miRNA Profiling of Major Testicular Germ Cells Identifies Stage-Specific Regulators of Spermatogenesis

Sethi, Shruti; Mehta, Poonam; Pandey, Aastha; Gupta, Gopal; Rajender, Singh

doi:10.1007/s43032-022-01005-x

Cited by 5 publications

(4 citation statements)

References 91 publications

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“…Sethi et al. ( 108 ) identified differentially expressed miRNAs among pachytene spermatocytes, round spermatids, and mature rat sperm using high-throughput transcriptomic sequencing. A total of 149 miRNAs were restricted to primary spermatocytes as they were not detected in the post-meiotic cells.…”

Section: Mechanism Of Mirna Involved In Spermatogenesismentioning

confidence: 99%

“…Additionally, various processes during meiotic prophase I, such as synapsis and crossover formation, are disturbed, and intersister chromatid synapses have been observed (79). Sethi et al (108) identified differentially expressed miRNAs among pachytene spermatocytes, round spermatids, and mature rat sperm using highthroughput transcriptomic sequencing. A total of 149 miRNAs were restricted to primary spermatocytes as they were not detected in the post-meiotic cells.…”

Section: Mirnas and Spermatocytes In Meiosismentioning

confidence: 99%

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MicroRNAs in spermatogenesis dysfunction and male infertility: clinical phenotypes, mechanisms and potential diagnostic biomarkers

Shi,

Yu,

Guo

et al. 2024

Front. Endocrinol.

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Infertility affects approximately 10–15% of couples worldwide who are attempting to conceive, with male infertility accounting for 50% of infertility cases. Male infertility is related to various factors such as hormone imbalance, urogenital diseases, environmental factors, and genetic factors. Owing to its relationship with genetic factors, male infertility cannot be diagnosed through routine examination in most cases, and is clinically called ‘idiopathic male infertility.’ Recent studies have provided evidence that microRNAs (miRNAs) are expressed in a cell-or stage-specific manner during spermatogenesis. This review focuses on the role of miRNAs in male infertility and spermatogenesis. Data were collected from published studies that investigated the effects of miRNAs on spermatogenesis, sperm quality and quantity, fertilization, embryo development, and assisted reproductive technology (ART) outcomes. Based on the findings of these studies, we summarize the targets of miRNAs and the resulting functional effects that occur due to changes in miRNA expression at various stages of spermatogenesis, including undifferentiated and differentiating spermatogonia, spermatocytes, spermatids, and Sertoli cells (SCs). In addition, we discuss potential markers for diagnosing male infertility and predicting the varicocele grade, surgical outcomes, ART outcomes, and sperm retrieval rates in patients with non-obstructive azoospermia (NOA).

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Section: Mechanism Of Mirna Involved In Spermatogenesismentioning

confidence: 99%

Section: Mirnas and Spermatocytes In Meiosismentioning

confidence: 99%

MicroRNAs in spermatogenesis dysfunction and male infertility: clinical phenotypes, mechanisms and potential diagnostic biomarkers

Shi,

Yu,

Guo

et al. 2024

Front. Endocrinol.

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“…Spermatogenesis in male mammals has three phases: mitotic proliferation of spermatogonia, meiosis of spermatocytes, and haploid differentiation of spermatids. The genome of male germ cells is actively transcribed to generate a complex transcriptome with a specific expression pattern in every spermatogenesis phase [ 149 ]. According to the importance of the precise regulation needed for successful sperm production, the role of miRNAs has been highlighted particularly for their post-translation effects on stable mRNA expression [ 150 ].…”

Section: The Role In Spermatogenesismentioning

confidence: 99%

Overview of miR-106a Regulatory Roles: from Cancer to Aging

Daneshpour

Ghadimi-Daresajini

2023

Bioengineering

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MicroRNAs (miRNAs) comprise a class of non-coding RNA with extensive regulatory functions within cells. MiR-106a is recognized for its super-regulatory roles in vital processes. Hence, the analysis of its expression in association with diseases has attracted considerable attention for molecular diagnosis and drug development. Numerous studies have investigated miR-106 target genes and shown that this miRNA regulates the expression of some critical cell cycle and apoptosis factors, suggesting miR-106a as an ideal diagnostic and prognostic biomarker with therapeutic potential. Furthermore, the reported correlation between miR-106a expression level and cancer drug resistance has demonstrated the complexity of its functions within different tissues. In this study, we have conducted a comprehensive review on the expression levels of miR-106a in various cancers and other diseases, emphasizing its target genes. The promising findings surrounding miR-106a suggest its potential as a valuable biomolecule. However, further validation assessments and overcoming existing limitations are crucial steps before its clinical implementation can be realized.

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“…Studies on testicular germ cells have shown that small RNAs undergo significant changes in their level and patterns of expression during spermatogenic development of the germ cells [ 13 ]. Germ cells (spermatocytes, round spermatids and spermatozoa) share a large number of small non-coding RNAs apart from having a set of RNAs unique to each cell type [ 14 ]. This suggested that the regulation of spermatogenesis is more complicated than understood till now.…”

Section: Introductionmentioning

confidence: 99%

Small RNAs, spermatogenesis, and male infertility: a decade of retrospect

Joshi,

Sethi,

Mehta

et al. 2023

Reprod Biol Endocrinol

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Small non-coding RNAs (sncRNAs), being the top regulators of gene expression, have been thoroughly studied in various biological systems, including the testis. Research over the last decade has generated significant evidence in support of the crucial roles of sncRNAs in male reproduction, particularly in the maintenance of primordial germ cells, meiosis, spermiogenesis, sperm fertility, and early post-fertilization development. The most commonly studied small RNAs in spermatogenesis are microRNAs (miRNAs), PIWI-interacting RNA (piRNA), small interfering RNA (siRNA), and transfer RNA-derived small RNAs (ts-RNAs). Small non-coding RNAs are crucial in regulating the dynamic, spatial, and temporal gene expression profiles in developing germ cells. A number of small RNAs, particularly miRNAs and tsRNAs, are loaded on spermatozoa during their epididymal maturation. With regard to their roles in fertility, miRNAs have been studied most often, followed by piRNAs and tsRNAs. Dysregulation of more than 100 miRNAs has been shown to correlate with infertility. piRNA and tsRNA dysregulations in infertility have been studied in only 3–5 studies. Sperm-borne small RNAs hold great potential to act as biomarkers of sperm quality and fertility. In this article, we review the role of small RNAs in spermatogenesis, their association with infertility, and their potential as biomarkers of sperm quality and fertility.

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miRNA Profiling of Major Testicular Germ Cells Identifies Stage-Specific Regulators of Spermatogenesis

Cited by 5 publications

References 91 publications

MicroRNAs in spermatogenesis dysfunction and male infertility: clinical phenotypes, mechanisms and potential diagnostic biomarkers

MicroRNAs in spermatogenesis dysfunction and male infertility: clinical phenotypes, mechanisms and potential diagnostic biomarkers

Overview of miR-106a Regulatory Roles: from Cancer to Aging

Small RNAs, spermatogenesis, and male infertility: a decade of retrospect

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