Mixed Protein Carriers for Modulating DNA Release

Morán, Carmen; Pais, Alberto A. C. C.; Ramalho, A.; Miguel, Maria G.; Lindman, Björn

doi:10.1021/la901071v

Cited by 21 publications

(33 citation statements)

References 30 publications

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“…Studies of DNA release from protein-DNA particles formed by the dropwise addition method have demonstrated that these particles can present DNA release profiles of up to 1,000 h, confirming the stability of these protein-DNA gel particles [63]. In the present study, the stability of these particles in the culture medium was also confirmed.…”

Section: Cytotoxicitysupporting

confidence: 83%

“…The development of biodegradable, biocompatible DNA gel particles has been achieved using natural proteins or mixtures of proteins [52,[63][64] Lysozyme is one of…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…Interestingly, highest LC value were obtained for particles containing the pure protein PS or mixed systems containing the smallest amount of protamine sulfate PS in the mixed protein systems [63]. These differences could be attributed to differences in the binding characteristics of these two proteins, with different total charge and linear charge density: LS is a globular protein that has a net charge of + 9 at neutral pH, whereas PS is a highly positively charged linear protein with an overall charge of +21.…”

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confidence: 99%

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DNA gel particles: An overview

Morán

Vinardell

Infante

et al. 2014

Advances in Colloid and Interface Science

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A general understanding of interactions between DNA and oppositely charged compounds forms the basis for developing novel DNA-based materials, including gel particles. The association strength, which is altered by varying the chemical structure of the cationic cosolute, determines the spatial homogeneity of the gelation process, creating DNA reservoir devices and DNA matrix devices that can be designed to release either single-(ssDNA) or double-stranded (dsDNA). This review covers recent developments on the topic of DNA gel particles formed in water-water emulsion-type interfaces. The degree of DNA entrapment, particle morphology, swelling/dissolution behaviour and DNA release responses are discussed as a function of the nature of the cationic agent used. On the basis of designing DNA gel particles for therapeutic purposes, recent studies on the determination of the surface hydrophobicity, the haemolytic and the cytotoxic assessments of the obtained DNA gel particles have been also reported.

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Section: Cytotoxicitysupporting

confidence: 83%

“…The development of biodegradable, biocompatible DNA gel particles has been achieved using natural proteins or mixtures of proteins [52,[63][64] Lysozyme is one of…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

mentioning

confidence: 99%

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DNA gel particles: An overview

Morán

Vinardell

Infante

et al. 2014

Advances in Colloid and Interface Science

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“…Each one of the release stages was related to a specific value of n and k, suggesting that the overall release profile of Vit-B 12 from the protein-based microparticles is predicted by different types of mechanisms and release rates. These two step-release mechanisms have been found for release of DNA [41] and BSA and FITC-dextran [42] from DNA-based gels. Table 2 summarizes the data of n and k and other adjusting parameters for the two stages, where subscripts 1 and 2 represent the first and second stage, respectively.…”

Section: Vit-b 12 Release From the Microparticlesmentioning

confidence: 77%

Drug release mechanisms of chemically cross-linked albumin microparticles: Effect of the matrix erosion

Sitta

Guilherme

Silva

et al. 2014

Colloids and Surfaces B: Biointerfaces

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d M a n u s c r i p t Page 4 of 25 A c c e p t e d M a n u s c r i p tsphericity. In such a case, BSA played a role as a surface active agent, replacing PVA. For longer stirring times, BSA was unable to act as an emulsifier.These microparticles showed an uncommon release profile, consisting of a two-step release mechanism, at the pH range studied. Considering that a two-step release mechanism is occurring, the experimental data were adjusted by applying modified power law and Weibull equations in order to describe release mechanism n and release rate constant k, respectively. Each one of the release stages was related to a specific value of n and k. The second stage was driven by a super case II transport mechanism, as a result of diffusion, macromolecular relaxation, and erosion. A third model, described by Hixson-Crowell, confirmed the erosion mechanism.Vit-B 12 diffusion kinetics in aqueous solutions (i.e., without the microparticles) follows a one-step process, being k dependent on the pH, confirming that the two-step release mechanism is a characteristic profile of the developed microparticles. The microparticles released only 2.70% of their initial drug load at pH 2, and 58.53% at pH 10.

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“…A c c e p t e d M a n u s c r i p t Previous work in our lab has demonstrated the potential application of these protein-DNA gel 340 particles in the controlled encapsulation and release of DNA (Morán et al, 2009a). The magnitude 341 of the DNA release was controlled and controlled release systems were achieved by changing the 342 LS/PS ratio in the protein solution where particles were formed.…”

Section: [Fig 2 Here]mentioning

confidence: 99%