1964
DOI: 10.1016/s0031-9422(00)88038-3
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Mitochondrial preparations from the fruit of the apple—I.

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1965
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Cited by 72 publications
(20 citation statements)
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“…Preparation of mitochondrial fraction. The procedure used was that described by Hulme et al (1963Hulme et al ( , 1964. This fraction will hereafter be called 'the mitochondria' (see Hulme et al, 1963).…”
Section: Methodsmentioning
confidence: 99%
“…Preparation of mitochondrial fraction. The procedure used was that described by Hulme et al (1963Hulme et al ( , 1964. This fraction will hereafter be called 'the mitochondria' (see Hulme et al, 1963).…”
Section: Methodsmentioning
confidence: 99%
“…The activity of mitochondria isolated from shoots of 2 day darkgrown seedlings utilizing different substrates is shown in Figure 1. The mitochondria appear to be in good condition, as indicated by relatively high rates of state 3 (ADP-stimulated) respiration, good respiratory control values (6)(7)(8), and several cycles of controlled respiration for oxidation of both aketoglutarate and malate. ADP:O ratios of approximately 3.0 and 2.3 for a-ketoglutarate and L-malate, respectively, indicate a relatively high efficiency of oxidative phosphorylation.…”
Section: Resultsmentioning
confidence: 99%
“…Previous reports (8)(9)(10)20) have indicated that respiratory properties of mitochondria are greatly influenced by the method of isolation and by the presence of inhibitory substances. In the current study, three methods for the isolation of mitochondria were examined.…”
Section: Discussionmentioning
confidence: 99%
“…27,40), cysteine is often added to the grinding medium (5, 6, 13-16, 24-26, 28-30, 33-36, 40, 41). Several workers, on the other hand, do not employ cysteine in their isolation media atall (17,22,31,32,39,45).…”
Section: Discussionmentioning
confidence: 99%