miRNA genetic variants: As potential diagnostic biomarkers for oral cancer

Tandon, Divya; Dewangan, Jayant; Srivastava, Sonal; Garg, Vivek Kumar; Rath, Srikanta Kumar

doi:10.1016/j.prp.2017.10.002

Cited by 14 publications

(8 citation statements)

References 29 publications

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“…A polymorphism of rs3746444 in the miR‐499 gene has been implicated in tumorigenesis through regulating the expression level of the SOX gene. Numerous studies have reported that SOX‐6 expression was deregulated in ESCC, oral squamous cell cancer, and pancreatic cancer 45,47,48 . A recent meta‐analysis demonstrated that a SNP of rs3746444 significantly increased DSC risk, 31 which was consistent with our result that the rs3746444 A to G variation could increase the risk of ESCC.…”

Section: Discussionsupporting

confidence: 91%

Association between miR‐146a rs2910164, miR‐196a2 rs11614913, and miR‐499 rs3746444 polymorphisms and the risk of esophageal carcinoma: A case–control study

et al. 2022

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Esophageal cancer is a major public health problem and social burden in China, and it is the fourth leading cause of cancer death with a 8.04% mortality rate in 2015. 1 There are two main types of esophagus cancer: esophageal squamous cell cancer (ESCC) and esophageal adenocarcinoma (EAC). ESCC accounts for more than 90% of the cases in China 2 ; however, since the 1970s, the incidence of EAC has significantly increased

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Section: Discussionsupporting

confidence: 91%

Association between miR‐146a rs2910164, miR‐196a2 rs11614913, and miR‐499 rs3746444 polymorphisms and the risk of esophageal carcinoma: A case–control study

et al. 2022

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“…Similarly, research on tongue squamous cell carcinoma displayed downregulation of this miRNA in tumor tissue [61]. Interestingly, in oral cancer, the expression of miR-149-5p was significantly upregulated in tumor tissue compared with peripheral control tissue [62]. Our results showed that expression of miR-149-5p was significantly downregulated in oral tumor tissue, but no difference was observed in laryngeal cancer.…”

Section: Discussionsupporting

confidence: 57%

miRNAs as Biomarkers for Diagnosing and Predicting Survival of Head and Neck Squamous Cell Carcinoma Patients

Piotrowski

Zhu

Saccon

et al. 2021

Cancers

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Head and Neck Squamous Cell Carcinoma (HNSCC) is the sixth most common cancer worldwide. These tumors originate from epithelial cells of the upper aerodigestive tract. HNSCC tumors in different regions can have significantly different molecular characteristics. While many microRNAs (miRNAs) have been found to be involved in the regulation of the carcinogenesis and pathogenesis of HNSCC, new HNSCC related miRNAs are still being discovered. The aim of this study was to explore potential miRNA biomarkers that can be used to diagnose HNSCC and prognose survival of HNSCC patients. For this purpose, we chose a panel of 12 miRNAs: miR-146a-5p, miR-449a, miR-126-5p, miR-34a-5p, miR-34b-5p, miR-34c-5p, miR-217-5p, miR-378c, miR-6510-3p, miR-96-5p, miR-149-5p, and miR-133a-5p. Expression of these miRNAs was measured in tumor tissue and neighboring healthy tissue collected from patients diagnosed with HNSCC (n = 79) in either the oral cavity, oropharynx, or larynx. We observed a pattern of differentially expressed miRNAs at each of these cancer locations. Our study showed that some of these miRNAs, separately or in combination, could serve as biomarkers distinguishing between healthy and tumor tissue, and their expression correlated with patients’ overall survival.

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“…This is attested by numerous recent studies that have used PCR-RFLP methodologies both in research and as a routine molecular diagnostic tool. For instance, CAPS assays were successfully developed as potential biomarkers for oral cancer [47], for contributing to a paradigm shift and clearly establishing male heterogamy in two henophidian snakes [48], for molecular sexing in the endangered Galapagos petrel [49] and for ascertaining the identity of the amoebiasis-causing Entamoeba histolytica [50]. The same approach was employed for the rapid diagnosis of neonatal sepsis in India [51] and to determine the magnitude of drug resistance in Mycobacterium tuberculosis in Central Punjab, Pakistan [52].…”

Section: Discussionmentioning

confidence: 99%

Derived Polymorphic Amplified Cleaved Sequence (dPACS): A Novel PCR-RFLP Procedure for Detecting Known Single Nucleotide and Deletion–Insertion Polymorphisms

Kaundun

Marchegiani

Hutchings

et al. 2019

IJMS

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Most methods developed for detecting known single nucleotide polymorphisms (SNP) and deletion–insertion polymorphisms (DIP) are dependent on sequence conservation around the SNP/DIP and are therefore not suitable for application to heterogeneous organisms. Here we describe a novel, versatile and simple PCR-RFLP procedure baptised ‘derived Polymorphic Amplified Cleaved Sequence’ (dPACS) for genotyping individual samples. The notable advantage of the method is that it employs a pair of primers that cover the entire fragment to be amplified except for one or few diagnostic bases around the SNP/DIP being investigated. As such, it provides greater opportunities to introduce mismatches in one or both of the 35–55 bp primers for creating a restriction site that unambiguously differentiates wild from mutant sequences following PCR-RFLP and horizontal MetaPhorTM gel electrophoresis. Selection of effective restriction enzymes and primers is aided by the newly developed dPACS 1.0 software. The highly transferable dPACS procedure is exemplified here with the positive detection (in up to 24 grass and broadleaf species tested) of wild type proline106 of 5-enolpyruvylshikimate-3-phosphate synthase and its serine, threonine and alanine variants that confer resistance to glyphosate, and serine264 and isoleucine2041 which are key target-site determinants for weed sensitivities to some photosystem II and acetyl-CoA carboxylase inhibiting herbicides, respectively.

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miRNA genetic variants: As potential diagnostic biomarkers for oral cancer

Cited by 14 publications

References 29 publications

Association between miR‐146a rs2910164, miR‐196a2 rs11614913, and miR‐499 rs3746444 polymorphisms and the risk of esophageal carcinoma: A case–control study

Association between miR‐146a rs2910164, miR‐196a2 rs11614913, and miR‐499 rs3746444 polymorphisms and the risk of esophageal carcinoma: A case–control study

miRNAs as Biomarkers for Diagnosing and Predicting Survival of Head and Neck Squamous Cell Carcinoma Patients

Derived Polymorphic Amplified Cleaved Sequence (dPACS): A Novel PCR-RFLP Procedure for Detecting Known Single Nucleotide and Deletion–Insertion Polymorphisms

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