Mirocrystalline collagen as a biologic dressing, vascular prosthesis, and hemostatic agent

Hait, M R; Battista, O A; Stark, Richard B.; McCord, Colin

doi:10.1097/00006534-197005000-00057

Cited by 10 publications

(11 citation statements)

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“…5 Microfibrillar collagen was initially reported to have hemostatic properties in 1969. 19 It is a parted hydrochloric acid amino salt of natural collagen with microcrystals 1 /z,m long. The microfibrillar collagen we used is produced from bovine dermal collagen.…”

Section: Discussionmentioning

confidence: 99%

Microfibrillar collagen model of canine cerebral infarction.

Purdy

Devous

Batjer

et al. 1989

Stroke

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A new canine model of focal cerebral ischemia has been developed employing intravascular delivery of microfibrillar collagen via femoral catheterization. In 13 dogs, dose-effect studies showed neurologic deficits (ranging from mild hemiparesis to death) related to the dose of microfibrillar collagen delivered. In another 10 dogs, 0.5 ml of 60 mg/ml microfibrillar collagen was injected into the common carotid artery; neurologic assessment over 48 hours revealed a survivable stroke syndrome in seven dogs, death at 40 hours in one dog and at <12 hours in another, and no clinical effect in one dog. The eight surviving dogs were sacrificed at 48 hours; nine of the 10 dogs had middle cerebral artery distribution infarcts (two grossly hemorrhagic and five grossly nonhemorrhagic) on histologic examination. Angiography in three dogs demonstrated no significant major vascular occlusion. All seven dogs with survivable strokes demonstrated a dense hemiparesis at 24 hours that improved to ambulatory status at 48 hours. O ne of the most widely used animal models of focal ischemia involves permanent or reversible occlusion of the middle cerebral artery (MCA). -11 However, this model is compromised by the necessity of enucleation to gain access to the MCA, by violation of the dura, and by the need for sophisticated microsurgery. The physical manipulation of subarachnoid vessels and adjacent brain tissue as well as the risk of spinal fluid leakage further complicates this model. The fact that intracranial surgery is required raises questions as to whether the model is sufficiently analogous to embolic disease to allow extrapolation of physiologic data to the more common circumstance in humans. One embolic model in dogs 5 -6 ' 11 uses silicone cylinders introduced into the internal carotid artery to embolize the MCA, producing basal ganglia infarcts. However, this technique Received September 21, 1988; accepted March 17, 1989. also requires surgical access to the carotid arteries in the neck and results in persistent occlusion of the main trunk of the MCA. We have recently developed a modification of this model that results in reversible occlusion. 11 Primates would provide the best model for cerebrovascular disease amenable to study with noninvasive imaging modalities; however, both ethical and economic issues limit the widespread use of primates.Because their brains are large enough to image radiographically, dogs represent an acceptable alternative to primates as candidates for the study of cerebrovascular disease. Remote delivery of embolic material is desirable to study ischemia immediately following its onset without contamination by surgical artifacts. Using microfibrillar collagen (Avitene; Aicon Laboratories, Inc., Fort Worth, Texas), we have developed a model for cerebral ischemia in dogs that consistently creates infarcts in the MCA distribution and permits clinical-pathologic correlations and simultaneous physiologic studies. We describe the model and the attendant clinical, angiographic, and histopathologic result...

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Section: Discussionmentioning

confidence: 99%

Microfibrillar collagen model of canine cerebral infarction.

Purdy

Devous

Batjer

et al. 1989

Stroke

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“…Earlier studies had shown MFC to be an excellent hemostatic agent. [23][24][25] In 1969, Hait et al 26 demonstrated the hemostatic properties of MFC in hepatic and splenic lacerations in mongrel dogs. Microscopic examination showed that MFC adhered to the parenchyma and triggered plateCorrespondence to: D. Orgill let aggregation.…”

Section: Introductionmentioning

confidence: 99%

Polyethylene glycol/microfibrillar collagen composite as a new resorbable hemostatic bone wax

Orgill

Ehret

Regan

et al. 1998

J. Biomed. Mater. Res.

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Although bone wax is effective at achieving hemostasis, it is nonresorbable, causes a foreign body reaction, and inhibits osteogenesis. We report development of a polyethylene glycol/microfibrillar collagen composite (PEG/MFC) that has inherent hemostatic qualities, is biodegradable, and is compatible with bone repair. PEG/MFC composite (n = 42) was placed in 5 mm cranial defects in New Zealand white rabbits. Hemostasis and healing were compared to unfilled defects (n = 32) and defects filled with standard bone wax (n = 10). Both PEG/MFC and bone wax handled well and stopped bleeding. The polyethylene glycol component was resorbed by 8 h, and the microfibrillar collagen was resorbed over 2 months, eliciting only a minor inflammatory response during the first month. Defects filled with the PEG/MFC composite showed similar amounts of bony regeneration as did unfilled control defects. At 4 weeks, healing bone accounted for 43 +/- 13% in those treated with PEG/MFC and 47 +/- 19% defect area in untreated holes. In contrast, less than 1% of the area was bone in defects filled with bone wax (p < 0.05). PEG/MFC composite provided excellent bony hemostasis and did not inhibit bone growth.

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“…The limited availability of human skin stimulated investigations of other suitable sources of biological dressings, such as animal skin [12,16,19], amnionic membranes [11], human fetal skin [1], and bovine collagen, reconstituted on a dacron mesh [7,17]. The following report compares the effects of autografts with a bovine collagen preparation as a dressing on fresh, contaminated surgical wounds in rats.…”

Section: Introductionmentioning

confidence: 99%

The effect of a collagen dressing on contaminated surgical wounds in rats

Burget

Nathan

Holder

et al. 1976

Langenbecks Arch Chiv

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In maintaining the rat, the collagen dressings, Aviderm Amine and Aviderm, are as effective as skin in low bacterial levels in contaminated surgical wounds. In the scald-burn wound in the rat the effect of skin dressing is confirmed. Also, 48 h after collagen dressing in the wound the bacterial contamination was the same as that seen under autograft dressings. We have elsewhere suggested that skin and some other dressings modify the local host defense by their effect on the accumulated white blood cells. We believe that collagen, as well as some synthetics, can alter the local environment permitting the destruction of invading bacteria by the accumulated white blood cells.

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Mirocrystalline collagen as a biologic dressing, vascular prosthesis, and hemostatic agent

Cited by 10 publications

References 0 publications

Microfibrillar collagen model of canine cerebral infarction.

Microfibrillar collagen model of canine cerebral infarction.

Polyethylene glycol/microfibrillar collagen composite as a new resorbable hemostatic bone wax

The effect of a collagen dressing on contaminated surgical wounds in rats

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