Mitochondrial genotyping of an endangered bitterling Acheilognathus typus (Cyprinidae)

Saitoh, Kenji; Shindo, Kentaro; Fujimoto, Yasufumi; Takahashi, Kyoko; Shimada, Takehiko

doi:10.3897/zookeys.623.8981

Cited by 7 publications

(8 citation statements)

References 12 publications

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“…Three specimens of the five (1901, 1902 and 1905) carried Hap05 (haplotype 5) that is specific to a pond in Kashimadai, Miyagi [13] (Table 1). The other two (1903 and 1904) carried Hap01 that is widely distributed over northern Honshu.…”

Section: Results and Remarksmentioning

confidence: 99%

“…DNAs were extracted from fin clips with the QuickGene DNA Tissue kit and QuickGene-810 (Kurabo, Neyagawa, Japan). Primer sequences [13] and PCR/sequencing conditions [5] followed previous reports. We determined sequences of 503 bp at positions 15,640-16,142 of the complete mtDNA sequence (AB239602) [17].…”

Section: Methodsmentioning

confidence: 99%

“…An endangered bitterling, Acheilognathus typus, is endemic to the northern part of Honshu Island (Tohoku region), Japan (Figure 1) and listed in the Japan red list as critically endangered [11]. Only a few remnant populations are currently known [12,13] after a drastic decline decades ago [14] and due to invasive largemouth bass predation [15]. It is believed extinct in the Niigata prefecture [16].…”

Section: Introductionmentioning

confidence: 99%

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Unauthorized Stocking of an Endangered Bitterling Acheilognathus typus in an Irrigation Pond Detected and Substantiated by Biological and Human Lines of Evidence

Saitoh

Inoue²,

Hasegawa³

2022

Fishes

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Acheilognathus typus, an endangered bitterling, was captured in an irrigation pond in the northern part of Niigata prefecture, Japan, in 2019. This bitterling species had once occupied that region. Its absence for years indicated the possible extinction of the bitterling there. We expected that the recently captured individuals are an unknown remnant stock of that endangered species found through an extensive survey. Mitochondrial genotyping, however, revealed that the recently captured individuals had a common haplotype with those from Kashimadai, Miyagi prefecture, Japan. The uniqueness of the haplotype to these two ponds indicates a stock identity between them. In the pond in Kashimadai, the illegal activity of catching the bitterlings by a person from the northern Niigata prefecture was detected in 2015. We conclude that the bitterling from the northern Niigata prefecture was stocked from a pond in Kashimadai. Our report is the first example of unauthorized bitterling stocking substantiated from both biological and human lines of evidence.

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Section: Results and Remarksmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Unauthorized Stocking of an Endangered Bitterling Acheilognathus typus in an Irrigation Pond Detected and Substantiated by Biological and Human Lines of Evidence

Saitoh

Inoue²,

Hasegawa³

2022

Fishes

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“…Because bitterling fishes have attractive coloration to humans, there have been reports on unauthorized intentional releasing activities possibly implicated in home aquaria (Miyake et al 2011, Kitazima et al 2015, Saitoh et al 2016. Under an assumption of unauthorized releasing or dumping of P. tanago, we confront difficult issues.…”

Section: Implication Of Genetic Characteristics Of Newly Found Stocksmentioning

confidence: 99%

Natural habitats uncovered? – Genetic structure of known and newly found localities of the endangered bitterling Pseudorhodeus tanago (Cyprinidae)

Saitoh¹,

Suzuki²,

Ozaki³

et al. 2017

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Overuse of natural resources by humans is a major threat to biodiversity. Overuse often involves species of economic or esthetic value, and fish are a typical example for a group that is exploited both for economic reasons (for human consumption) and for esthetic reasons (e.g. by aquarists). Pseudorhodeus tanago (Tanaka, 1909) (formerly known as Tanakia tanago) is a small colorful but legally protected (fishing, keeping and transfer are banned) bitterling fish distributed around Tokyo, Japan. Whereas it is critically endangered and more and more habitat loss has occurred, at least four stocks have been newly found during the last decade. To explore whether emergence of these newly found habitats is a consequence of incomplete survey, we genotyped mitochondrial cytochrome b sequence of P. tanago from 17 localities and an illegal home aquarium. Populations known by the past extensive survey (13 localities) showed geographically structured population genetic characteristics. Population-specific haplotypes were common indicating past divergence and bottleneck events. Four (north, {center + west}, south_1, south_2) or five RESEARCH ARTICLE Launched to accelerate biodiversity conservation A peer-reviewed open-access journalKenji Saitoh et al. / Nature Conservation 17: 19-33 (2017) 20 (north, center, west, south_1, south_2) geographic groups were detectable as for these known localities. On the other hand, newly found stocks were polymorphic and showed identical haplotypes from distant known localities. If we assume historical basis of distribution and genetic characteristics of these newly found stocks, it must be a series of unlikely geological events and haplotype sorting. We discuss potential issues posed by these questionable stocks.

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“…However, almost all species of Japanese bitterling species are at a risk of extinction due to the deteriorating river environment and the invasion of predatory alien fish such as largemouth bass, Micropterus salmoides and the competitive alien bitterling, Rhodeus ocellatus ocellatus and Acheilognathus macropterus. The phylogeny and phylogeography of the Japanese bitterling is relatively well studied (Miyake et al 2011;Kitamura et al 2012;Chang et al 2014;Saitoh et al 2016;Nagata et al 2018). Recently, environmental DNA has been described useful in bitterling conservation studies (Sakata et al 2017), and mitochondrial information has become important.…”

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confidence: 99%

The complete mitochondrial genomes of two endangered bitterlingAcheilognathus tabira tohokuensisandA. tabira erythropterus(Cyprinidae, Acheilognathinae)

Nagata

Kitamura

2019

Mitochondrial DNA Part B

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Acheilognathus tabira (tabira bitterling) comprises of 5 subspecies, all of which are endangered. In this study, the mitochondrial genome (mitogenome) of the 2 subspecies, A. tabira tohokuensis and A. tabira erythropterus, whose mitogenomes have not been reported previously, was determined. The total lengths of A. tabira tohokuensis and A. tabira erythropterus mitogenomes were 16,774 bp and 16,770 bp, respectively, and were noted as slightly AT-rich. Phylogenetic analysis revealed that these 2 subspecies of A. tabira were the most closely related, out of the 5 subspecies. The deciphered mitogenomes would be useful for conservation and evolutionary studies.

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Mitochondrial genotyping of an endangered bitterling Acheilognathus typus (Cyprinidae)

Cited by 7 publications

References 12 publications

Unauthorized Stocking of an Endangered Bitterling Acheilognathus typus in an Irrigation Pond Detected and Substantiated by Biological and Human Lines of Evidence

Unauthorized Stocking of an Endangered Bitterling Acheilognathus typus in an Irrigation Pond Detected and Substantiated by Biological and Human Lines of Evidence

Natural habitats uncovered? – Genetic structure of known and newly found localities of the endangered bitterling Pseudorhodeus tanago (Cyprinidae)

The complete mitochondrial genomes of two endangered bitterlingAcheilognathus tabira tohokuensisandA. tabira erythropterus(Cyprinidae, Acheilognathinae)

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