Miscibility of poly(acrylic acid)/poly(methyl vinyl ketone) blend and in vitro application as drug carrier system

Abstract

A series of poly(acrylic acid)/poly(methyl vinyl ketone) (PAA/PMVK) blends with different compositions were prepared by the solvent casting method. The miscibility of this pair of polymers was investigated by differential scanning calorimetry(DSC), Fourier transform infra-red (FTIR) and X-Ray diffraction (XRD) techniques. An in-vitro cytotoxicity test of the drug-carrier system via MTT (3-(4,5-demethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed no significant cytotoxic effects at concentrations up to 100 µg· ml−1. The STX/PAA-50 drug carrier systems were also prepared by solvent casting of solutions containing the sulfamethoxazole (STX) used as drug model and PAA/PMVK blend in N.N-dimethylformamide then crosslinked with acidified ethylene glycol. The release dynamic of STX from the prepared hydrogels was investigated in which the diffusion through the polymer matrix, the enhancement of the water solubility of STX, the influence of the initial drug concentration, the pH of the medium, and the effect of the degree of swelling of the polymer matrix on the release dynamic was evaluated. According to the total gastrointestinal transit time estimated by Belzer, the estimate distribution of STX released in the different organs indicated that the performance is obtained with the drug – carrier-system containing equal ratios of polymer and 10 wt% of STX (STX-10/PAA-50).

Synthesis and assessment of poly(acrylic acid)/polyvinylpyrrolidone interpenetrating network as a matrix for oral mucosa cells

“…The bands observed in the FTIR spectra of hydrogels (Figure 2) coincided with FTIR spectra reported in literature. 18,19,21,22 Changes in chemical groups between the monomers and polymers were thus demonstrated, which reflects the formation of a new material (i.e. a polymer network).…”

“…These bonds restrict the movement of the polymer, thus increasing the Tg value. 19 The change in Tg between the materials depends on the structure of the polymer. 23–25 Therefore, when there is a change in chemical structure (as in the case of IPN-2%) differences in Tg are observed (Table 1).…”

“…The equilibrium of a polymer network is determined by a balance of osmotic forces originated by the substances that surround it. 19…”

“…However, low biocompatibility of PAAc networks may be a problem for their application. 17–19 We believe that the combination of PAAc and PVP in an IPN allows oral mucosa cells to proliferate.…”

Preparation and Characterization of Poly(vinyl Acetate-co-2-hydroxyethyl Methacrylate) and In Vitro Application as Contact Lens for Acyclovir Delivery

“…Cell adhesion for each sample placed in 24-well plates was assessed using the MTT assay, as described by our previous work [ 62 , 63 ]. Cultured for adhesion overnight, 2 × 10 5 GMSMK cells/specimens were seeded in a 24-well plate.…”