Mitochondria in the Nuclei of Rat Myocardial Cells

Eldarov, Chupalav; Vangely, Irina M.; Vays, V. B.; Sheval, Eugene V.; Holtze, Susanne; Hildebrandt, Thomas B.; Колосова, Н.Г.; Popkov, Vasily A.; Plotnikov, Egor Y.; Zorov, Dmitry B.; Bakeeva, L. E.; Skulachev, Vladimir P.

doi:10.3390/cells9030712

Cited by 11 publications

(4 citation statements)

References 58 publications

(83 reference statements)

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“…Intranuclear mitochondria have occasionally been reported in several cell types, including cardiac myocytes [25,26], but the incidence and details regarding the specific mechanism whereby mitochondria appear in the nuclear matrix are unknown. In this study, we did not find any mitochondria inside the nucleus; only we found this organelle in the perinuclear area.…”

Section: Discussionmentioning

confidence: 99%

Electron Microscopy Reveals Evidence of Perinuclear Clustering of Mitochondria in Cardiac Biopsy-Proven Allograft Rejection

Tarazón

Pérez-Carrillo

Portolés

et al. 2022

JPM

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Acute cellular rejection is a major complication in heart transplantation. We focus on the analysis of new ultrastructural findings in cardiac biopsy rejection based on mitochondrial intracellular organization. This study includes heart transplanted patients from a single center who were referred for endomyocardial biopsies as a scheduled routine screening. Participants were divided into two groups: patients transplanted without allograft rejection (Grade 0R), and patients with biopsy-proven allograft rejection (Grade ≥ 2R). Using electronic microscopy, we detected a significant increase in the volume density of mitochondria (p < 0.0001) and dense bodies (p < 0.01) in the rejection group. The most relevant finding was the presence of local accumulations of mitochondria close to the nuclear envelope, pressing and molding the morphology of this membrane in all rejection samples (100%). We identified this perinuclear clustering of mitochondria phenomenon in a 68 ± 27% of the total cardiac nucleus observed from rejection samples. We did not observe this phenomenon in any non-rejection samples, reflecting excellent sensitivity and specificity. We have identified a specific phenomenon affecting the architecture of the nuclear membrane—perinuclear clustering of mitochondria—in endomyocardial biopsies from patients with cardiac rejection. This ultrastructural approach might complement and improve the diagnosis of rejection.

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Section: Discussionmentioning

confidence: 99%

Electron Microscopy Reveals Evidence of Perinuclear Clustering of Mitochondria in Cardiac Biopsy-Proven Allograft Rejection

Tarazón

Pérez-Carrillo

Portolés

et al. 2022

JPM

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“…The incorporation of organelle such as mitochondria has also been observed after NE repair, questioning the fate and consequence of such an intruder on nuclear dynamics [ 69 ]. Interestingly, 1% of rat cardiomyocytes, a cell type that endures persistent mechanostress, present mitochondria in their nucleus, giving more importance to this phenomenon [ 121 ].…”

Section: Chromosomal Instability Associated To Nedmentioning

confidence: 99%

Nuclear Envelope Integrity in Health and Disease: Consequences on Genome Instability and Inflammation

Gauthier

Comaills

2021

IJMS

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The dynamic nature of the nuclear envelope (NE) is often underestimated. The NE protects, regulates, and organizes the eukaryote genome and adapts to epigenetic changes and to its environment. The NE morphology is characterized by a wide range of diversity and abnormality such as invagination and blebbing, and it is a diagnostic factor for pathologies such as cancer. Recently, the micronuclei, a small nucleus that contains a full chromosome or a fragment thereof, has gained much attention. The NE of micronuclei is prone to collapse, leading to DNA release into the cytoplasm with consequences ranging from the activation of the cGAS/STING pathway, an innate immune response, to the creation of chromosomal instability. The discovery of those mechanisms has revolutionized the understanding of some inflammation-related diseases and the origin of complex chromosomal rearrangements, as observed during the initiation of tumorigenesis. Herein, we will highlight the complexity of the NE biology and discuss the clinical symptoms observed in NE-related diseases. The interplay between innate immunity, genomic instability, and nuclear envelope leakage could be a major focus in future years to explain a wide range of diseases and could lead to new classes of therapeutics.

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“…The anatomical and functional connection between mitochondria and the nucleus provides a coordinated cellular response to intracellular changes. The study by Eldarov et al [12] explored the idea that mitochondria interaction with the nucleus is beyond intramembrane connection; it espouses the notion that a tiny fraction of the organelle could reside in the nucleus. This provocative concept has its genesis from earlier studies, but the results at the time generated skepticisms.…”

Section: Mitochondria In Healthmentioning

confidence: 99%

Mitochondria in Health and Diseases

Javadov

Kozlov

Camara

2020

Cells

144

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Mitochondria are subcellular organelles evolved by endosymbiosis of bacteria with eukaryotic cells characteristics. They are the main source of ATP in the cell and play a pivotal role in cell life and cell death. Mitochondria are engaged in the pathogenesis of human diseases and aging directly or indirectly through a broad range of signaling pathways. However, despite an increased interest in mitochondria over the past decades, the mechanisms of mitochondria-mediated cell/organ dysfunction in response to pathological stimuli remain unknown. The Special Issue, “Mitochondria in Health and Diseases,” organized by Cells includes 24 review and original articles that highlight the latest achievements in elucidating the role of mitochondria under physiological (healthy) conditions and, in various cell/animal models of human diseases and, in patients. Altogether, the Special Issue summarizes and discusses different aspects of mitochondrial metabolism and function that open new avenues in understanding mitochondrial biology.

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Mitochondria in the Nuclei of Rat Myocardial Cells

Cited by 11 publications

References 58 publications

Electron Microscopy Reveals Evidence of Perinuclear Clustering of Mitochondria in Cardiac Biopsy-Proven Allograft Rejection

Electron Microscopy Reveals Evidence of Perinuclear Clustering of Mitochondria in Cardiac Biopsy-Proven Allograft Rejection

Nuclear Envelope Integrity in Health and Disease: Consequences on Genome Instability and Inflammation

Mitochondria in Health and Diseases

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