Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface

Belal, Mahmoud Helmy; Watanabe, Hisashi; Ichinose, Shizuko; Ishikawa, Isao

doi:10.1007/s10266-011-0046-x

Cited by 10 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A recent in vitro study demonstrated a higher rate of periodontal cell adhesion when using EDTA, PDGF, or a combination of both, compared to SRP (scaling and root-planing) or untreated groups. However, there was no significant difference between PDGF and PDGF + EDTA groups [76]. Other dimers such as PDGF-AB have also been tested, but clinical application has not yet been approved [77].…”

Section: Platelet-derived Growth Factormentioning

confidence: 99%

Stem Cells, Scaffolds and Gene Therapy for Periodontal Engineering

Padial‐Molina

Ríos

2013

Curr Oral Health Rep

View full text Add to dashboard Cite

The periodontium is continually exposed to a variety of challenges. Over the past decade, preclinical and clinical research has highlighted the complex modulatory role of the periodontal ligament (PDL) as a mediator of tissue repair and homeostasis. Experiments carried out in both human and animal models have highlighted the importance of the PDL as a protective structure. The unique properties of PDL cells endow this tissue with functional attributes that are not replicated by other biological systems. Furthermore, distinct PDL matricellular properties favor a synchronized molecular response to environmental challenges that supports normal dental and alveolar adaptation. Today, the mechanism by which periodontal integrity is restored and maintained is the focus of novel and innovative research. The ability to decipher the molecular mechanisms that support periodontal homeostasis, together with emerging science in biomaterials and stem cell biology, represents a unique opportunity to enhance the predictability of current regenerative surgical approaches and to develop novel treatment strategies for periodontal tissue engineering. This review will focus on the most recent available information concerning cells, gene delivery, and new scaffold technologies that are relevant in periodontal regeneration.

show abstract

Section: Platelet-derived Growth Factormentioning

confidence: 99%

Stem Cells, Scaffolds and Gene Therapy for Periodontal Engineering

Padial‐Molina

Ríos

2013

Curr Oral Health Rep

View full text Add to dashboard Cite

show abstract

“…In fact, there are products currently available commercially for applications in periodontal regeneration based on the biological properties of growth factors. One such product includes in its surgical procedure protocol acid conditioning of the root surface to remove smear layer from the dentin 4 and there is evidence indicating that chemical conditioning with EDTA improved PDGF-induced adhesion of periodontal ligament fibroblasts in comparison with the use of PDGF without previous EDTA conditioning 3 .…”

Section: Introductionmentioning

confidence: 99%

Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration

et al. 2017

View full text Add to dashboard Cite

Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration.Material and Methods:Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibroblast growth factor (bFGF, 10 and 50 ng). We used ELISA to assess the dynamics of bFGF release from the dentin surface and RT-qPCR to study the expression of Runx2, Col1a1, Bglap and fibronectin by periodontal ligament (PDL) fibroblasts, cementoblasts and bone marrow stromal cells (BMSC) grown onto these dentin slices. We also assessed the effects of topical application of bFGF on cell proliferation by quantification of genomic DNA.Results:Acid conditioning significantly increased the release of bFGF from dentin slices. Overall, bFGF application significantly (p<0.05) increased cell proliferation, except for BMSC grown on non-conditioned dentin slices. Dentin substrate discretely increased expression of Col1a1 in all cell types. Expression of Runx2, Col1a1 and Fn was either unaffected or inhibited by bFGF application in all cell types. We could not detect expression of the target genes on BMSC grown onto conditioned dentin.Conclusion:Acid conditioning of dentin improves the release of topically-applied bFGF. Topical application of bFGF had a stimulatory effect on proliferation of PDL fibroblasts, cementoblasts and BMSC, but did not affect expression of Runx2, Col1a1, Bglap and fibronectin by these cells.

show abstract

“…However, conflicting results regarding the etching effect of these materials have been reported depending on their treatment modalities (Mariotti, ). Platelet‐derived growth factor is a potent stimulator on adhesion and growth of cultured periodontal ligament cells (PDL) to periodontally diseased surfaces and showed to have a promising role in clinical periodontics (Belal et al, ). Laser therapy has recently been introduced for wide range of dental applications.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of fibroblast attachment in root conditioning with Er, Cr:YSGG laser versus EDTA: A SEM study

Fekrazad

Lotfi

Harandi

et al. 2015

Microsc. Res. Tech.

View full text Add to dashboard Cite

The regeneration of periodontal support is a main concern in periodontal therapy. This study aims to investigate the efficacy of Er, Cr:YSGG laser and EDTA based conditioning in attachment of fibroblast on root surfaces. This in vitro study was conducted on 81 root plates (6 mm × 4 mm × 1 mm) prepared from 27 single-rooted human mature teeth. The samples were divided into three groups: (1) Er, Cr: YSGG laser conditioning with a G6 tip (2.78 µm, 0.75 W, pulse duration of 140 µs, repetition rate of 20 Hz) for 5-7 s; (2) EDTA conditioning (17%, pH: 8) for 1 min; and (3) the control group which were exposed neither to EDTA nor laser. The viability and proliferation rates assessments were performed using MTT assay on days 3 and 5. In addition, the level of cell attachment was studied using scanning electron microscopy. The data indicated Er, Cr:YSGG conditioning increased cell viability by lapse of time (from days 3-5), with significantly better cell attachment compared to the other groups on days 3 and 5 (P < 0.05). In addition, increasing cell attachment in the EDTA conditioning group compared with the control group was statistically significant on day 5 but not on day 3 (P < 0.05). In conclusion, Er, Cr:YSGG laser conditioning can promote enhance fibroblast attachment on dentinal root surfaces more than EDTA.

show abstract

Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface

Cited by 10 publications

References 34 publications

Stem Cells, Scaffolds and Gene Therapy for Periodontal Engineering

Stem Cells, Scaffolds and Gene Therapy for Periodontal Engineering

Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration

Evaluation of fibroblast attachment in root conditioning with Er, Cr:YSGG laser versus EDTA: A SEM study

Contact Info

Product

Resources

About