Mixture effect of arsenic and fluoride at environmentally relevant concentrations in zebrafish (Danio rerio) liver: Expression pattern of Nrf2 and related xenobiotic metabolizing enzymes

Mondal, Paritosh; Shaw, Pallab; Bandyopadhyay, Asish; Bhowmik, Arpan Dey; Chakraborty, Anindita; Sudarshan, M.; Chattopadhyay, Ansuman

doi:10.1016/j.aquatox.2019.06.002

Cited by 47 publications

(24 citation statements)

References 47 publications

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“…For example, copper–zinc SOD (Cu/Zn SOD) is present in the cytosol and in intermembrane space of mitochondria whereas manganese‐SOD (Mn‐SOD) is present in the mitochondrial matrix (Bhattacharya, ). CAT and both the types of SODs are reported to be downstream targets of Nrf2 (Akino et al, ; Mondal et al, ) Heme oxygenase 1 (HO1)HO1 is a crucial enzyme that catalyzes the first and rate‐limiting step of heme catabolism leading to the oxidative cleavage of b ‐type heme and thus yielding equimolar quantities of ferrous iron, carbon monoxide, and biliverdin. In turn, biliverdin is converted by biliverdin reductase to bilirubin.…”

Section: Downstream Target Proteins Of Nrf2mentioning

confidence: 99%

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Nrf2–ARE signaling in cellular protection: Mechanism of action and the regulatory mechanisms

Shaw

Chattopadhyay

2019

Journal Cellular Physiology

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170

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Oxidative stress is the increase in cellular oxidant concentration in comparison to antioxidant titer. Toxic insults and many other diseased conditions are mediated through the formation of such condition. Once the redox equilibrium is disrupted, the cellular antioxidant system functions to bring back the cell to redox homeostasis state. The field players of the cytoprotective machinery are the xenobiotic‐metabolizing enzymes that are transcriptionally controlled by upstream regulatory pathways like the Nrf2–ARE pathway and AhR–XRE pathway. The importance of Nrf2 lies in the fact that it is activated by a variety of compounds and has a wide range of inducers including metals, organic toxicants and so forth. The present review article aims to discuss the role of Nrf2 in cellular protection and also intends to illuminate the regulatory mechanisms that control Nrf2 itself. This can add to our knowledge of how the cell reacts and survives against such stressed conditions.

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Section: Downstream Target Proteins Of Nrf2mentioning

confidence: 99%

Section: Downstream Target Proteins Of Nrf2mentioning

confidence: 99%

Nrf2–ARE signaling in cellular protection: Mechanism of action and the regulatory mechanisms

Shaw

Chattopadhyay

2019

Journal Cellular Physiology

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318

170

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“…Soluble inorganic F ingested through water and foods are absorbed in the gastrointestinal tract. When ionic F enters the gastrointestinal tract, 20%‐25% of ingested F is absorbed in the stomach as hydrogen fluoride and the remainder is absorbed in the proximal small intestine through tight junctions as well as proton‐coupled transporter and Band3 protein anion transporter (Buzalaf & Whitford, ; Mondal et al, ). After absorption, the plasma F level increased within a few minutes and circulated to the intracellular and extracellular sites of tissues.…”

Section: Absorption Distribution Excretion and Metabolism Of Fluoridementioning

confidence: 99%

“…The activity of dopamine, norepinephrine, monoamine oxidase and acetylcholinesterase also showed less alteration upon co‐exposure. Recently, we have reported that the combined effect of As and F in zebrafish liver has an antagonistic relationship on biochemical variables (Mondal et al, ), although Jhala et al () showed synergism following co‐exposure of As and F on mouse ovary.…”

