Mitochondrial Respiratory Electron Carriers Are Involved in Oxidative Stress during Heat Stress in <i>Saccharomyces cerevisiae</i>

Davidson, J. F.; Schiestl, Robert H.

doi:10.1128/mcb.21.24.8483-8489.2001

Cited by 207 publications

(133 citation statements)

References 32 publications

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“…We observed the largest amount of phenotypic variance when cells were grown under high temperature stress (37°) and that mt-n epistasis explained a significant component of this phenotypic variance (Figure 4). In yeast, high temperature increases oxidative stress produced by the electron transport chain (Davidson and Schiestl 2001). We found that endogenous levels of ROS were influenced by mt-n interactions for a number of strains at high temperature ( Figure 7).…”

Section: Intraspecific Mt-n Epistasismentioning

confidence: 86%

Mitochondrial-Nuclear Epistasis Contributes to Phenotypic Variation and Coadaptation in Natural Isolates of Saccharomyces cerevisiae

Paliwal¹,

Fiumera²,

Fiumera

2014

Genetics

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Mitochondria are essential multifunctional organelles whose metabolic functions, biogenesis, and maintenance are controlled through genetic interactions between mitochondrial and nuclear genomes. In natural populations, mitochondrial efficiencies may be impacted by epistatic interactions between naturally segregating genome variants. The extent that mitochondrial-nuclear epistasis contributes to the phenotypic variation present in nature is unknown. We have systematically replaced mitochondrial DNAs in a collection of divergent Saccharomyces cerevisiae yeast isolates and quantified the effects on growth rates in a variety of environments. We found that mitochondrial-nuclear interactions significantly affected growth rates and explained a substantial proportion of the phenotypic variances under some environmental conditions. Naturally occurring mitochondrial-nuclear genome combinations were more likely to provide growth advantages, but genetic distance could not predict the effects of epistasis. Interruption of naturally occurring mitochondrial-nuclear genome combinations increased endogenous reactive oxygen species in several strains to levels that were not always proportional to growth rate differences. Our results demonstrate that interactions between mitochondrial and nuclear genomes generate phenotypic diversity in natural populations of yeasts and that coadaptation of intergenomic interactions likely occurs quickly within the specific niches that yeast occupy. This study reveals the importance of considering allelic interactions between mitochondrial and nuclear genomes when investigating evolutionary relationships and mapping the genetic basis underlying complex traits. M ITOCHONDRIAL energy production, which affects virtually every aspect of cellular fitness, requires the participation of two genomes. The mitochondrial genome encodes for essential components of the oxidative phosphorylation machinery and mitochondrial ribosomal RNAs and transfer RNAs. The nuclear genome encodes nearly 1000 proteins that are imported to the organelle where they compose the majority of the mitochondrial proteome. Specific interactions between components of both genomes are required at many levels, including mitochondrial DNA (mtDNA) replication, repair, and inheritance and transcription, translation, and assembly of the electron transport chain components. The respiratory complexes themselves are heterogeneous, composed of both nuclear and mitochondrially encoded proteins. Over evolutionary time, these interactions have been optimized, in part, to regulate production of the reactive oxygen species (ROS) that are the by-products of mitochondrial respiration.Allelic variation in both genomes can affect mt-n interactions and alter mitochondrial fitness. These interactions have been shown to have direct consequences on healthrelated and life-history phenotypes in several taxa. In insects such as Drosophila and Callosobruchus (seed beetle), exchanging mtDNA variants between distinct populations has led to lowered metabo...

