Misregulation of the Dependence Receptor DCC and its Upstream lincRNA, LOC100287225, in Colorectal Cancer

Kazemzadeh, Mina; Safaralizadeh, Reza; Feizi, Mohammad Ali Hosseinpour; Somi, Mohammad Hossein; Shokoohi, Behrooz

doi:10.5301/tj.5000426

Cited by 11 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GO and KEGG databases were used to conduct GO functional analysis and KEGG signaling pathway analysis on the seven genetic markers so as to establish a primary apoptotic regulatory network, which showed that DCC was involved in the apoptotic signaling pathway with positive regulation. And over these years DCC was reported as one of the key tumor suppressor genes (Kazemzadeh et al, 2015) and was closely correlated to growth and development of colorectal cancer. And it was found that DCC gene can inhibit cell proliferation and cause degraded carcinoembryonic antigen (CEA) expression in rectal cancer cell line SW1116 (Jiang et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Establishment of apoptotic regulatory network for genetic markers of colorectal cancer

Hao

Shan

Kang

2017

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Our purpose is to screen out genetic markers applicable to early diagnosis for colorectal cancer and to establish apoptotic regulatory network model for colorectal cancer, thereby providing theoretical evidence and targeted therapy for early diagnosis of colorectal cancer. Taking databases including CNKI, VIP, Wanfang data, Pub Med, and MEDLINE as main sources of literature retrieval, literatures associated with genetic markers applied to early diagnosis of colorectal cancer were searched to perform comprehensive and quantitative analysis by Meta analysis, hence screening genetic markers used in early diagnosis of colorectal cancer. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were employed to establish apoptotic regulatory network model based on screened genetic markers, and then verification experiment was conducted. Through Meta analysis, seven genetic markers were screened out, including WWOX, K-ras, COX-2, p53, APC, DCC and PTEN, among which DCC shows highest diagnostic efficiency. GO analysis of genetic markers found that six genetic markers played role in biological process, molecular function and cellular component. It was indicated in apoptotic regulatory network built by KEGG analysis and verification experiment that WWOX could promote tumor cell apoptotic in colorectal cancer and elevate expression level of p53. The apoptotic regulatory model of colorectal cancer established in this study provides clinically theoretical evidence and targeted therapy for early diagnosis of colorectal cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Establishment of apoptotic regulatory network for genetic markers of colorectal cancer

Hao

Shan

Kang

2017

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…In mammals, lincRNAs are associated with various cancers, including prostate cancer 16 , breast cancer 18 , colorectal cancer 20 , 21 , ovarian cancer 22 , and bladder cancer 24 , and also associated with disease progression, for example, the HOTAIR (HOX transcript antisense RNA) is highly expressed in the breast cancer and is a predictor for metastasis formation 39 , indicating that lincRNAs could be applied to the prediction and treatment of cancers. However, chicken lincRNAs were only reported to participate in MDV tumorigenesis 36 .…”

Section: Discussionmentioning

confidence: 99%

Long intergenic non-coding RNA GALMD3 in chicken Marek’s disease

Han

Zhang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Long intergenic non-coding RNAs (lincRNAs) are transcribed from non-coding DNA sequences. Studies have revealed that aberrant expressions of lincRNAs are associated with various types of cancers and neurological disorders. Marek’s disease (MD) is a highly contagious T-cell lymphoid neoplasia of chicken induced by Marek’s disease virus (MDV). In this study, we first identified and validated linc-GALMD3 highly expressed in MDV-infected CD4+ T cells by RNA-Seq and qRT-PCR. By RNA-Seq analysis in MDCC-MSB1 cells after loss of function of linc-GALMD3 by shRNA, we found that linc-GALMD3 could positively cis-regulate its downstream gga-miR-223 gene expression. In contrast, it could trans-regulate the 748 differentially expressed genes (FDR < 0.01) that were mainly enriched into mitochondrial structure and cell cycle processes using GO analysis. Of these, the most significantly expressed gene EPYC might cause iris lesion in MD. The other eight genes, NDUFA4, NDUFB6, NDUFV1, NDUFS8, SDHB, UQCRC1, UQCRC2, and COX7A2, actively participated in oxidative phosphorylation in mitochondrial dysfunction and cell death. Most importantly, we found that the MDV replication was repressed when linc-GALMD3 was knocked down in CEF cells. Our results suggested that linc-GALMD3 might be a critical regulator in chicken MD and could be used as a candidate-promising mark for MD prevention, diagnosis, and treatment.

show abstract

“…The results showed that DCC positively regulated the apoptotic signal pathway. It has been found in recent years that DCC is the largest human tumor suppressor gene (Kazemzadeh et al, 2015) closely related to onset and development of colorectal cancer. PTEN is an important tumor suppressor gene with phosphatase activity.…”

Section: Discussionmentioning

confidence: 99%

Establishment of optimal regulatory network of colorectal cancer based on p42.3 protein

Hao¹,

Zhang²,

Shan³

et al. 2017

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

to establish regulatory network of colorectal cancer involving p42.3 protein and to provide theoretical evidence for deep functional exploration of p42.3 protein in the onset and development of colorectal cancer. with protein similarity algorithm, reference protein set of p42.3 cell apoptosis was built according to structural features of p42.3. GO and KEGG databases were used to establish regulatory network of tumor cell apoptosis involving p42.3; meanwhile, the largest possible working pathway that involves p42.3 protein was screened out based on Bayesian network theory. Besides, GO and KEGG were used to build regulatory network on early diagnosis gene markers for colorectal cancer including WWOX, K-ras, COX-2, p53, APC, DCC and PTEN, at the same time, a regulatory network of colorectal cancer cell apoptosis which involves p42.3 was established. cell apoptotic regulatory network that p42.3 participates in primarily consists of Bcl-2 family genes and the largest possible pathway is p42.3 → FKBP → Bcl-2 centered as FKBP protein. Combined with colorectal cancer regulatory network that involves early diagnosis gene markers, it can be predicted that p42.3 is most likely to regulate the colorectal cancer cell apoptosis through FKBP → Bcl-2 → Bax → caspase-9 → caspase-3 pathway. the colorectal cancer apoptosis network based on p42.3 established in the study provides theoretical evidence for deep exploration of p42.3 regulatory mechanism and molecular targeting treatment of colorectal cancer.

show abstract

Misregulation of the Dependence Receptor DCC and its Upstream lincRNA, LOC100287225, in Colorectal Cancer

Abstract: Our study demonstrated the concurrent misregulation of DCC and LOC100287225 in colorectal cancer.

Cited by 11 publications

References 25 publications

WITHDRAWN: Establishment of apoptotic regulatory network for genetic markers of colorectal cancer

WITHDRAWN: Establishment of apoptotic regulatory network for genetic markers of colorectal cancer

Long intergenic non-coding RNA GALMD3 in chicken Marek’s disease

Establishment of optimal regulatory network of colorectal cancer based on p42.3 protein

Contact Info

Product

Resources

About