Mitochondrial DNA from the scallop Pecten maximus: an unusual polymorphism detected by restriction fragment length polymorphism analysis

Abstract

We present an analysis of mitochondrial DNA diversity within the scallop Pecten inaximus. Twenty-seven individuals of Pecten maximus were sampled from three populations from the west coast of Brittany (France) and their mtDNA assayed for restriction site polymorphism. Thirty-four variable and conserved cleavage sites have been mapped. They occur in the coding region as well as in the noncoding one, which has also previously been shown to be variable in length. Nucleotide divergence between two P maximus mtDNA types can reach 3.4 per cent and the nucleotide diversity is 1 per cent. Data using seven restriction endonucleases clearly distinguished 27 genotypes; each individual possessed a unique mitochondrial genotype. Phylogenies derived from mtDNA restriction analysis are not concordant with data on reproductive behaviour or ecological studies. The three populations sampled present similar distributions of the different mitochondrial genotypes, although the scallop stock of the Bay of Saint Brieuc is considered, from physiological parameters, to be genetically distinct from all other populations. Therefore, we suggest that P maximus should fall into category IV of phylogeographical pattern, i.e. continuous mtDNA genotypes with a high gene flow.

Population genetic subdivision in the New Zealand greenshell mussel (Perna canaliculus) inferred from single‐strand conformation polymorphism analysis of mitochondrial DNA

“…1986), undetected genetic variation in allozymes (Nei 1987), balancing selection acting on allozymes (Karl & Avise 1992; but see McDonald et al . 1996), neutrality of allozymes and directional selection acting on mtDNA (Hare & Avise 1998), and historical factors as a consequence of differential evolutionary forces acting on mitochondrial and nuclear genomes (Rigaa et al . 1997).…”

Multiplex PCR sets of novel microsatellite loci for the great scallopPecten maximusand their application in parentage assignment

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Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Sensitive Identification of Ostrich Meat

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