Mixture design as a first step for optimization of fermentation medium for cutinase production from Colletotrichum lindemuthianum

Abstract

Cutinase enzymes from fungi have found diverse applications in industry. However, most of the available literature on cutinase production is related to the cultivation of genetically engineered bacteria or yeast cells. In the present study, we use mixture design experiments to evaluate the influence of six nutrient elements on production of cutinase from the fungus Colletotrichum lindemuthianum. The nutritional elements were starch, glucose, ammonium sulfate, yeast extract, magnesium sulfate, and potassium phosphate. In the experimental design, we imposed the constraints that exactly one factor must be omitted in each set of experiments and no factor can account for more than one third of the mixture. Thirty different sets of experiments were designed. Results obtained showed that while starch is found to have negative influence on the production of the enzyme, yeast extract and potassium phosphate have a strong positive influence. Magnesium sulfate, ammonium sulfate, and glucose have low positive influence on the enzyme production. Contour plots have also been created to obtain information concerning the interaction effects of the media components on enzyme production.

Cutinase production by Fusarium oxysporum in liquid medium using central composite design

“…In 2007, Rispoli and Shah [18] studied the production of cutinase from Collerotrichum lindemuthianum, and found that yeast extract had a strong positive influence on the yield of the process.…”

Intensification of Fructosyltransferases and Fructo-Oligosaccharides Production in Solid State Fermentation by Aspergillus awamori GHRTS

“…Sathish et al [8] and Yan et al [14] also noticed that quadratic model is best suitable model for analysis of the mixture design. The special cubic and cubic models have the higher R [11] the model which have the higher R value and lower P value are significant based on this the cubic and special cubic models were rejected and further analysis was carried out with the quadratic model only.…”

“…Mixture experiment design has been used as an alternative to factorial experiments in elucidating the effects of nutrient proportions in plant-herbivore relationships [10]. Mixture experiments have become a subject of many studies and have also been extensively applied in biotechnology, pharmaceuticals, geology, petroleum, food, and tobacco industries [8,11]. In the present study, mixture design was employed to evaluate the influence of mixed solid substrates on the FTase production by isolated Aspergillus awamori GHRTS.…”

Optimization of expression and purification of recombinant S1 domain of the porcine epidemic diarrhea virus spike (PEDV- S1) protein inEscherichia coli

“…The production of secondary metabolites in the bacterial cells are largely known to depend upon the constituents of the medium in which they are grown [47][48][49]; however, little is known about the effect of media formulations on protein folding and accumulation of soluble fractions [50].…”