Mitochondrial DNAs of Suillus: three fold size change in molecules that share a common gene order

Abstract

We constructed restriction-site and gene maps for mitochondrial DNAs from seven isolates of five species of Suillus (Boletaceae, Basidiomycotina). Each mitochondrial genome exists as a single circular chromosome, ranging in size from 36 to 121 kb. Comparisons within species and between two closely related species revealed that insertions and deletions are the major form of genome change, whereas most restriction sites are conserved. Among more distantly related species, size and restriction-site differences were too great to allow precise alignments of maps, but small clusters of putatively homologous restriction sites were found. Two mitochondrial gene orders exist in the five species. These orders differ only by the relative positions of the genes for ATPase subunit 9 and the small ribosomal RNA and are interconvertible by a single transposition. One of the two gene arrangements is shared by four species whose mitochondrial DNAs span the entire size range of 36 to 121 kb. The conservation of gene order in molecules that vary over three-fold in size and share few restriction sites demonstrates a low frequency of rearrangements relative to insertions, deletions, and base substitutions.

Use of atp6 in Fungal Phylogenetics: An Example from the Boletales

“…We chose atp6 and cox3 because we did not expect large introns to be common in those genes. This expectation was based on known atp6 and cox3 sequences, which were primarily from ascomycetes, and on an early mitochondrial mapping study within the Boletales (Bruns et al, 1988;. We also used atp6 to examine phylogenetic relationships in the Boletales.…”

Analysis of mitochondrial DNA restriction fragment length polymorphisms for examining genetic variability among isolates of Phellinus linteus

“…Kalchbr. (Bruns et al 1988) to 178 kb for A. bitorquis (Hintz et al 1985), and similar to those for L. edodes (Fukuda et al 1994) and Pleurotus ostreatus (Matsumoto and Fukumasa-Nakai 1995).…”

Genetic variation in cultivated strains of Agaricus blazei

“…Kalchbr. (Bruns et al 1988) to A. bitorquis (Hintz et al 1985), and similar to 98.3 kb for A. bisporus (Hintz et al 1985). In addition, mtDNA RFLP patterns have been used as markers to monitor cytoplasmic inheritance in matings between sexually compatible strains, as studied in A. bitorquis (Hintz et al 1988), L. edodes (Fukuda et al 1995), and P. ostreatus (Matsumoto and Fukumasa-Nakai 1996).…”