Mitochondria of the Ehrlich ascites-tumour cell. Isolation and studies of oxidative phosphorylation

Diphosphopyridine Nucleotide Specific Isocitric Dehydrogenase of Mammalian Mitochondria. I. On the Roles of Pyridine Nucleotide Transhydrogenase and the Isocitric Dehydrogenases in the Respiration of Mitochondria of Normal and Neoplastic Tissues*

“…Some Properties of the Ascites Mitochondria Preparations. Table I presents data on several oxidative reactions catalyzed by mitochondria of the Ehrlich ascites carcinoma prepared by the method of Hawtrey and Silk (1960). The cytochrome C content appears to be in the normal range; nevertheless, definite stimulation of DPNH and TPNH oxidase activities are obtained by the addition of cytochrome C. The cytochrome C augmented activity shows a sensitivity to amytal and antimycin A similar to that of the endogenous rate.…”

“…Mitochondrial Preparations. Ascites carcinoma mitochondria were prepared essentially as described by Hawtrey and Silk (1960). Ascitic fluid was obtained from 20 to 40 mice inoculated intraperitoneally 6 days previously with suspensions of Ehrlich (Lettre') ascites cells.…”

“…The present studies were prompted in part by the failure of Hawtrey and Silk (1961) to detect pyridine nucleotide transhydrogenase activity in their preparations (Hawtrey and Silk, 1960) of mitochondria of the Ehrlich ascites carcinoma and their finding in this system of significant DICDH activity dependent on unusually high DPN+ concentration. It pyridine nucleotide analog method (Stein et al, 1959) does demonstrate the presence of unsymmetrical pyridine nucleotide transhydrogenase in the same order of magnitude of the activity of DICDH and TICDH measured by following spectrophotometrically the reduction of DPN+ and TPN+ with the pyridine nucleotide depleted mitochondrial preparations.…”

Comparative characteristics of respiration and oxidative phosphorylation in mitochondria of cells of mouse liver and lymphoma NK/Ly

“…Полярографічні криві аналізували за допомогою авторської програми на базі ма тематичного процесора MATLAB [14]. Математично-статистичне опрацювання даних здійснювали з використанням пакету програм Microsoft Excel.…”

Effects of Sarcomycin and SB 21 Derivative on the Metabolism of Ehrlich Ascites Cells

“…T h e re a fte r a p a r t o f th e cell su spension was used for resp iratio n m e a su re m e n ts; from th e re m a in d e r the m ithocondrial and n u clear fra c tio n s w ere p re p a re d b y d ifferen tial cen trifu g atio n s [4]. T o estim ate th e cy to ch ro m e-o x y d ase a c tiv ity th e m ic ro sp e c tro p h o to m etric m ethod according to Cooperstein and Lazarov [5] was em ployed.…”