MISFET-based biosensing interface for neurons guided growth and neuronal electrical activities recording

Abstract

International audienceA hybrid circuit of a transistor-based chip was implemented and characterized for the neuronal electrical activity recording. The integration of microfluidic architectures was developed to control precisely neurites outgrowth and form topologically defined and stable neural networks. Individual neural cells from rat retinae and Lymnaea stagnalis snails were immobilized on gates regions of Metal Insulator Semiconductor Field Effect Transistors (MISFET). Neuronal orientation was achieved in both cases but neuronal action potentials were only recorded in the Lymnaea stagnalis case. They were successfully triggered and inhibited by implementing a picrotoxin-GABA – picrotoxin injection protocol, exhibiting a direct influence of picrotoxin on the "spike type" action potential waveform. The implementation of the whole process of neuronal culture and subsequent activity monitoring constitutes a proof-of-principle experiment for the development of neuroelectronic systems for signal processing studies adapted to low-density neuronal cultures

3D arrays of microcages by two-photon lithography for spatial organization of living cells

“…following an established four-step protocol [51][52] Figure 2c. The frame defines a culture chamber with a surface area of 1 cm 2 .…”

Development of All-Around SiO2/Al2O3 Gate, Suspended Silicon Nanowire Chemical Field Effect Transistors Si-nw-ChemFET

“…Once the gate/source/drain metallic contacts and interconnections were ended, Si-nw-MOSFET and Si-nw-ChemFET were fabricated. Then, using a specific SU8-3D technique [7], covered 5 µm width microfluidic channels were finally realized in order to deal with liquid phase analysis at the microscale ( Figure 2). …”

Design, realization and characterization of all-arround SiO2/Al2O3 gate, suspended silicon nanowire chemical field effect transistors

“…Thus, a SiO2/Al2O3 gate was integrated all-around the 500 nm Once the gate/source/drain metallic contacts and interconnections were ended, Si-nw-MOSFET and Si-nw-ChemFET were fabricated (figure 3). Then, using a specific SU8-3D technique [13], covered 5µm width microfluidic channels were finally realized in order to deal with liquid phase analysis at the microscale (figure 4).…”