Mitochondrial DNA integrity and copy number in sperm from infertile men

Song, Gyun Jee; Lewis, Vivian

doi:10.1016/j.fertnstert.2007.10.059

Cited by 113 publications

(102 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies have shown that a deficiency in semen antioxidants is associated with sperm DNA damage, whereas, other studies have not observed such a relationship [6,[36][37][38][39]. Similarly, some studies have found that seminal antioxidant activity is reduced in infertile men with high levels of seminal ROS (relative to those with normal levels of ROS) whereas others have not shown this [14,[40][41][42].…”

Section: Semen Antioxidants and Sperm Dna Damagementioning

confidence: 99%

Antioxidants and sperm DNA damage: a clinical perspective

Zini

Gabriel

Baazeem

2009

J Assist Reprod Genet

178

110

View full text Add to dashboard Cite

Purpose Infertile men possess substantially more sperm DNA damage than do fertile men, damage that may impact negatively on reproductive outcomes. In this era of assisted reproductive technologies there is mounting concern regarding the safety of utilizing DNA-damaged spermatozoa in this setting. Therefore, it is important to identify strategies that may reduce sperm DNA damage. The purpose of this review is to discuss the rationale for antioxidant therapy in men with sperm DNA damage and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm DNA damage. Methods We reviewed the literature on antioxidants and sperm DNA damage. Results To date, the data suggest that dietary antioxidants may be beneficial in reducing sperm DNA damage, particularly, in men with high levels of DNA fragmentation. However, the mechanism of action of dietary antioxidants has not been established and most of the clinical studies are small. A beneficial effect of in vitro antioxidant supplements in protecting sperm DNA from exogenous oxidants has been demonstrated, however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established.

show abstract

Section: Semen Antioxidants and Sperm Dna Damagementioning

confidence: 99%

Antioxidants and sperm DNA damage: a clinical perspective

Zini

Gabriel

Baazeem

2009

J Assist Reprod Genet

178

110

View full text Add to dashboard Cite

show abstract

“…We used quantitative real-time PCR (qPCR) to determine the amount of mtDNA relative to nDNA by measuring the ratio of the encoding mitochondrial gene 16S rRNA, and a nuclear gene, GAPDH [8]. Total DNA was extracted from each semen sample by using a DNA isolation kit (Sangon, China) according to the manufacturer's instructions.…”

Section: Analysis Of Sperm Mtdna Copy Numbermentioning

confidence: 99%

“…It has been well documented that the integrity of sperm mtDNA is disrupted in many infertile men [8,9]. To investigate whether the disruption also exists in sperm mtDNA from those living at high altitude, we examined the integrity of a specific mtDNA fragment (8.7 kb) using PCR amplification.…”

Section: Integrity Evaluation Of Sperm Mtdnamentioning

confidence: 99%

“…We applied long PCR amplification to 8.7 kb out of the 16-kb mitochondrial genome in all samples. This 8.7-kb fragment contains several genes encoding subunits of respiratory complexes as well as the common deletion types (4.9 kb and 7.4 kb) [8]. Half of the mitochondrial genome (8.7 kb) was amplified with high fidelity long-Taq polymerase (Takara, Japan).…”

Section: Integrity Evaluation Of Sperm Mtdnamentioning

confidence: 99%

“…The copy number of mtDNA (i.e., the number of mtDNA per spermatozoon) is one of the major mitochondrial genetic features, and plays a critical role in mtDNA-mediated pathological pathways [4][5][6][7]. For example, in infertile men with abnormal sperm quality, mtDNA content was found to be significantly increased, and could serve as a marker for spermatogenic dysfunction [8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Altitude can alter the mtDNA copy number and nDNA integrity in sperm

Luo

Liao

Chen

et al. 2011

J Assist Reprod Genet

View full text Add to dashboard Cite

Objective Mitochondria are factories for energy production and genetic alterations in mtDNA will directly impact OXPHOS function. The copy number of mtDNA (i.e., the number of mtDNA per spermatozoon) is one of the major mitochondrial genetic features. Besides mtDNA copy number, the change of either mtDNA or nDNA integrity is another important factor causing asthenospermia, or poor sperm motility in infertile men. In this study, we investigated the mtDNA copy number and the integrities of mtDNA and nDNA respectively in semen samples from different donors at 5,300 m altitudes. Methods Total DNA was extracted from semen samples from donors in two different altitudes. Quantitative PCR was performed to evaluate the mtDNA copy number. PCR amplification was used to examine the integrity of sperm mtDNA. Flow cytometry was carried out to investigate sperm nDNA integrity. All data were analyzed to show the statistical significance. Results Sperm mtDNA copy number for those living at high altitude (5,300 m) for one month was significantly higher (P<0.05) than for those at the lower altitude (1,400 m) or in donors who had been living at the 5,300 m altitude for 1 year. In addition, sperm mtDNA copy numbers were remarkably decreased (P<0.05) in those who had lived at the greater altitude for 1 year compared to those who had lived there for one month. The ratio of nDNA integrity among the 10,000 sperms at high altitude for one month was significantly lower (P<0.05) than that at the lower altitude (1,400 m) or at 5,300 m for 1 year, and the ratio of nDNA integrity sperms at high altitude for 1 year was increased, and higher than that for at the lower altitude (P<0.05). Conclusion High altitude can alter the mtDNA copy number and nDNA integrity in the sperms.

show abstract

Are old males still good males and can females tell the difference?

Johnson

Gemmell

2012

BioEssays

View full text Add to dashboard Cite

Sperm function generally declines with male age. Paradoxically, females of many species still choose to mate with old males rather than young males. Females choosing old mates may suffer reduced fertilization rates and an increased incidence of birth defects in offspring, lowering fitness which may in turn lead to conflict between the sexes. This apparent paradox has generated much interest from theorists, but whether this paradox presents in nature remains equivocal. Empirical studies have found mixed support for both a decline in fertility with male age and age-based female mate preference. Here, we examine recent evidence for this paradox, identify confounding variables, highlight areas that deserve further investigation, and suggest avenues for future research.

show abstract

Mitochondrial DNA integrity and copy number in sperm from infertile men

Cited by 113 publications

References 33 publications

Antioxidants and sperm DNA damage: a clinical perspective

Antioxidants and sperm DNA damage: a clinical perspective

Altitude can alter the mtDNA copy number and nDNA integrity in sperm

Are old males still good males and can females tell the difference?

Contact Info

Product

Resources

About