Mitogenic and Chemotactic Responses of Human Periodontal Ligament Cells to the Different Isoforms of Platelet-derived Growth Factor

Liu, Boyan; Bhargava, Girija; Nishimura, Fusanori; Orman, Rena; Price, Ryan; Terranova, Victor P.

doi:10.1177/00220345940730100301

Cited by 79 publications

(77 citation statements)

References 54 publications

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“…All of these participate in the wound-healing process, but, until now, only the three isoforms PDGF-AA, BB and AB were evaluated in periodontal therapy. PDGF-BB is the most effective on PDL cell mitogenesis and matrix biosynthesis [75,76]. Several preclinical studies were performed using the combination of PDGF-BB and IGF-I for periodontal and peri-implant bone regeneration [77][78][79][80][81][82], culminating in the first study in humans using growth factors for periodontal regeneration [83].…”

Section: Growth Factor Applications For Oral and Periodontal Tissue Ementioning

confidence: 99%

Growth factor delivery for oral and periodontal tissue engineering

Kaigler

Cirelli

Giannobile

2006

Expert Opinion on Drug Delivery

169

153

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The treatment of oral and periodontal diseases and associated anomalies accounts for a significant proportion of the healthcare burden, with the manifestations of these conditions being functionally and psychologically debilitating. Growth factors are critical to the development, maturation, maintenance and repair of craniofacial tissues, as they establish an extracellular environment that is conducive to cell and tissue growth. Tissue-engineering principles aim to exploit these properties in the development of biomimetic materials that can provide an appropriate microenvironment for tissue development. These materials have been constructed into devices that can be used as vehicles for delivery of cells, growth factors and DNA. In this review, different mechanisms of drug delivery are addressed in the context of novel approaches to reconstruct and engineer oral-and tooth-supporting structures, namely the periodontium and alveolar bone.

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Section: Growth Factor Applications For Oral and Periodontal Tissue Ementioning

confidence: 99%

Growth factor delivery for oral and periodontal tissue engineering

Kaigler

Cirelli

Giannobile

2006

Expert Opinion on Drug Delivery

169

153

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“…5 The mean values and standard deviations of both cell count and cell shape were then calculated per each group. One way analysis of variance (ANOVA) using Scheffee test was used for multiple comparisons among different experimental groups, and paired t test was used to compare between the different incubation time periods within each experimental group.…”

Section: Discussionmentioning

confidence: 99%

“…We selected the use of BB homodimer of PDGF since the cellular response of PDL fibroblasts to PDGF depend on the expression of a and b PDGF receptors, which are protein tyrosine kinase receptors as suggested by Heldin et al [41]. Accordingly, Boyan et al [5] showed that the chemotaxis and mitogenesis responses of human PDL fibroblast are strongest to the BB homodimer of PDGF that binds both of these receptors.…”

Section: Discussionmentioning

confidence: 99%

“…Platelet-derived growth factor (PDGF) is extensively used both in vitro and in vivo oral wound healing models, either alone [4][5][6][7] or in combination with other agents such as insulin-like growth factor-1 (IGF-1) [8,9], extracellular matrix molecules [10], lysophosphatidic acid [11] and/or with other different agents [12]. PDGF influences migration, proliferation and synthetic activity of periodontal ligament (PDL) fibroblast cells [5,13,14]. In our previous reports, we found that PDGF showed a positive effect on adhesion and growth of cultures fibroblasts to periodontally diseased surfaces [15].…”

Section: Introductionmentioning

confidence: 99%

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Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface

et al. 2012

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Periodontal regeneration using EDTA or PDGF showed promising results, but the effect of combined application was still unclear. This study aimed to verify the effect of EDTA and/or PDGF application on root adhesion and proliferation of PDL fibroblast cells. Eighty specimens were prepared from forty periodontitis teeth and made five groups: (1) diseased (untreated), (2) SRP (scaling root planing), (3) EDTA (24%), (4) PDGF (25 ng/ml) and (5) Combined application of EDTA and PDGF. Periodontal ligament cells were cultured on the above conditioned dentin plate, and SEM examination was preformed and cells were counted within a representative standard area for both cell morphology and density. All groups including untreated showed significantly increase of adhered cells from baseline to 7 days. Among them, rate of increase was much higher in EDTA, PDGF, and combined groups. ANOVA test indicated that the number of cells in PDGF and combined groups was significantly higher than diseased group at 1 day. On day 7, PDGF and combined groups showed significantly higher number of adhesion cells than that found in the diseased, SRP and EDTA groups. Thus, root conditioning with EDTA enhanced cell adhesion more than SRP alone. There was no significant difference of cell number between PDGF and combined group. Combined application of EDTA and PDGF increased significantly PDL cell adhesion than EDTA alone. PDGF alone, however, also showed comparable effect to combined application at all periods. Thus, synergistic effect between PDGF and EDTA was not observed.

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“…PDL cells were grown in 150-cm2 flasks in 25 mL of normal-glucose DMEM supplemented with 10°FBS. When the cells reached subconfluence, the medium was changed to Minimum Essential Medium (MEM, pH 7.4; GIBCO) supplemented with 50 mg/L of ascorbic acid, and the cells were incubated for 48 hr so that cell cycles would be synchronized (Boyan et al, 1994). At various time points (up to 48 hr) after serum re-stimulation (with normal-glucose DMEM containing 10% FBS), the cells were trypsinized and harvested, followed by digestion of cellular protein and RNA.…”

Section: Culture Conditionsmentioning

confidence: 99%

Glucose-mediated Alteration of Cellular Function in Human Periodontal Ligament Cells

et al. 1996

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Because diabetic patients are easily led to manifest severe periodontitis, we wanted to determine whether various glucose levels interfere with normal cellular function. Human periodontal ligament (PDL) cells were cultured in glucose-free medium, or in medium containing either 1100 mg/L of glucose (normal-glucose medium) or 4500 mg/L of glucose (high-glucose medium). Cells cultured in glucose-free medium changed their morphology from spindle-shaped to round, and incorporated trypan blue in a time-dependent manner. The incorporation rate was much faster in cells with shorter cell cycles than in those with longer cycles, suggesting the involvement of cell-cycle progression in cell death. However, fragmented DNA, which suggests apoptotic cell death, was not observed in these cells. We reasoned that initial cell rounding and detachment from the culture plate might be due to the conformational changes in cell-surface receptors to fibronectin, a major extracellular matrix for fibroblasts. Western blot analysis revealed that cells cultured in glucose-free medium lost their fibronectin receptor in a time-dependent manner. In addition, fibronectin receptor expression was much higher in cells cultured in high-glucose medium than in cells cultured in normal-glucose medium. Furthermore, the over-expression of the fibronectin receptor resulted in a suppressed chemotactic response of these cells to platelet-derived growth factor. On the basis of these data, it was hypothesized that a high glucose level induced over-expression of these receptors. This might be the mechanism by which a high glucose level compromises wound healing in diabetic patients and, at least in part, might be the reason diabetic patients are subject to severe periodontal destruction.

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Mitogenic and Chemotactic Responses of Human Periodontal Ligament Cells to the Different Isoforms of Platelet-derived Growth Factor

Cited by 79 publications

References 54 publications

Growth factor delivery for oral and periodontal tissue engineering

Growth factor delivery for oral and periodontal tissue engineering

Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface

Glucose-mediated Alteration of Cellular Function in Human Periodontal Ligament Cells

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