Mitogen-Activated Protein Kinase and Substrate Identification in Plant Growth and Development

Jiang, Min; Zhang, Youze; Jian, Jinjing; Geyi, Wen

doi:10.3390/ijms23052744

Cited by 31 publications

(16 citation statements)

References 138 publications

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“…The results above suggest that plant hormone pathways were significantly affected by C. fulvum infection. More importantly, many pathways associated with plant disease resistance were activated, such as Plant-pathogen interaction (ko04626), MAPK signaling pathway – plant (ko04016), and Peroxisome (ko04146) ( Nyathi and Baker, 2006 ; Jiang et al., 2022a ). In addition, many metabolic pathways such as Starch and sucrose metabolism (ko00500) and Circadian rhythm – plant (ko04712) were also altered, indicating that pathogenic fungal infection can also affect normal plant growth and development.…”

Section: Resultsmentioning

confidence: 99%

Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

Peng

Sun

et al. 2022

Front. Plant Sci.

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Tomato leaf mold caused by Cladosporium fulvum (C. fulvum) is a serious fungal disease which results in huge yield losses in tomato cultivation worldwide. In our study, we discovered that ROS (reactive oxygen species) burst was triggered by C. fulvum treatment in tomato leaves. RNA-sequencing was used to identify differentially expressed genes (DEGs) induced by C. fulvum inoculation at the early stage of invasion in susceptible tomato plants. Gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to annotate functions of DEGs in tomato plants. Based on our comparative analysis, DEGs related to plant-pathogen interaction pathway, plant hormone signal transduction pathway and the plant phenylpropanoid pathway were further analyzed. Our results discovered that a number of core defense genes against fungal invasion were induced and plant hormone signal transduction pathways were impacted by C. fulvum inoculation. Further, our results showed that SA (salicylic acid) and ABA (abscisic acid) contents were accumulated while JA (jasmonic acid) content decreased after C. fulvum inoculation in comparison with control, and quantitative real-time PCR to detect the relative expression of genes involved in SA, ABA and JA signaling pathway further confirmed our results. Together, results will contribute to understanding the mechanisms of C. fulvum and tomato interaction in future.

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Section: Resultsmentioning

confidence: 99%

Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

Peng

Sun

et al. 2022

Front. Plant Sci.

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“…More specifically, MPK1, MPK3, MPK4, MPK9, MPK16, MAPKKK4, MAPKKK5, MAPKKK14, MAPKKK17, MAPKKK18 and MAPKKK21 exhibited differential expression patterns in WT‐GmMKK2 and/or KD‐GmMKK2 samples under infected or non‐infected conditions. This finding suggests that MAPKs form complex and highly interconnected signalling cascades, most probably due to their multifaceted nature of activation, regulation and function in various aspects of plant development as well as biotic and abiotic stresses (Bigeard & Hirt, 2018 ; Jiang et al., 2022 ). Of note is that several genes encoding MAPKKK21 and MAPK4 were downregulated in WT‐GmMKK2 but upregulated in KD‐GmMKK2 plants, implying that a feedback mechanism regulating the expression of GmMKK2 may exist in which MAPKKK21 and MAPK4 function, respectively, upstream and downstream of GmMKK2.…”

Section: Discussionmentioning

confidence: 99%

Soybean MKK2 establishes intricate signalling pathways to regulate soybean response to cyst nematode infection

Hawk,

Piya,

Sultana

et al. 2024

Molecular Plant Pathology

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Mitogen‐activated protein kinase (MPK) cascades play central signalling roles in plant immunity and stress response. The soybean orthologue of MPK kinase2 (GmMKK2) was recently identified as a potential signalling node whose expression is upregulated in the feeding site induced by soybean cyst nematode (SCN, Heterodera glycines). To investigate the role of GmMKK2 in soybean–SCN interactions, we overexpressed a catabolically inactive variant referred to as kinase‐dead variant (KD‐GmMKK2) using transgenic hairy roots. KD‐GmMKK2 overexpression caused significant reduction in soybean susceptibility to SCN, while overexpression of the wild‐type variant (WT‐GmMKK2) exhibited no effect on susceptibility. Transcriptome analysis indicated that KD‐GmMKK2 overexpressing plants are primed for SCN resistance via constitutive activation of defence signalling, particularly those related to chitin, respiratory burst, hydrogen peroxide and salicylic acid. Phosphoproteomic profiling of the WT‐GmMKK2 and KD‐GmMKK2 root samples upon SCN infection resulted in the identification of 391 potential targets of GmMKK2. These targets are involved in a broad range of biological processes, including defence signalling, vesicle fusion, chromatin remodelling and nuclear organization among others. Furthermore, GmMKK2 mediates phosphorylation of numerous transcriptional and translational regulators, pointing to the presence of signalling shortcuts besides the canonical MAPK cascades to initiate downstream signalling that eventually regulates gene expression and translation initiation. Finally, the functional requirement of specific phosphorylation sites for soybean response to SCN infection was validated by overexpressing phospho‐mimic and phospho‐dead variants of two differentially phosphorylated proteins SUN1 and IDD4. Together, our analyses identify GmMKK2 impacts on signalling modules that regulate soybean response to SCN infection.

