Mitochondrial activity as an indicator of fish freshness

Cléach, Jérôme; Pasdois, Philippe; Marchetti, Philippe; Watier, Denis; Duflos, Guillaume; Goffier, Emmanuelle; Lacoste, Anne-Sophie; Slomianny, Christian; Grard, Thierry; Lencel, Philippe

doi:10.1016/j.foodchem.2019.02.076

Cited by 34 publications

(31 citation statements)

References 36 publications

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“…The mitochondrial structure was altered during storage, due to the reduction in respiratory activity and membrane potential disrupt. However until the fourth day of storage, respiratory activity remained significant (Cléach et al, 2019). These results indicate that mitochondria may be applied as a target to evaluate fish freshness (Cléach et al, 2019).…”

Section: State Of the Art Of Instrumental Methods For Fish Quality Momentioning

confidence: 83%

“…Moreover, fish microbiome is influenced by fish trophic level (Liu et al, 2016), which can lead to variations in pH, preventing its use as a standard method. Cléach et al (2019) evaluated the postmortem changes in mitochondrial structure and function to determinate fish freshness. Mitochondria plays a key role in the mechanisms involving cell death, such as necrosis and apoptosis (Parsons & Green, 2010).…”

Section: State Of the Art Of Instrumental Methods For Fish Quality Momentioning

confidence: 99%

“…Mitochondria plays a key role in the mechanisms involving cell death, such as necrosis and apoptosis (Parsons & Green, 2010). The mitochondria of gilthead seabream (Sparus aurata) stored at 4 • C was isolated and analyzed for oxygen consumption and membrane potential (Cléach et al, 2019). The mitochondrial structure was altered during storage, due to the reduction in respiratory activity and membrane potential disrupt.…”

Section: State Of the Art Of Instrumental Methods For Fish Quality Momentioning

confidence: 99%

“…The SBT, although fast and objective, reflects more the state of spoilage than the state of freshness, as they detect chemical and microbiological changes during the late stages of storage (Cléach et al, 2019). Moreover, these novel technologies still have structural (physical) and methodological (chemical) limitations that make their use defiant.…”

Section: Challenges In the Application Of Aqimentioning

confidence: 99%

“…Thus, as well as QIM, the emerging instrumental methods previously described also have their weaknesses. In this role, alternative technologies, such as molecular approaches and mitochondrial activity monitoring have been developed (Cléach et al, 2019;Costa et al, 2019). Li et al (2017) applied QIM associated with proteomic approaches to evaluate the quality of turbot (Scophthalmus maximus).…”

Section: Challenges In the Application Of Aqimentioning

confidence: 99%

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Fish Quality Index Method: Principles, weaknesses, validation, and alternatives—A review

Bernardo

Rosário

Delgado

et al. 2020

Comp Rev Food Sci Food Safe

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Fish is a high nutritional value matrix of which production and consumption have been increasing in the last years. Advancements in the efficient evaluation of freshness are essential to optimize the quality assessment, to improve consumer safety, and to reduce raw material losses. Therefore, it is necessary to use rapid, nondestructive, and objective methodologies to evaluate the quality of this matrix. Quality Index Method (QIM) is a tool applied to indicate fish freshness through a sensory evaluation performed by a group of assessors. However, the use of QIM as an official method for quality assessment is limited by the protocol, sampling size, specificities of the species, storage conditions, and assessor's experience, which make this method subjective. Also, QIM may present divergences regarding the development of microorganisms and chemical analysis. In this way, novel quality evaluation methods such as electronic noses, electronic tongues, machine vision system, and colorimetric sensors have been proposed, and novel technologies such as proteomics and mitochondrial analysis have been developed. In this review, the weaknesses of QIM were exposed, and novel methodologies for quality evaluation were presented. The consolidation of these novel methodologies and their use as methods of quality assessment are an alternative to sensory methods, and their understanding enables a more effective fish quality control.

