Mitigation of the Biofilm Using Nanoparticles By Enhanced by D-alanine and D-proline

Abstract

The present study was undertaken to investigate the susceptibility patterns as antifungal and growth of certain D-amino acids inhibitory effects, includes D-alanine(D-ala) and D-proline (D-pro). The obtained results indicated that D-proline acid is potent mostly as antifungal, among tested D-amino acids, then followed by D-ala. The study aim was evaluating the of D-amino acids and nanoparticles against Candida albicans adhered cells and bio-films. Results showed that D-ala MIC value was 25 μg/ml, while in methods of TCP, (D-pro) MIC 50 μg/ml value. Nanoparticles (lithium and silver) and amino acids (D-pro and D-ala) effect in preventing cells adhesion on polystyrene surface and mature bio-film inhibition was studied. Highest inhibition was obtained at D-pro concentration 50 μg /ml, while the lowest at concentration D-ala 25 μg /ml against mature bio-film and cell adhesion.