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“…823 hypothetical TFs from 24 different families had been identi ed. The top 10 TF families included AP2/ERF-ERF (96), bHLH (74), C2H2 (58), NAC (54), B3 (44), MYB (44), MYB-related (44), bZIP (39), and M-type-MADS (37) families (Fig. 8A).…”

Section: The Analysis Of Transcription Factor and Weighted Correlatio...mentioning

confidence: 99%

Comparative physiology and transcriptome response patterns in cold-tolerant and cold-sensitive varieties of Solanum melongena

cai,

lan,

Gong

et al. 2023

Preprint

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Background Climate change has caused severe cold, affecting global crop production. Eggplant (Solanum melongena L.) is an important economic crop, whose yield and quality are easily severely affected by cold damage. Identifying key genes and comprehensive transcriptional regulation molecular mechanisms for cold resistance is essential to grow new varieties with stronger tolerance. Results To explore the response mechanism of eggplants to cold stress, this study compared the leaf physiological indexes and transcriptome sequencing results of cold-tolerant "A" and cold-sensitive "B" treated at 5 ℃ for 0, 1, 2, 4, and 7 d, respectively. The late physiological cold response of cultivar B was similar to the early physiological cold response of cultivar A through the analysis of chemical stoichiometry. The VIP values of peroxidase (POD) activity and soluble protein content are 1.09 and 1.12, respectively, using orthogonal partial least squares discriminant analysis (OPLS-DA), which are identified as important physiological indicators for the two varieties. RNA seq data analysis under low-temperature stress showed that 7024 differentially expressed genes (DEGs) were identified in A, and 6209 DEGs were identified in B. GO analysis showed that protein modification transport, membrane components, plant hormone signal transduction, photosynthesis, calcium and mitogen-activated protein kinase (MAPK) signal pathways, active oxygen scavenging, energy metabolism, and carbohydrate metabolism were closely related to the cold stress response of eggplant. The KEGG pathway enrichment of DEGs showed that starch and sucrose metabolism, GSH metabolism, terpenoid synthesis, and energy metabolism (TCA and HMP cycling) were promoted by low-temperature stress, improving antioxidant activity and stress resistance. Weighted gene co-expression network analysis (WGCNA) showed that many cold response genes, pathways, and soluble proteins were enriched in the MEgrep60 modules. The core hub genes of the co-expression network were POD, membrane transporter-related gene MDR1, abscisic acid-related genes (PP2C and SnRK2), growth factor enrichment gene DELLA, core components of biological clock PRR7 and five transcription factors (MYB, AP2/ERF, bZIP, bHLH, C2H2), respectively. The core transcription factor MYB was co-expressed with signal transduction, plant hormone, biosynthesis, and metabolism-related genes, indicating that this transcription factor played a key role in the cold response network. Conclusion This study integrates physiological indicators and transcriptomics to reveal the molecular mechanisms underlying the differences in cold tolerance between eggplant cold tolerant variety “A” and cold sensitive variety “B”, including ROS modulation (glutathione), increase in the content of osmotic carbohydrate and free proline, and the expression of terpenoids synthesis genes, which will help to reveal how key cold responsive transcription factors or other related genes are involved in through network. It also provides new insights into the molecular mechanisms underlying cold stress tolerance and helping to improve crop cold tolerance.

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Mitogen-Activated Protein Kinase and Substrate Identification in Plant Growth and Development

Cited by 31 publications

References 138 publications

Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

Soybean MKK2 establishes intricate signalling pathways to regulate soybean response to cyst nematode infection

Comparative physiology and transcriptome response patterns in cold-tolerant and cold-sensitive varieties of Solanum melongena

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