in NB cells, which was abolished by repressing the miR-584-5p-promoter interaction. Gain- and loss-of-function studies demonstrated that miR-584-5p suppressed the growth, invasion, metastasis, and angiogenesis of NB cells in vitro and in vivo. Moreover, restoration of MMP-14 expression rescued the NB cells from changes in these biological features. Taken together, these results indicate that promoter-targeting miR-584-5p exerts tumor suppressive functions in NB through repressing the transcription of MMP-14.

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Section: Mir-584-5p Represses the Transcription Of Mmp-14 In Nb Cellssupporting

confidence: 80%

miRNA-584-5p exerts tumor suppressive functions in human neuroblastoma through repressing transcription of matrix metalloproteinase 14

Xuan

Hong

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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show abstract

“…They can bind to 3′ untranslated regions (3′UTR) of the mRNAs transcribed from their target genes, and therefore led to mRNA degradation or repression of protein translation 6. The dysregulation of miRNAs has been confirmed to be strongly related with tumorigenesis, and miRNAs work as tumour promoters or suppressors due to regulation of different target genes 7. Glioma is no exception.…”

Section: Introductionmentioning

confidence: 99%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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This study was designed to explore the relationship between miR‐1275 and SERPINE1 and its effects on glioma cell proliferation, migration, invasion and apoptosis. Differentially expressed miRNAs and mRNAs in glioma tissues were screened out by bioinformatic analysis. Dual‐luciferase reporter gene assay was used to validate the targeted relationship between miR‐1275 and SERPINE1. qRT‐PCR was used to detect the expression of miR‐1275 and SERPINE1 in glioma tissues. The expressions of SERPINE1 and p53 pathway‐related proteins in glioma cells were detected by western blot. Glioma cell proliferation, apoptosis, migration and invasion were respectively detected by CCK‐8 assay, flow cytometry, wound healing assay and transwell assay. Tumour xenograft model was developed to study the influence of miR‐1275 and SERPINE1 on glioma growth in vivo. The results of microarray analysis, qRT‐PCR and western blot showed that miR‐1275 was low‐expressed while SERPINE1 was high‐expressed in glioma. Dual‐luciferase assay showed that miR‐1275 could bind to SERPINE1. Overexpression of miR‐1275 could promote the p53 pathway‐related proteins’ expression. Highly expressed miR‐1275 could repress the migration, proliferation and invasion of glioma cells while highly expressed SERPINE1 had inverse effects. Tumour xenograft showed that up‐regulated miR‐1275 or down‐regulated SERPINE1 could repress glioma growth in vivo. Up‐regulation of miR‐1275 activated p53 signalling pathway via regulating SERPINE1 and therefore suppressed glioma cell proliferation, invasion and migration, whereas promoted cell apoptosis.

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“…MicroRNAs (miRNAs or miRs) are a recently discovered class of small (19)(20)(21)(22)(23)(24) nucleotides) endogenous non-coding oligonucleotides that regulate gene expression at the post-transcriptional level [1][2][3]. They play a crucial role in regulation of physiological and pathological processes and some miRNAs have been described as associated with distinct clinical characteristics, such as diagnosis or disease activity [4][5][6][7][8][9]. For these reasons, miRNAs are attractive as potential biomarkers for the diagnosis, prognosis, disease activity and severity of various diseases.…”

Section: Introductionmentioning

confidence: 99%

Could MicroRNA polymorphisms influence warfarin dosing? A pharmacogenetics study on mir133 genes

Ciccacci

Rufini

Politi

et al. 2015

Thrombosis Research

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miRNA biogenesis: Biological impact in the development of cancer

Cited by 214 publications

References 150 publications

miRNA-584-5p exerts tumor suppressive functions in human neuroblastoma through repressing transcription of matrix metalloproteinase 14

miRNA-584-5p exerts tumor suppressive functions in human neuroblastoma through repressing transcription of matrix metalloproteinase 14

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Could MicroRNA polymorphisms influence warfarin dosing? A pharmacogenetics study on mir133 genes

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