Mitochondria-Targeted Sensor Array with Aggregation-Induced Emission Luminogens for Identification of Various Cells

Abstract

Accurate discrimination of cancerous cells is a good solution for early diagnosis of tumors. The mitochondrion plays an important role in cells. Herein, the five aggregation-induced emission luminogens (AIEgens) with various double positive charges are synthesized to image mitochondria. Tetraphenylethylene (TPE) molecules are modified by methoxy groups, conjugated donor–acceptor, and different positive charges to achieve multicolor emission. The five AIEgens form the PTx-Sa (positive mitochondria-target molecular sensor array) to perform cross-fluorescence response based on the mitochondria-targeted imaging to achieve the discrimination of various cells. Principal component analysis of the cross-response fluorescence data of PTx-Sa shows that 100% accurate identification of various cells, including cancer cells and normal cells, digestive tract cancer cells, gastric cancer cells, and mixed gastric cancer cells. By support vector machine to show the predictive ability of PTx-Sa to unknown cells by using blind samples. This is the first time to apply mitochondria-targeted sensor array to identification of various cells.

Multiple-Aptamer-Integrated DNA-Origami-Based Chemical Nose Sensors for Accurate Identification of Cancer Cells

“…Chemical nose sensors have been successfully trained to recognize cancer cells. − For example, isogenic normal and cancerogenic cell lines can be differentiated using nanoparticle–polymer complex sensor arrays . However, most of the reported chemical nose sensors for cancer cell identification are generally tedious and time-consuming (Table S8).…”

Cell-Based Chemical Safety Assessment and Therapeutic Discovery Using Array-Based Sensors

“…For example, they are widely applied to sense different chemical species for monitoring environmental conditions [ 57 , 58 , 59 ] and food quality [ 60 ]. Particularly, array-based sensing platforms are well suited to detect early and subtle changes in complex biosystems present in/on mammalian cells and bacteria [ 61 , 62 , 63 , 64 , 65 ]. Array-based cell sensing often employs interactions of sensors with cell surface components (phospholipids, proteins, and carbohydrates, etc.…”

PEG‐Polymer Encapsulated Aggregation‐Induced Emission Nanoparticles for Tumor Theranostics

“…[103][104][105] In addition, AIEgens also have significant advantages in tracer imaging of subcellular organelles, cells, tissues or organisms. [106][107][108][109][110][111][112] In addition to fluorescence performance, AIEgens have strong ROS generation ability and good photothermal conversion efficiency, so they can also be used for disease treatment. [113][114][115][116] AIEgens are not limited to treatment of tumor, but also have great potential in the field of antibacterial therapy.…”