Mitochondria-targeting phosphorescent iridium(<scp>iii</scp>) complexes for living cell imaging

Zhang, Qingqing; Cao, Rui; Fei, Hao; Zhou, Ming

doi:10.1039/c4dt00823e

Cited by 34 publications

(26 citation statements)

References 48 publications

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“…In previous research, we found that some Ir III complexes possess high specificity for mitochondria and superior photostability, and can be used for mitochondrial imaging and tracking 9. 12c, 16 More importantly, Ir III complexes have been developed as two‐photon mitochondrial probes 16d. e In the present work, to obtain specific novel complexes for mitochondrial two‐photon phosphorescent probes, a series of 2‐phenylimidazo‐[4,5‐][1,10]phenanthroline derivatives that exhibited excellent light‐emitting efficiency were synthesized and conjugated to iridium(III).…”

Section: Introductionmentioning

confidence: 99%

Cyclometalated Iridium(III) Complexes as Two‐Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells

Jin

Liu

Chen

et al. 2015

Chemistry A European J

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Five cyclometalated iridium(III) complexes with 2-phenylimidazo[4,5-f][1,10]phenanthroline derivatives (IrL1-IrL5) were synthesized and developed to image and track mitochondria in living cells under two-photon (750 nm) excitation, with two-photon absorption cross-sections of 48.8-65.5 GM at 750 nm. Confocal microscopy and inductive coupled plasma-mass spectrometry (ICP-MS) demonstrated that these complexes selectively accumulate in mitochondria within 5 min, without needing additional reagents for membrane permeabilization, or replacement of the culture medium. In addition, photobleaching experiments and luminescence measurements confirmed the photostability of these complexes under continuous laser irradiation and physiological pH resistance. Moreover, results using 3D multicellular spheroids demonstrate the proficiency of these two-photon luminescent complexes in deep penetration imaging. Two-photon excitation using such novel complexes of iridium(III) for exclusive visualization of mitochondria in living cells may substantially enhance practical applications of bioimaging and tracking.

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Section: Introductionmentioning

confidence: 99%

Cyclometalated Iridium(III) Complexes as Two‐Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells

Jin

Liu

Chen

et al. 2015

Chemistry A European J

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“…All of these dyes can efficiently image mitochondria in living cell. [15][16][17][18][19][20][21][22][23][24][25][26][27] In addition, some cyclometalated iridium(III) complexes exhibit appealing optical properties, such as aggregation-induced emission, 16,21 two-photon phosphorescence, 22,23 and near infrared emission 18,24 . 10 These organic agents are unable to perform long-time observations due to their poor photostability.…”

Section: Introductionmentioning

confidence: 99%

Cyclometalated iridium(iii) complexes with imidazo[4,5-f][1,10]phenanthroline derivatives for mitochondrial imaging in living cells

et al. 2015

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A new series of cyclometalated iridium(III) complexes with imidazo[4,5-f][1,10]phenanthroline derivatives (i.e., MitoIr1-MitoIr7) were synthesized and developed to image mitochondria in living cells. In comparison with commercially available mitochondrial trackers, these complexes exhibit a superior capacity to selectively accumulate in mitochondria with no requirement of any membrane permeabilization or replacement of the culture medium. In addition, the excellent photostability under continuous laser irradiation as well as the stable physiological pH resistance of these complexes were confirmed by photobleaching experiments and luminescence measurements. Importantly, MitoIr7, which exhibited both excellent luminescence and high ability to locate in mitochondria, was developed to track the mitochondrial morphological changes over a long period of time.

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“…The synthetic routes and the structure characterization of Ir-COOH can refer to the former literature. [41] As shown in Figure 2, beside the well-known absorption in UV regions (< 350 nm), Ir-COOH in this work also has moderate absorption in visible light regions from 450 nm to 600 nm and the molar extinction coefficient (e) is 7750 M À1 cm À1 at 532 nm. Under the excitation of 532 nm (Xe lamp) Ir-COOH in this work has nearinfrared emission with maxima at 713 nm and 783 nm.…”

Section: Main Textmentioning

confidence: 50%

“…The chemical structure of Ir–COOH has been shown in Figure . The synthetic routes and the structure characterization of Ir–COOH can refer to the former literature . As shown in Figure , beside the well‐known absorption in UV regions (< 350 nm), Ir–COOH in this work also has moderate absorption in visible light regions from 450 nm to 600 nm and the molar extinction coefficient (ϵ) is 7750 M −1 cm −1 at 532 nm.…”

Section: Main Textmentioning

confidence: 77%

Green to Blue Annihilated Upconversion from a Simple Iridium(III) Sensitizer with Carboxylic Group

et al. 2016

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A novel iridium(III) complex with carboxylic group (Bis[6‐(benzo[b]thiophen‐2‐yl)phenanthridine‐C3,N](4‐methyl‐4′‐carboxypropyl‐2,2′‐bipyridine)iridium(III)chloride, named Ir–COOH in this work) is investigated for the first time as a sensitizer in triplet‐triplet annihilation (TTA) upconversion studies. Without the aid of bulky incorporated light‐harvesting groups, this simple iridium(III) sensitizer has moderate absorbance in visible light regions (ϵ=7750 M−1cm−1 at 532 nm, ϵ is molar extinction coefficient). Under the assistant of 9,10‐diphenylanthracene (DPA) as a triplet acceptor, green to blue upconversion from Ir–COOH/DPA system is obviously visible under the naked eyes and the upconversion efficiency is 9.5 % in deaerated DMF.

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Mitochondria-targeting phosphorescent iridium(iii) complexes for living cell imaging

Cited by 34 publications

References 48 publications

Cyclometalated Iridium(III) Complexes as Two‐Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells

Cyclometalated Iridium(III) Complexes as Two‐Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells

Cyclometalated iridium(iii) complexes with imidazo[4,5-f][1,10]phenanthroline derivatives for mitochondrial imaging in living cells

Green to Blue Annihilated Upconversion from a Simple Iridium(III) Sensitizer with Carboxylic Group

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