Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T₂‐weighted imaging and dynamic contrast‐enhanced MRI

Abstract

PurposeTo investigate the effect of mitochondria‐targeted antioxidant MitoQ in reducing the severity of renal ischemia‐reperfusion injury (IRI) in rats using T2‐weighted imaging and dynamic contrast‐enhanced MRI (DCE‐MRI).MethodsIschemia‐reperfusion injury was induced by temporarily clamping the left renal artery. Rats were pretreated with MitoQ or saline. The MRI examination was performed before and after IRI (days 2, 5, 7, and 14). The T2‐weighted standardized signal intensity of the outer stripe of the outer medulla (OSOM) was measured. The unilateral renal clearance rate kcl was derived from DCE‐MRI. Histopathology was evaluated after the final MRI examination.ResultsThe standardized signal intensity of the OSOM on IRI kidneys with MitoQ were lower than those with saline on days 5 and 7 (P = 0.004, P < 0.001, respectively). Kcl values of IRI kidneys with MitoQ were higher than those with saline at all time points (P = 0.002, P < 0.001, P = 0.001, P < 0.001). Histopathology showed that renal damage was the most predominant on the OSOM of IRI kidneys with saline, which was less obvious with MitoQ (P < 0.001).ConclusionsThese findings demonstrate that MitoQ can reduce the severity of renal damage in rodent IRI models using T2‐weighted imaging and DCE‐MRI. Magn Reson Med 79:1559–1667, 2018. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Evaluation of renal ischemia‐reperfusion injury by magnetic resonance imaging texture analysis: An experimental study

“…Hueper et al established a mouse model of renal IRI and indicated that increases in the T 2 signal of the kidney were associated with cell edema caused by acute renal injury after renal IRI 9 . Liu et al investigated the effect of mitochondria‐targeted antioxidant MitoQ on renal IRI in a rat model using T 2 WI, showing that the increase in the T 2 signal of the kidney may be related to the tissue edema and increased water content caused by reperfusion 22 . In this study, T 2 WI_S(3,‐3)InvDfMom had the strongest correlation with brush border destruction, tubular epithelial edema, necrosis, and cast, and it gradually increased from 1 to 24 hours after reperfusion.…”

Antioxidant and Oxidative Stress: A Mutual Interplay in Age-Related Diseases

“…MitoQ is a derivative of ubiquinone which linked to TPP moiety by a 10-carbon alkyl chain (Murphy and Smith, 2007 ). Much information indicates that the ubiquinol moiety of MitoQ can react with superoxide and protect against peroxynitrite (Liu et al, 2018b ). Animal studies have revealed that MitoQ protects against oxidative damage such as hypertension (Pak et al, 2018 ) and neurodegenerative disease (Yin et al, 2016 ).…”

Ischemia and Reperfusion Injury in Kidney Transplantation: Relevant Mechanisms in Injury and Repair

“…In the mitochondria ubiqinone is reduced to ubiquinol, a powerful ROS scavenger. Administration of MitoQ in renal I/R models resulted in reduced markers of oxidative stress, reduced renal injury and improved function [28][29][30]. Regarding the mitochondrial targeted proteins, the Szeto-Schiller (SS) proteins are the best known.…”