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Section: State Of the Art Of Instrumental Methods For Fish Quality Momentioning

confidence: 83%

Section: State Of the Art Of Instrumental Methods For Fish Quality Momentioning

confidence: 99%

Section: State Of the Art Of Instrumental Methods For Fish Quality Momentioning

confidence: 99%

Section: Challenges In the Application Of Aqimentioning

confidence: 99%

Section: Challenges In the Application Of Aqimentioning

confidence: 99%

See 3 more Smart Citations

Fish Quality Index Method: Principles, weaknesses, validation, and alternatives—A review

Bernardo

Rosário

Delgado

et al. 2020

Comp Rev Food Sci Food Safe

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Research on apoptotic mechanism and related pathways involved in postmortem grass carp (Ctenopharyngodon idellus) muscle

Tie

Jiang

et al. 2022

J Sci Food Agric

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BACKGROUND Apoptosis activation is an essential research to reveal the triggering mechanism of flesh quality deterioration. This study was aimed at explaining apoptotic mechanism of postmortem fish in terms of caspases activation, cytochrome c (cyt‐c) release, B‐cell lymphoma 2 (Bcl‐2) and Bcl2‐associated X (Bax) protein levels, transcriptional levels of its molecules, and apoptosis‐inducing factor (AIF) translocation at 4 °C for 5 days. RESULTS Activation of caspase‐9, caspase‐8, caspase‐3 and the release of mitochondrial cyt‐c were observed during storage. The decreased Bcl‐2 protein levels, increased Bax protein expressions and Bax/Bcl‐2 ratio were major steps for inducing apoptosis. Collectively, transcriptional regulation of Fas ligand (FasL), apoptotic protease activating factor‐1 (Apaf‐1), inhibitors of apoptosis proteins (IAPs), myeloid cell leukemia‐1 (Mcl‐1), c‐Jun N‐terminal kinase (JNK) and p38 mitogen‐activated protein kinase (MAPK) indicated that extrinsic apoptotic pathways (FasL/caspase‐8/caspase‐3) and intrinsic pathway [(JNK and p38 MAPK)/(Bcl‐2, Bax and Mcl‐1)/cyt‐c/Apaf‐1/caspase‐9/caspase‐3] were involved in apoptotic process. Mitochondrial AIF translocation to nuclear indicated that AIF mediated caspase‐independent pathway. CONCLUSION Therefore, transcriptional and translational alterations of multiple signaling molecules acted important roles in regulating apoptosis activation in postmortem process. © 2022 Society of Chemical Industry.

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Stunning methods before slaughter induce oxidation changes of large yellow croaker during cold storage: the role of mitochondria and underlying mechanisms

et al. 2023

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BACKGROUNDThe improper stunning methods before slaughter could cause the fish to deteriorate more quickly during cold storage. However, it is unclear that how stunning methods affect the mitochondria structure and the role of mitochondria in the oxidation in muscle‐based food.RESULTSThis study was to explore the potential mechanism of oxidation induced by different stunning methods (hit on the head, T1; gill cut, T2; immersion in ice/water slurry, T3; CO2 asphyxiated, T4; 40% CO2+30% N2+30% O2, T5) in large yellow croaker during cold storage. The results showed that T4 samples had the minimum stress response and the mitochondrial membrane potential and permeability were less damaged. Besides, the mitochondrial functional structure and peroxisome of T4 samples were less damaged compared with other samples, which was reflected in higher total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities. In terms of oxidation indices, the T4 samples showed higher pH values and iron myoglobin (MMb) contents, lower total volatile basic nitrogen (TVB‐N), thiobarbituric acid reactive substances (TBARS) after 168 h cold storage, indicating that the T4 samples significantly maintained the oxidative stability of the large yellow croaker.CONCLUSIONThe CO2 asphyxiation had the least oxidative damage to large yellow croaker during cold storage, possibly because it had the least effect on mitochondrial structure, reactive oxygen species (ROS), and antioxidant enzyme activity.This article is protected by copyright. All rights reserved.

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Mitochondrial activity as an indicator of fish freshness

Cited by 34 publications

References 36 publications

Fish Quality Index Method: Principles, weaknesses, validation, and alternatives—A review

Fish Quality Index Method: Principles, weaknesses, validation, and alternatives—A review

Research on apoptotic mechanism and related pathways involved in postmortem grass carp (Ctenopharyngodon idellus) muscle

Stunning methods before slaughter induce oxidation changes of large yellow croaker during cold storage: the role of mitochondria and underlying mechanisms

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