MIRU-VNTR typing adds discriminatory value to groups of Mycobacterium bovis and Mycobacterium caprae strains defined by spoligotyping

Duarte, E.L.; Domingos, M.; Amado, A.; Cunha, Mónica V.; Botelho, Ana

doi:10.1016/j.vetmic.2009.11.027

Cited by 46 publications

(57 citation statements)

References 18 publications

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“…This finding is in agreement with previously published data showing that MIRU locus 26 is the most discriminatory locus for M. bovis, while loci 2, 4,10,20,23,24,27,31,39 and 40 are less or not at all discriminatory (Roring et al 2004). Contrary to these findings and other reports, some studies observed little allelic diversity for MIRU locus 16 (Roring et al 2004, Boniotti et al 2009, Duarte et al 2010). In addition, Hilty et al (2005) found a moderate level of allelic polymorphism for MIRU loci 4 and 27 and Allix et al (2006) reported high allelic diversity for MIRU loci 24 and 27.…”

Section: Discussioncontrasting

confidence: 81%

“…In the present study, we applied two molecular fingerprinting methods, spoligotyping and MIRU-VNTR typing, to assess the genotypic diversity of Brazilian strains of M. bovis and to better understand bovine TB transmission in Brazil. Previous studies already demonstrated the efficiency of both methods in several countries (Sola et al 2003, Boniotti et al 2009, Duarte et al 2010. In Brazil, however, IS6110-RFLP, polymorphic guanine-cytosine-rich sequence-RFLP and spoligotyping, but not MIRU-VNTR typing, have been reported as typing methods for M. bovis isolates (Zanini et al 2001, Rodriguez et al 2004).…”

Section: Discussionmentioning

confidence: 99%

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Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil

Parreiras

Andrade

Nascimento

et al. 2012

Mem. Inst. Oswaldo Cruz

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MIRU-VNTR typing produced 16 distinct genotypes, with 53 isolates forming eight of the groups, and individual isolates with unique VNTR profiles forming the remaining eight groups. The allelic diversity of each VNTR locus was calculated and only two of the 12-MIRU-VNTR loci presented scores with either a moderate (0.4, MIRU16) or high (0.6, MIRU26) discriminatory index (h). Both typing methods produced similar discriminatory indexes (spoligotyping h = 0.85; MIRU-VNTR h = 0.86) and the combination of the two methods increased the h value to 0.94, resulting in 29 distinct patterns. These results confirm that spoligotyping and VNTR analysis are valuable tools for studying the molecular epidemiology of M. bovis infections in Brazil. Key words: Mycobacterium bovis -spoligotyping -MIRU-VNTR typing -bovine tuberculosis -molecular epidemiologyGenotyping of M. bovis from Brazil • Patrícia Martins Parreiras et al. 65While IS6110-RFLP lacks resolution in this species, spoligotyping has been demonstrated to be a fast and cost-effective method for first-line typing (Haddad et al. 2004). The MIRU-VNTR typing procedure has been extensively evaluated in M. tuberculosis and the recent data available about this method on M. bovis strains confirmed its considerable power of discrimination (Sola et al. 2003, Michel et al. 2008, 2010. Smith et al. (2006) inferred that a combination of spoligotyping and VNTR typing resulted in the simplest and most cost-effective method for routine molecular typing and epidemiological tracing of bovine TB in Great Britain.Some previous studies have successfully employed techniques such as IS6110-RFLP and spoligotyping to genotype M. bovis strains isolated from cattle in Brazil (Zanini et al. 2001, Rodriguez et al. 2004), but as far as we know, no data exist on genotyping of M. bovis by combining spoligotyping and MIRU-VNTR typing in our country. We aimed to use spoligotyping and MIRU-VNTR typing to assess the genetic diversity of Brazilian M. bovis isolates. MATERIALS AND METHODS Sampling and conventional procedures -During 2001and 2002, tissue samples from cattle with lesions suggestive of TB were collected immediately after slaughter at the time of inspection in abattoirs. The study samples were collected in abattoirs that followed slaughter inspection procedures from the states of Amazonas (AM), Paraíba (PB), Distrito Federal (DF), Mato Grosso do Sul (MS), São Paulo, Santa Catarina (SC) and Minas Gerais (MG). The sampled specimens from respective carcasses were pooled, put on ice and sent to the Laboratório Nacional Agropecuário de Minas Gerais (Pedro Leopoldo, MG), where they were processed for inoculation in Stonebrink culture medium (Mota 1985). The cultures were incubated at 37ºC and verified for bacterial growth every week for a period of at least two months. Bacterial isolates were submitted to standard procedures for differentiation of certain species of the MTBC, including the evaluation of niacin production, nitrate reductase activity, Tween hydrolysis, catalase activity at 68ºC and resis...

