MKK3-MPK6-MYC2 module positively regulates ABA biosynthesis and signalling in Arabidopsis

Verma, Deepanjali; Bhagat, Prakash Kumar; Sinha, Alok Krishna

doi:10.1007/s13562-020-00621-5

Cited by 18 publications

(15 citation statements)

References 33 publications

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“…For example, MYC2 is implicated in ABA-modulated gene expression in Arabidopsis (Abe et al, 2003;Kazan and Manners, 2008). Seed germination studies in Arabidopsis showed that MYC2 plays an active role in ABA biosynthesis and the expression of responsive Frontiers in Plant Science 11 frontiersin.org genes (Verma et 2020). In this study, SlMYC2 regulated the transcription of NCED1, which is a guard cell-specific gene and encodes a rate-limiting enzyme in the ABA biosynthesis pathway in tomato (Du et al, 2014), resulting in an increase in the ABA content in plants under drought stress (Figures 5A,B, 10), which may be one reason why SlMYC2 increases stomatal closure in response to water loss.…”

Section: Discussionmentioning

confidence: 99%

SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression

Wang²,

Peng³

et al. 2022

Front. Plant Sci.

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Drought stress limits plant development and reproduction. Multiple mechanisms in plants are activated to respond to stress. The MYC2 transcription factor is a core regulator of the jasmonate (JA) pathway and plays a vital role in the crosstalk between abscisic acid (ABA) and JA. In this study, we found that SlMYC2 responded to drought stress and regulated stomatal aperture in tomato (Solanum lycopersicum). Overexpression of SlMYC2 repressed SlCHS1 expression and decreased the flavonol content, increased the reactive oxygen species (ROS) content in guard cells and promoted the accumulation of JA and ABA in leaves. Additionally, silencing the SlCHS1 gene produced a phenotype that was similar to that of the MYC2-overexpressing (MYC2-OE) strain, especially in terms of stomatal dynamics and ROS levels. Finally, we confirmed that SlMYC2 directly repressed the expression of SlCHS1. Our study revealed that SlMYC2 drove stomatal closure by modulating the accumulation of flavonol and the JA and ABA contents, helping us decipher the mechanism of stomatal movement under drought stress.

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Section: Discussionmentioning

confidence: 99%

SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression

Wang²,

Peng³

et al. 2022

Front. Plant Sci.

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“…In our study, we identified only one MKK2 ( MAPKK ) gene and it was upregulated under salt stress in G. raimondii callus, but there were two MKK2 , one MEKK1 ( MAPKKK ), and one MPK4 ( MAPK ) that were induced and upregulated under salt stress in G. sturtianum callus. MAPK is a mitogen-activated protein kinase that interacts with ABA2 and regulates ABA synthesis (Verma et al, 2020 ). In general, MAPK activates downstream elements such as transcription factors and causes related physiological and biochemical reactions in response to stress.…”

Section: Discussionmentioning

confidence: 99%

Embryogenic Calli Induction and Salt Stress Response Revealed by RNA-Seq in Diploid Wild Species Gossypium sturtianum and Gossypium raimondii

et al. 2021

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Wild cotton species can contribute to a valuable gene pool for genetic improvement, such as genes related to salt tolerance. However, reproductive isolation of different species poses an obstacle to produce hybrids through conventional breeding. Protoplast fusion technology for somatic cell hybridization provides an opportunity for genetic manipulation and targeting of agronomic traits. Transcriptome sequencing analysis of callus under salt stress is conducive to study salt tolerance genes. In this study, calli were induced to provide materials for extracting protoplasts and also for screening salt tolerance genes. Calli were successfully induced from leaves of Gossypium sturtianum (C1 genome) and hypocotyls of G. raimondii (D5 genome), and embryogenic calli of G. sturtianum and G. raimondii were induced on a differentiation medium with different concentrations of 2, 4-D, KT, and IBA, respectively. In addition, embryogenic calli were also induced successfully from G. raimondii through suspension cultivation. Transcriptome sequencing analysis was performed on the calli of G. raimondii and G. sturtianum, which were treated with 200 mM NaCl at 0, 6, 12, 24, and 48 h, and a total of 12,524 genes were detected with different expression patterns under salt stress. Functional analysis showed that 3,482 genes, which were differentially expressed in calli of G. raimondii and G. sturtianum, were associated with biological processes of nucleic acid binding, plant hormone (such as ABA) biosynthesis, and signal transduction. We demonstrated that DEGs or TFs which related to ABA metabolism were involved in the response to salt stress, including xanthoxin dehydrogenase genes (ABA2), sucrose non-fermenting 1-related protein kinases (SnRK2), NAM, ATAT1/2, and CUC2 transcription factors (NAC), and WRKY class of zinc-finger proteins (WRKY). This research has successfully induced calli from two diploid cotton species and revealed new genes responding to salt stress in callus tissue, which will lay the foundation for protoplast fusion for further understanding of salt stress responses in cotton.

