Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing

Willenborg, Sebastian; Sanin, David E.; Jais, Alexander; Ding, Xiaolei; Ulas, Thomas; Nüchel, Julian; Popović, Milenko; MacVicar, Thomas; Langer, Thomas; Schultze, Joachim L.; Gerbaulet, Alexander; Roers, Axel; Pearce, Edward J.; Brüning, Jens C.; Trifunović, Aleksandra; Eming, Sabine A.

doi:10.1016/j.cmet.2021.10.004

Cited by 150 publications

(139 citation statements)

References 72 publications

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“… Note: Below, we describe two protocols for library preparation. The first protocol, described in step 71, uses a library preparation kit that is currently not manufactured, but we opt for detailing this workflow because it was used in the study by Willenborg et al. (2021) .…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…This is advantageous not only because it allows for the detection of a higher number of genes, but also for downstream quantitative and qualitative analysis, giving deeper insights into the heterogeneity of expression both within and between cells. Details of scRNA-seq analysis can be found in our recent publication ( Willenborg et al., 2021 ).…”

Section: Expected Outcomesmentioning

confidence: 99%

“…This study did not generate datasets. The single-cell RNA sequencing dataset mentioned in this protocol has been described in the study by Willenborg et al. (2021) .…”

Section: Data and Code Availabilitymentioning

confidence: 99%

“…This protocol is useful for analyzing the pronounced heterogeneity of activation phenotypes in wound macrophages and might be adapted to other experimental models of skin inflammation. For complete details on the use and execution of this protocol, please refer to Willenborg et al. (2021) .…”

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Willenborg et al. (2021) .…”

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confidence: 99%

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Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

et al. 2022

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Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Expected Outcomesmentioning

confidence: 99%

“…This study did not generate datasets. The single-cell RNA sequencing dataset mentioned in this protocol has been described in the study by Willenborg et al. (2021) .…”

Section: Data and Code Availabilitymentioning

confidence: 99%

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Willenborg et al. (2021) .…”

mentioning

confidence: 99%

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Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

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Immunomodulatory Hydrogels: Advanced Regenerative Tools for Diabetic Foot Ulcer

Xiong

Qian

Lü

et al. 2023

Adv Funct Materials

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Diabetic foot ulcer (DFU) is one of the most common complications of diabetes, bringing physical and mental challenges for patients due to the lack of efficient curative therapy. Despite considerable advances in pharmacological and surgical approaches, clinical trials for DFU patients remain disappointing due to the local overactive and excessive inflammation. Immunomodulatory hydrogels has significant advantages to overcome the clinical challenge of DFUs therapy. Here, recent fabrication and regenerative advances in the utilization of functional hydrogels for altering the immune microenvironment of DFUs are comprehensively reviewed. The pathological features and the healing processes of DFUs, followed by summarizing the physicochemical properties essential for the design of regenerative hydrogels for immunomodulation in DFUs, are briefly introduced. Then, the potential immuno-therapeutic modalities of hydrogels and emerging trends used to treat DFUs via multitherapeutic approaches and enhanced efficacy and safety are discussed. Taken together, by linking the structural properties of hydrogels to their functions in DFU therapy with a particular focus on immunomodulatory stimuli, this review can promote further advances in designing advanced hydrogels for DFUs, resulting in improved diabetic wound repair through translation into clinical setting in the near future.

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Acupoint Therapy with Injectable, Pro‐Healing Stem Cell‐Loaded Hydrogel Microspheres Promotes Skin Wound Healing

Yan,

Chen,

Zheng

et al. 2024

Adv Funct Materials

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Wound repair is a complex and dynamic process, involving stages of hemostasis, inflammation, proliferation, and remodeling. Standard methods currently used for clinical treatment of skin defects remain unsatisfying and alternative treatments have emerged including acupuncture mostly relying on the conventional needle method. It is applied sustained acupuncture therapy with bioengineered stem cell‐loaded hydrogel microspheres (A‐SGC), where the data gathered through transcriptomics, metabolomics, and single‐cell omics analyses are used to construct a comprehensive multi‐omics map underlying the wound regeneration process with such a therapy. Under the treatment of A‐SGC, the interactions and Yin‐Yang balance among fibroblasts, macrophages, endothelial cells, and keratinocytes facilitated skin regeneration instead of scarred healing. The genes related to the promotion of matrix synthesis and re‐epithelialization played important roles in the transition from the inflammatory granulation phase to the stromal remodeling phase. This convergence use of hydrogel microspheres, stem cells, and sustained acupuncture therapy could have previously unattainable therapeutic implications for improved wound treatment.

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Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing

Cited by 150 publications

References 72 publications

Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

Immunomodulatory Hydrogels: Advanced Regenerative Tools for Diabetic Foot Ulcer

Acupoint Therapy with Injectable, Pro‐Healing Stem Cell‐Loaded Hydrogel Microspheres Promotes Skin Wound Healing

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