Mitigated membrane fouling of anammox membrane bioreactor by microbiological immobilization

Zhang, Zuotao; Liu, Sitong; Miyoshi, Takayuki; Matsuyama, Hideto; Ni, Jinren

doi:10.1016/j.biortech.2015.11.037

Cited by 40 publications

(11 citation statements)

References 32 publications

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“…The whole device was wrapped with black cloth to occlude light, which can have a negative impact on bacteria [23,24]. The reactor was filled with the seed anammox sludge with the 1-3 mm (in length) granular activated carbon carriers, which were cultured in a 50 L reactor for 6 years at Guilin University of Technology, China, and showed high biological activity [25][26][27][28][29]. The working volume was 300 mL, and the experimental wastewater enters the reactor through a peristaltic pump.…”

Section: Methodsmentioning

confidence: 99%

Effects of Ca2+ Concentration on Anaerobic Ammonium Oxidation Reactor Microbial Community Structure

Jin

Zhang

2019

Water

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The anaerobic ammonium oxidation (anammox) reaction removes nitrogen from wastewater, the performance of which is influenced by Ca2+; however, the effect of Ca2+ on microbial community structure is unclear. Therefore, the effects of Ca2+ concentration on the treatment performance of an anammox reactor and microbial community structure of anammox sludge were investigated. Ca2+ concentration minimally influenced the removal efficiency of NO2−–N and NH4+–N, but substantially influenced total N removal. Changing the Ca2+ concentration (between 25 and 125 mg/L) caused the average removal rate of total nitrogen to fluctuate by 3.3 percentage points. There were five major bacterial phyla in the anammox sludge: Proteobacteria, Chloroflexi, Acidobacteria, Planctomycete, and Chlorobi. Microbiological analysis revealed that the genera Acidobacterium, Anaerolinea, and Denitratisoma were positively correlated with Ca2+ concentration, and improved treatment performance of the anammox reactor. Moreover, uncultured Chlorobi bacterium clone RUGL1-218 (GQ421108.1) and uncultured sludge bacterium A21b (KT182572.1) may be key microorganisms for the immobilization of anammox bacteria. These findings offer a theoretical basis for improved wastewater treatment using the anammox process.

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Section: Methodsmentioning

confidence: 99%

Effects of Ca2+ Concentration on Anaerobic Ammonium Oxidation Reactor Microbial Community Structure

Jin

Zhang

2019

Water

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“…The sludge inoculated in the R1 and R4 reactors was taken from the partial nitrification sludge and anammox sludge. In addition, the anammox granular sludge was cultivated by the laboratory, and was dark red [7,[21][22][23]; the inoculation amounts were 1 × 10 −3 and 1.5 × 10 −3 m 3 , respectively. This experiment used simulated wastewater from artificial synthesis, and the main components included NH 4 HCO 3 (180 mg/L), KH 2 PO 4 (50 mg/L), NaHCO 3 (prepared on demand), CaCl 2 •2H 2 O (100 mg/L), MgSO 4 •7H 2 O (100 mg/L), and microbial promoter (0.5 mL/L).…”

Section: Inoculated Sludge and Experimental Wastewatermentioning

confidence: 99%

“…However, for the two-stage anammox process in actual application, there remains many issues that require further study, including the complexity of the actual wastewater component, the inhibition effect of the organic carbon source on anammox bacteria, the uncertain process operation effect, the influence of changes in environmental temperature, and other factors on the bacterial activity, and the process efficiency during field application. + -N and NO 2 − -N were measured according to the standard methods protocol [23]. Total nitrogen (TN) was determined using the persulfate method [24].…”

Section: Hrt Experimental Proceduresmentioning

confidence: 99%

Effect of Hydraulic Retention Time on Carbon Sequestration during the Two-Stage Anammox Process

Liu

Xiang

et al. 2019

Processes

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In a biological treatment process, hydraulic retention time (HRT) has a certain effect on the operation of the reactor. This study investigated the effect of HRT on carbon sequestration in a two-stage anaerobic ammonium oxidation (anammox) process using a partial nitrification reactor and anammox reactor to determine the optimal carbon sequestration operating conditions. Molecular biotechnology was used to analyze the sludge in the reactor in order to explore the denitrification performance and to determine the carbon sequestration pathway of the microorganisms. The results show that the partial nitrification stage had the highest carbon sequestration rate (0.319 mg/mg·N) when the nitrogen loading rate (NLR) was 0.44 kg·N/m3/d. The NLR of the anammox stage was 0.13 kg·N/m3/d. When the HRT was 33.4 h, the carbon sequestration of the anammox reaction was at its highest, reaching 0.183 mg/mg·N. The results of microbial analysis show that the carbon-fixing gene cbbLR1 was present in the sludge samples during the anammox and partial nitrification stages, and that there was a Calvin cycle carbon sequestration pathway during the growth process. However, the existence of a gene for reducing and immobilizing CO2 by the acetyl-CoA pathway was not detected; further research is thus needed.

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“…The growth of anammox bacteria is also affected by the initial inoculum present in the immobilized cells. The inoculation may be pure anammox culture or a mixed biomass . Dong et al .…”

Section: Characteristics Of Synthetic Polymeric Gelsmentioning

confidence: 99%

“…46 The growth of anammox bacteria is also affected by the initial inoculum present in the immobilized cells. The inoculation may be pure anammox culture 35,52,53 or a mixed biomass. 48,54,55 Dong et al 56 proposed an optimum inoculum concentration of 1.3 immobilized balls per mL of immobilized medium resulting in the highest nitrite nitrogen concentration of 143.41 mg L −1 .…”

Section: Diffusivity and Growth Of Microorganismsmentioning

confidence: 99%

Research advances and challenges in anammox immobilization for autotrophic nitrogen removal

Manonmani

Joseph

2018

J of Chemical Tech & Biotech

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Application of the anaerobic ammonium oxidation (anammox) process using suspended or attached growth technology has limitations of slow growth rate such as biomass washout, and large footprint. This has led to the development of smaller, compact immobilized systems. In recent years, wastewater treatment using anammox immobilized cells has received much attention due to its advantages of high biomass content, retention of biomass by prevention of washout, good solid–liquid separation and lower toxicity to microorganisms. This article critically reviews the existing literature on autotrophic nitrogen removal using anammox bacteria in its immobilized form. Different methods of using immobilized anammox bacteria were identified. Characteristics of various polymeric gels such as solubility, biodegradability, stability, diffusivity and growth of biomass in the polymeric gel were studied. However, there is insufficient study of disposal methods and costs involved in anammox immobilization to guide future implementation of immobilized anammox technologies for autotrophic nitrogen removal. © 2018 Society of Chemical Industry

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Mitigated membrane fouling of anammox membrane bioreactor by microbiological immobilization

Cited by 40 publications

References 32 publications

Effects of Ca2+ Concentration on Anaerobic Ammonium Oxidation Reactor Microbial Community Structure

Effects of Ca2+ Concentration on Anaerobic Ammonium Oxidation Reactor Microbial Community Structure

Effect of Hydraulic Retention Time on Carbon Sequestration during the Two-Stage Anammox Process

Research advances and challenges in anammox immobilization for autotrophic nitrogen removal

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