Section: Effects Of Arsenic and Fluoride Co‐exposure On Biochemical Vmentioning

confidence: 99%

“…The main sign of bone toxicity was osteosclerosis, and antagonism was found in the case of combined exposure (Hong, Zeng, Zheng, & Song, ). A recent study established the effect of As‐F co‐exposure on the Nrf2‐Keap1‐ARE pathway (Mondal et al, ). In this study, they showed the individual and combined effects of As and F on the expression of Nrf2 at the transcriptional and translational level and showed its nuclear translocation.…”

Section: Effect Of Arsenic and Fluoride At The Cellular Levelmentioning

confidence: 99%

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Environmental exposure of arsenic and fluoride and their combined toxicity: A recent update

Mondal

Chattopadhyay

2019

J of Applied Toxicology

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Environmental exposure to arsenic (As) and fluoride (F) in the recent year has been increased because of excessive use of naturally contaminated ground water.Surface water is also regularly contaminated with these elements in various industrial areas. Arsenicosis and fluorosis upon individual exposure of As and F are reported in many studies. A syndrome of endemic As poisoning and fluorosis occurs during concurrent exposure of As and F. Previous reports showed synergistic, antagonistic and independent effects of these two compounds, although few recent reports also revealed antagonistic effects after co-exposure. Interaction during intestinal absorption and influence of F on As metabolism might be the cause of antagonism. The synergism/antagonism is thought to depend on the dose and duration of the co-exposure. However, the detailed mechanism is still not fully understood and needs further studies. Removal technologies of As and F from contaminated water is available but removal of such contaminants from food is yet to be developed. Antioxidants are useful to mitigate the toxic effects of As and F. This review focused on the effect of co-exposure, amelioration as well as removal techniques of As and F. K E Y W O R D Samelioration, arsenic, co-exposure, fluoride, mixture effect, removal technology

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Environmentally relevant lead alters nuclear integrity in erythrocytes and generates oxidative stress in liver of Anabas testudineus: Involvement of Nrf2‐Keap1 regulation and expression of biomarker genes

Dey,

Mondal,

Chattopadhyay

2023

J of Applied Toxicology

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Genotoxic and hepatotoxic effects of lead (Pb) on a freshwater fish, climbing perch (Anabas testudineus) were studied at an environmentally relevant concentration (43.3 ppm). The genotoxic potential of Pb was confirmed by micronucleus study, with increased frequencies of erythrocytic nuclear alterations like lobed, blebbed, notched, fragmented, and micronuclei were observed in erythrocytes in treated groups as compared to control. Inorganic Pb induces oxidative stress which is a consequence of elevated level of Reactive Oxygen Species. Hepatotoxicity was assessed both by the oxidative stress and cellular responses that emerged due to the toxic assault of Pb in the liver, the most important detoxifying organ. Upregulation of xenobiotic metabolizing enzyme like catalase was evident after 15, 30, and 90 days of exposure, and a profound effect was observed on 30th days. The level of lipid peroxidation and reduced glutathione was increased after Pb exposure. Histoarchitectural damages of liver were distinctly evident in treated fish. Western blot analysis confirmed the expressional alterations of stress‐responsive marker proteins like Nrf2, Keap1, Hsp70, and Nqo1. Pb exposure resulted in increased expression of Hsp70, Nrf2, and Nqo1, whereas Keap1 was downregulated, suggesting the involvement of Nrf2‐Keap1 regulation as a cytoprotective mechanism against Pb toxicity.

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Mixture effect of arsenic and fluoride at environmentally relevant concentrations in zebrafish (Danio rerio) liver: Expression pattern of Nrf2 and related xenobiotic metabolizing enzymes

Cited by 47 publications

References 47 publications

Nrf2–ARE signaling in cellular protection: Mechanism of action and the regulatory mechanisms

Nrf2–ARE signaling in cellular protection: Mechanism of action and the regulatory mechanisms

Environmental exposure of arsenic and fluoride and their combined toxicity: A recent update

Environmentally relevant lead alters nuclear integrity in erythrocytes and generates oxidative stress in liver of Anabas testudineus: Involvement of Nrf2‐Keap1 regulation and expression of biomarker genes

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