Mitochondrial genomes of Sternochetus species (Coleoptera: Curculionidae) and the phylogenetic implications

Abstract

The three weevil species, Sternochetus gravis, S. mangiferae, and S. olivieri, have all been reported to be serious pests of mango fruits. Morphology, biology, and various management approaches of these economically important weevils have been well studied. However, no mitochondrial genomes have been reported from the genus Sternochetus. Herein, we assembled mitogenomes of all the three Sternochetus species to reveal their mitogenomic characteristics. A DNA library of 350 bp insert size was constructed and sequenced in Illumina's HiSeq 6000 platform with a pair-end 150 bp sequencing strategy by Novogene. The sequence reads were assembled using GetOrganelle v1.7.1 and the genes were annotated by Geneious Prime 2021.0.3 and MITOS Web Server. Coupled with 61 published mitogenomes from 13 subfamilies of Curculionidae, we reconstructed phylogenetic trees to resolve evolutionary relationships of these closely related species and also examined subfamily-level classification among Curculionidae. All three mitogenomes are doublestranded circular molecules with 22 transfer RNA genes, 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 1 noncoding control region as in other insects. Higher interspecific nucleotide divergence (about 10%) of 13

Fine-Scale Genetic Structure of Curculio chinensis (Coleoptera: Curculionidae) Based on Mitochondrial COI: The Role of Host Specificity and Spatial Distance

“…= 12, p < 0.001; C/E: t-test: t = −36.402, d.f. = 25, p < 0.001) (Figure 4) [41]. The genetic distance between the two haplogroups of C. chinensis was greater than the intraspecific distance of other species in Jiangxi.…”

Evolutionary analysis and biological characterization of a novel alphabaculovirus isolated from Mythimna separata

“…The collinearity of MbMNPV-CHN1 and the other 12 Mb-McNPV group isolates was analysed using Mauve2.4.0 in the JAVA environment [37]. Geneious Prime 2021 [38] was used to calculate the number of single nucleotide variants (SNVs) between each isolate, and the numbers were converted to log values before creating a heatmap. Using MbMNPV-CHN1 as a reference, the homologous protein sequences were compared by ncbi-blast-2.11.0 (https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/) to obtain the identity values, and the rank sum values were tested using the wilcox.test function in R language [39].…”

Comparative mitogenomic and evolutionary analysis of Lycaenidae (Insecta: Lepidoptera): Potential association with high-altitude adaptation

“…In addition, animal mitogenomes usually contain a large non-coding region, known as the control region (CR) or AT-rich region in arthropods, which contains essential regulatory elements for transcription and replication ( Boore, 1999 ; Zardoya and Suadrez, 2008 ). Mitogenomes have been used in population genetics, phylogeography, and phylogenetic studies of various taxa, e.g., insects ( Yuan et al, 2015 ; Liu et al, 2022a ; Zhang et al, 2022 ), spiders ( Tyagi et al, 2020 ; Li et al, 2021 ; Li et al, 2022 ), and centipedes ( Hu et al, 2020 ; Ding et al, 2022 ). To date, only nine sequenced mitogenomes of nine Lycaenidae species have been stored in GenBank, which is extremely limited given the species richness, restricting our understanding of the phylogeny and evolution of the family.…”