Mitochondrial genome of Scutogyrus longicornis (Monogenea: Dactylogyridea), a parasite of Nile tilapia Oreochromis niloticus

Caña-Bozada, Víctor; Llera-Herrera, Raúl; Fájer-Ávila, Emma Josefina; Morales-Serna, Francisco Neptalí

doi:10.1016/j.parint.2020.102281

Cited by 15 publications

(16 citation statements)

References 53 publications

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“…The mitochondrial genome of C. casuarinus comprises 12 (all except atp 8) intron free protein coding genes, 22 tRNA genes and two genes coding for the large and small subunits of the mitochondrial rRNA following the gene order of other species of Cichlidogyrus (see Table 2 ) [ 76 ]. The absence of atp 8 was detected as in other neodermatan flatworms [ 85 ]. We report an abbreviated stop codon TA for nad 2 as previously observed in C. halli and C. mbirizei [ 76 ].…”

Section: Resultsmentioning

confidence: 99%

“…A high level of genomic diversity in mitochondria including numerous rearrangements has been previously reported in monogeneans [ 76 ], other parasitic or endosymbiotic [ 105 ] and free-living flatworms [ 106 ]. Comparisons at the family level of Dactylogyridae revealed tRNA gene transposition of trn T and between trn L2 and trn R (reviewed in [ 85 ]). Unlike in other genera of parasitic flatworms, such as Schistosoma spp.…”

Section: Discussionmentioning

confidence: 99%

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Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika

Kmentová

Hahn

Koblmüller

et al. 2021

Biology

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Little phylogeographic structure is presumed for highly mobile species in pelagic zones. Lake Tanganyika is a unique ecosystem with a speciose and largely endemic fauna famous for its remarkable evolutionary history. In bathybatine cichlid fishes, the pattern of lake-wide population differentiation differs among species. We assessed the congruence between the phylogeographic structure of bathybatine cichlids and their parasitic flatworm Cichlidogyrus casuarinus to test the magnifying glass hypothesis. Additionally, we evaluated the use of a PoolSeq approach to study intraspecific variation in dactylogyrid monogeneans. The lake-wide population structure of C. casuarinus ex Hemibates stenosoma was assessed based on a portion of the cox1 gene combined with morphological characterisation. Additionally, intraspecific mitogenomic variation among 80 parasite samples from one spatially constrained metapopulation was assessed using shotgun NGS. While no clear geographic genetic structure was detected in parasites, both geographic and host-related phenotypic variation was apparent. The incongruence with the genetic north-south gradient observed in H. stenosoma may be explained by the broad host range of this flatworm including eupelagic bathybatine host species that form panmictic populations across the lake. In addition, we present the first parasite mitogenome from Lake Tanganyika and propose a methodological framework for studying the intraspecific mitogenomic variation of dactylogyrid monogeneans.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika

Kmentová

Hahn

Koblmüller

et al. 2021

Biology

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show abstract

“…However, in the case of nad2, an overlap of 1 bp with trnV would allow the presence of the canonical stop codon TAG as was reported in the annotation of C. sclerosus (JQ038226, unpublished). Truncated stop codons have been reported across different lineages of parasitic [68,96,104], endosymbiotic [94] and free living flatworm taxa [105] , and also in early diverging acoelomorphs [106]. An alternative start codon ATT was previously assembled for several flatworm taxa [94,98,107,108] but here it is reported for the first time in dactylogyrid monogeneans, as the start codon of the nad1 gene in C. casuarinus.…”

Section: Mitochondrial Genome Lake Tanganyika and The Rest Of The Monogenean Worldmentioning

confidence: 87%

“…A high level of genomic diversity in mitochondria including numerous rearrangements has been previously reported in monogeneans [68], other parasitic or endosymbiotic [94] and free living flatworms [95]. Comparisons at the family level of Dactylogyridae revealed tRNA gene transposition of trnT and between trnL2 and trnR (reviewed in [96]). Unlike in other genera of parasitic flatworms, such as Schistosoma spp.…”

Section: Mitochondrial Genome Lake Tanganyika and The Rest Of The Monogenean Worldmentioning

confidence: 87%

Contrasting Host-parasite Population Structure and Role of Host Specificity: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika