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Section: Discussioncontrasting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil

Parreiras

Andrade

Nascimento

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…There was no variation at loci ETR-D and QUB11b. Locus QUB3232 was the most discriminatory locus, followed by ETR-B, which is consistent with other studies (1,7,9). Loci QUB3232 and QUB11a have been previously found to have a higher mutation rate regarding allelic diversity in M. tuberculosis and were therefore considered hypervariable (27); nevertheless, their use remains controversial as they may contribute to improved discrimination in determined settings (15).…”

supporting

confidence: 88%

Limitations of Spoligotyping and Variable-Number Tandem-Repeat Typing for Molecular Tracing of Mycobacterium bovis in a High-Diversity Setting

et al. 2011

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“…Strains with the same spoligotype are assumed to be individuals recently derived by clonal replication from a single ancestral cell; therefore, epidemiological related strains should have higher genetic similarity than those no related (Rodríguez-Campos et al, 2011;Milián et al, 2016). Furthermore, spoligotyping has been used successfully in epidemiological studies in many countries (Gibson et al, 2004;Parra et al, 2005;Duarte et al, 2010;Rodríguez et al, 2010;Skuce et al, 2010;Ruettger et al, 2012;Mwakapuja et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Mycobacterium bovis in Jalisco, Mexico: Tracing back sources of infection

GonzÃ¡lez-Ruiz¹,

Sosa-Gallegos²,

RodrÃguez-HernÃ¡ndez³

et al. 2018

J. Vet. Med. Anim. Health

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Bovine tuberculosis (bTB) is a disease of cattle that presents risk to public health, causing severe economic losses to the livestock industry and difficulty in eradication because of its complex epidemiology. The aim of this study was to identify relationships between Mycobacterium bovis strains from cattle in the State of Jalisco, and those of other States of México. Molecular fingerprints of 337 M. bovis isolates from Jalisco, and 1152 from other States of México were included in the study. Isolates were obtained from tubercles between 1997 and 2015. Evolutionary relationship was determined throughout spoligoforest (www.emi.unsw.edu.au/spoltools/). From 337 isolates from Jalisco, 59 spoligotypes were obtained, ten of them included 48% of all isolates in the state. Five spoligotypes were common to beef and dairy cattle. The molecular analysis showed eight clusters in a philogenetic three: one with three subclusters of nine isolates each, all from dairy cattle; four with two isolates, including dairy and beef cattle. All spoligotypes from Jalisco have been reported in other states, four of the most frequent ones: SB0673, SB0971, SB0669 and SB0140, were the same as in other states. The most frequent spoligotypes of M. bovis found in Jalisco were also the most frequent ones in other parts of Mexico. However, there is no evidence to conclude that Jalisco is the source of infection to other states since no information on movement and destination of cattle could be documented.

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MIRU-VNTR typing adds discriminatory value to groups of Mycobacterium bovis and Mycobacterium caprae strains defined by spoligotyping

Cited by 46 publications

References 18 publications

Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil

Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil

Limitations of Spoligotyping and Variable-Number Tandem-Repeat Typing for Molecular Tracing of Mycobacterium bovis in a High-Diversity Setting

Genetic diversity of Mycobacterium bovis in Jalisco, Mexico: Tracing back sources of infection

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