Mitochondrial genome diversity in connection with male sterility in Allium schoenoprasum L.

Engelke, Thomas; Tatlioglu, T.

doi:10.1007/s001220051374

Cited by 22 publications

(17 citation statements)

References 29 publications

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“…Based on the results of RFLP analyses, we previously discussed a possible function for the atp 9 gene in the generation of the different cytoplasm types in chives; a heterologous probe containing an atp 9 gene from Arabidopsis thaliana mtDNA (GenBank Accession No. NC001284;Unseld et al 1997) detected more fragments than did any of the other mitochondrial gene probes used (Engelke and Tatlioglu 2000). This result suggested that at least parts of the coding or flanking regions of atp 9 are repeated in the mitochondrial genome of chives.…”

Section: Introductionmentioning

confidence: 72%

“…atp 9-RACEse and SplinkerHindIIIse are sense primers; atp 9-RACEas and CMS 1 -RACEas are antisense primers. Amplification with each primer combination was tested in a temperature gradient PCR, including eight different annealing temperatures (from the left): 44.5, 45.9, 48.2, 50.7, 53.2, 55.8, 58.1 and 59.5°C The arrows indicate the amplification products: 848 bp = atp 9-1; 1466 bp = CMS 1 -1; 459 bp = atp 9-2; 1087 bp = CMS 1 -2 of the same species, it is remarkable that in many cases coding or flanking regions of the atp 9 gene are involved in the recombination events that give rise to the chimeric structures, as was briefly reviewed in an earlier paper (Engelke and Tatlioglu 2000). Besides the examples given therein, changes in the transcription of the atp 9 gene seem to be associated with the male sterile cytoplasm PEF1 in sunflower, since nuclear genes that restore fertility specifically rectified alterations in atp 9 transcript patterns in the CMS line (de la Canal et al 2001).…”

Section: Discussionmentioning

confidence: 95%

“…A 5-lg aliquot of total DNA, isolated as previously described by Engelke and Tatlioglu (2000), was digested in a 20-ll reaction volume with 10 U of the appropriate restriction enzyme for 3 h at 37°C using the buffer recommended by the supplier (Fermentas). The reaction was terminated by heat inactivation for 10 min at 65°C.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In chives ( Allium schoenoprasum L.) five types of cytoplasm are known (the sterility-inducing types S1, and S2 and the normal types, N1, N2 and N3), and the mitochondrial genome associated with the sterility-inducing cytoplasm (S 1 ) of the first cytoplasmic male sterility system (CMS 1 ) differs from those of the other four types by numerous RFLPs (Engelke and Tatlioglu 2000). This has greatly complicated the search for the gene responsible for male sterility in this system, relative to other CMS-systems such as the PET1 system in Helianthus annuus (Ko¨hler et al 1991;Laver et al 1991).…”

Section: Introductionmentioning

confidence: 99%

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The fertility restorer genes X and T alter the transcripts of a novel mitochondrial gene implicated in CMS1 in chives ( Allium schoenoprasum L.)

Engelke

Tatlioglu

2004

Mol Genet Genomics

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A chimeric mitochondrial gene configuration, mainly derived from sequences associated with the essential genes atp9 and atp6, was isolated from the sterility-inducing cytoplasm of the CMS1 system in chives (Allium schoenoprasum L.). This sequence is not found in four other cytoplasm types from chives; however, two copies are present in the mitochondrial DNA of CMS1-inducing cytoplasm, whose 5'-sequences are homologous to those of the atp9 gene. We provide evidence to show that one of the two CMS1-specific copies is actively transcribed, and two transcripts which terminate at the same position but differ in their 5'initiation sites were localized using the RACE technique. These transcripts of 942 and 961 nt, respectively, were confirmed to be the major products of this gene in CMS1 plants by Northern hybridization. However, smaller transcripts were found to accumulate in plants in which fertility had been restored. Restoration of fertility was induced either by the gene X, or the gene T at high temperatures. In (S1) X. genotypes a transcript with an estimated size of 440 nt was detected in all tissues examined. An additional hybridization signal with an estimated size of approximately 850 nt is expressed in temperature-sensitive plants [(S1) xxT.], and the intensity of a minor 350-nt transcript is enhanced. These latter alterations, conditioned by the gene T, occur independently of the growth temperature, but are limited to the flowers; they were not observed in leaves. The CMS1 transcripts are edited at seven positions and contain an ORF with a maximum coding capacity of 780 nt (containing the start codon derived from the atp9 gene in-frame). Use of the third in-frame start codon would result in the synthesis of a protein of a size very close to that of a previously described CMS1-specific protein, which has an apparent molecular weight of 18 kDa. The coding sequence that begins at this third in-frame start codon is also present in the sterility-inducing cytoplasms (S) and (T) in the onion, and absent in (N) cytoplasm.

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Section: Introductionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 95%

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The fertility restorer genes X and T alter the transcripts of a novel mitochondrial gene implicated in CMS1 in chives ( Allium schoenoprasum L.)

Engelke

Tatlioglu

2004

Mol Genet Genomics

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“…Isolation of DNA, primer-design and PCR-amplifications Highly purified total DNA was isolated as previously described (Engelke and Tatlioglu 2000b). When the PCR-protocols have been optimised, an alternative DNA-isolation protocol, originally described by Edwards et al (1991), was used, which is much less-time and cost intensive and needs very less plant material.…”

Section: Plant Materialsmentioning

confidence: 99%

A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.)

2003

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The chimerical mitochondrial CMS(1)-specific sequence in chives ( Allium schoenoprasum) was used to develop a PCR-marker that distinguishes both male-sterility inducing cytoplasms, CMS-(S) and CMS-(T), from the normal cytoplasm in onion ( Allium cepa). In combination with a previously described marker for CMS-(S), which anchors in the upstream region of the mitochondrial gene cob, all of the three known cytoplasms in the onion are distinguishable. The PCR-marker system was tested in 361 onion plants, which were selected from F(1)-hybrids and different open-pollinated varieties. The latter are mainly landraces from Turkey, in which all three cytoplasm types were detected.

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Cytoplasmic Male Sterilities and Mitochondrial Gene Mutations in Plants

Budar¹,

Berthomé²

Plant Mitochondria

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Mitochondrial genome diversity in connection with male sterility in Allium schoenoprasum L.

Cited by 22 publications

References 29 publications

The fertility restorer genes X and T alter the transcripts of a novel mitochondrial gene implicated in CMS1 in chives ( Allium schoenoprasum L.)

The fertility restorer genes X and T alter the transcripts of a novel mitochondrial gene implicated in CMS1 in chives ( Allium schoenoprasum L.)

A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.)

Cytoplasmic Male Sterilities and Mitochondrial Gene Mutations in Plants

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