Kmentová¹,

Hahn²,

Koblmüller³

et al. 2021

Preprint

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Little phylogeographic structure is presumed for highly mobile species in pelagic zones. Lake Tanganyika is a unique ecosystem with a speciose and largely endemic fauna famous for its remarkable evolutionary history. In bathybatine cichlid fishes, the pattern of lake-wide population differentiation differs among species. We tested the magnifying glass hypothesis for their parasitic flatworm Cichlidogyrus casuarinus. Lake-wide population structure of C. casuarinus ex Hemibates stenosoma was assessed based on a portion of the mtCOI gene combined with morphological characterisation. Additionally, intraspecific mitogenomic variation among 80 individuals within one spatially constrained parasite metapopulation sample was assessed using shotgun NGS. While no clear geographic genetic structure was detected in parasites, both geographic and host-related phenotypic variation was apparent. The incongruence with the genetic north-south gradient observed in the host may be explained by the broad host range of this flatworm as some of its other host species previously showed no lake-wide restriction of gene flow. Our results are consistent with host driven morphological variation without genetic differentiation of the parasite, and highlight the importance of integratively approaching parasites` potential as “tags” for their hosts. We present the first parasite mitogenome from Lake Tanganyika and propose a methodological framework for studying intraspecific mitogenomic variation of dactylogyrid monogeneans.

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“…The cichlid-Cichlidogyrus species network has even been proposed as model system for macroevolutionary research in host-parasite systems Vanhove et al, 2016) because of this diversity. Recent advances in immunological (Zhi et al, 2018), pathological (Igeh and Avenant Oldewage, 2020), genomic (Vanhove et al, 2018;Caña-Bozada et al, 2021), and microscopy research (Fannes et al, 2015) have brought the vision of a cichlid-Cichlidogyrus model system closer to reality (see Cruz-Laufer et al, 2020 for a detailed review of model system qualities).…”

Section: Species-rich But Missing Data: a Case Study For Host-parasite Systemsmentioning

confidence: 99%

Somewhere I belong: phylogeny and morphological evolution in a species-rich lineage of ectoparasitic flatworms infecting cichlid fishes

Cruz-Laufer

Pariselle

Jorissen

et al. 2021

Preprint

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Metazoan parasites encompass a significant portion of the global biodiversity. Their relevance for environmental and human health calls for a better understanding as parasite macroevolution remains mostly understudied. Yet limited molecular, phenotypic, and ecological data have so far discouraged complex analyses of evolutionary mechanisms and encouraged the use of data discretisation and body-size correction. In this case study, we aim to highlight the limitations of these methods and propose new methods optimised for small datasets. We apply multivariate phylogenetic comparative methods (PCMs) and statistical classification using support vector machines (SVMs) to a data-deficient host-parasite system. We use continuous morphometric and host range data currently widely inferred from a species-rich lineage of parasites (Cichlidogyrus incl. Scutogyrus - Platyhelminthes: Monogenea, Dactylogyridae) infecting cichlid fishes. For PCMs, we modelled the attachment organ and host range evolution using the data of 135 species and an updated multi-marker (28S and 18S rDNA, ITS1, COI mtDNA) phylogenetic reconstruction of 58/137 described species. Through a cluster analysis, SVM-based classification, and taxonomic literature survey, we infered the systematic informativeness of discretised and continuous characters. We demonstrate that an update to character coding and size-correction techniques is required as some techniques mask phylogenetic signals but remain useful for characterising species groups of Cichlidogyrus. Regarding the attachment organ evolution, PCMs suggest a pattern associated with genetic drift. Yet host and environmental parameters might put this structure under stabilising selection as indicated by a limited morphological variation. This contradiction, the absence of a phylogenetic signal and multicollinearity in most measurements, a moderate 73% accordance rate of taxonomic approach and SVMs, and a low phylogenetic informativeness of reproductive organ data suggest an overall limited systematic value of the measurements included in most species characterisations. We conclude that PCMs and SVM-based approaches are suitable tools to investigate the character evolution of data-deficient taxa.

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Mitochondrial genome of Scutogyrus longicornis (Monogenea: Dactylogyridea), a parasite of Nile tilapia Oreochromis niloticus

Cited by 15 publications

References 53 publications

Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika

Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika

Contrasting Host-parasite Population Structure and Role of Host Specificity: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika

Somewhere I belong: phylogeny and morphological evolution in a species-rich lineage of ectoparasitic flatworms infecting cichlid fishes

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