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Section: Intraspecific Mt-n Epistasismentioning

confidence: 86%

Mitochondrial-Nuclear Epistasis Contributes to Phenotypic Variation and Coadaptation in Natural Isolates of Saccharomyces cerevisiae

Paliwal¹,

Fiumera²,

Fiumera

2014

Genetics

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“…Trichophyton sp. was rich in mitochondria which was a target of oxidative stress 21 . Heat-stressed cells have been reported to exhibit increased sensitivity to low levels of chemicals including essential oils 22 .…”

Section: Discussionmentioning

confidence: 99%

Combined Effect of Heat, Essential Oils and Salt on the Fungicidal Activity against Trichophyton mentagrophytes in Foot Bath

Inouye

Uchida²,

Nishiyama

et al. 2007

Nippon Ishinkin Gakkai Zasshi

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This work was originally undertaken to determine the effective conditions of essential oils against Trichophyton mentagrophytes in vitro for the treatment of tinea pedis in a foot bath. Agar blocks implanted with T. mentagrophytes were immersed in 0.1% aqueous agar containing two-fold dilutions of essential oils with or without sodium chloride at 27 C, 37 C and 42 C for 10 and 20 min. The number of surviving mycelia on the agar blocks was determined from the standard curves of the colony diameter and original inocula of the conidia. At the same time, the thermal effect on the cellular morphology was examined using SEM. Most fungal mycelia 99.7% were killed after treatment at 42 C for 20 min without essential oil. The fungicidal activity of essential oils was markedly enhanced by treating at 42 C for 20 min as compared with that at 27 C, showing 1/4 1/32-fold reduction of minimum fungicidal concentration MFC to kill 99.99% . The order of the fungicidal activity of 11 essential oils was oregano, thyme thymol, cinnamon bark lemongrass clove, palmarose, peppermint, lavender geranium Bourbon, tea tree thyme geraniol oils. MFCs were further reduced to 1/2 1/8 by the addition of 10% sodium chloride. The salt effect was explained, at least partly, by an increase in mycelial adsorption of antifungal constituents in the presence of sodium chloride. Considerable hyphal damage was done at 27 C by the essential oils, but no further alteration in morphology of the hyphae treated at 42 C with or without oil was observed by SEM. The inhibitory effect of heat and oils was also observed against mycelia of T. rubrum and conidia of T. mentagrophytes. Thermotherapy combined with essential oils and salt would be promising to treat tinea pedis in a foot bath.

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“…Heat-treated plants are also usually subjected to oxidative injuries as a result of an increased production of oxygen radicals in chloroplasts and mitochondria, and these organelles react by production of specific anti-stress proteins to eliminate unfavorable effects (Yordanov 1992;Davidson and Schiestl 2001;Silva et al 2010). Rizhsky et al (2002Rizhsky et al ( , 2004 reported increased levels of transcripts encoding reactive oxygen intermediate-detoxifying enzymes in the treated mitochondria.…”

mentioning

confidence: 99%

Drought, high temperature, and their combination affect ultrastructure of chloroplasts and mitochondria in wheat (Triticum aestivumL.) leaves

Grigorova

Vassileva

Климчук

et al. 2012

Journal of Plant Interactions

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Plants experience a number of limiting factors, as drought and heat, which are often coinciding stress factors in natural environment. This study evaluated the changes in mesophyll cell ultrastructure in the leaves of two varieties of winter wheat (Triticum aestivum L.), differing in their drought tolerance, under individual or combined drought and heat treatment. Although the individual stress factors affected leaf ultrastructure, the damaging effect of the combined drought and heat was more pronounced and manifested certain differences between genotypes. Chloroplasts and mitochondria were affected in a variety-specific manner under all adverse treatments. The organelles of the drought-tolerant Katya were better preserved than those in the sensitive variety Sadovo. Leaf ultrastructure can be considered as one of the important characteristics in the evaluation of the drought susceptibility of different wheat varieties.

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Mitochondrial Respiratory Electron Carriers Are Involved in Oxidative Stress during Heat Stress in Saccharomyces cerevisiae

Cited by 207 publications

References 32 publications

Mitochondrial-Nuclear Epistasis Contributes to Phenotypic Variation and Coadaptation in Natural Isolates of Saccharomyces cerevisiae

Mitochondrial-Nuclear Epistasis Contributes to Phenotypic Variation and Coadaptation in Natural Isolates of Saccharomyces cerevisiae

Combined Effect of Heat, Essential Oils and Salt on the Fungicidal Activity against Trichophyton mentagrophytes in Foot Bath

Drought, high temperature, and their combination affect ultrastructure of chloroplasts and mitochondria in wheat (Triticum aestivumL.) leaves

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