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“…Estos resultados sugieren que el módulo AtMKK1-AtMPK6 afecta la expresión de genes regulados por ABA, siendo a su vez el responsable de la producción de H 2 O 2 y de la respuesta al estrés (Xing et al, 2009) (Figura 3). Control de la germinación, crecimiento de la raíz, apertura y cierre de estomas: por una parte, se demostró que el ABA también induce la expresión y la actividad del módulo AtMPKKK17/18-AtMKK3-AtMPK6 para que se fosforile el factor transcripcional MYC2, el cual, a través de ABA2 (ABA deficient 2, por sus siglas en inglés), modula la expresión de diversos genes implicados en la biosíntesis del ABA y en la inhibición del proceso de germinación de la semilla (Verma, Bhagat & Sinha, 2020) (Figura 3). Por otra parte, se demostró que una proteína de la familia de MAPKKK, denominada AtMPKKK20/AIK1 (ABA-Insensititive protein Kinase1, por sus siglas en inglés), regula las respuestas al ABA.…”

Section: áCido Abscísico (Aba)unclassified

“…Por ejemplo, el módulo AtMKK3-AtMPK6, participa en la respuesta al ataque de insectos y patógenos mediante la regulación transcripcional del gen MYC2 de una manera dependiente del JA (Takahashi et al, 2007), pero también actúa en la señalización de la luz azul mediada por el GA (De Lucas et al, 2008). Mientras que otros estudios han demostrado su participación en la biosíntesis y señalización del ABA (Verma et al, 2020).…”

Section: Perspectivas Y Conclusionesunclassified

La participación de las cinasas de proteínas activadas por mitógenos en la señalización por hormonas en Arabidopsis thaliana L.

González-Coronel

Guevara-García

2021

TIP RECQB

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Los módulos de señalización mediados por cinasas de proteína activadas por mitógenos o MAPK (Mitogen-Activated Protein Kinases, por sus siglas en inglés), participan en la transducción de distintos tipos de señales mediante la fosforilación de diversos sustratos como las enzimas, componentes estructurales, factores transcripcionales e incluso otras cinasas. Estos módulos están conformados por miembros de tres familias de proteinas diferentes, denominadas MAPK, MEK o MAPKK (MAP cinasa cinasa) y MEKK o MAPKKK (MAP cinasa cinasa cinasa), que se activan de manera secuencial por la fosforilación de residuos de aminoácidos localizados en el dominio de activación. Mediante cascadas de MAPK las células responden a distintos tipos de estrés, además, se ha demostrado que las hormonas vegetales también ejercen su efecto en los programas de crecimiento y desarrollo de las plantas a través de estas mismas vías de señalización. En este trabajo se presenta un panorama general de los diversos mecanismos de señalización por hormonas en Arabidopsis thaliana L., en los que están involucradas cascadas de señalización de MAPK.

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MKK3-MPK6-MYC2 module positively regulates ABA biosynthesis and signalling in Arabidopsis

Cited by 18 publications

References 33 publications

SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression

SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression

Embryogenic Calli Induction and Salt Stress Response Revealed by RNA-Seq in Diploid Wild Species Gossypium sturtianum and Gossypium raimondii

La participación de las cinasas de proteínas activadas por mitógenos en la señalización por hormonas en Arabidopsis thaliana L.

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