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Teaumroong, Neung; Innok, Sasidhorn; Chunleuchanon, Somporn; Boonkerd, Nantakorn

doi:10.1023/a:1016812116538

Cited by 10 publications

(2 citation statements)

References 16 publications

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“…For ARA, nitrogenase activity was detected in each broth culture from the MPN method, following Teaumroong et al (2002). For DGGE analysis, total genomic DNA from each positive MPN/ARA broth culture, which was observed for the presence of both cyanobacterial growth and acetylene reduction, was extracted according to Innok et al (2005).…”

Section: Soil Sampling and Cyanobacterial Populationmentioning

confidence: 99%

Cyanobacterial akinete induction and its application as biofertilizer for rice cultivation

et al. 2009

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Nostoc sp. VICCR1-1 was induced in order to form akinetes on the basis of nutrient modification. Phosphorus and iron were found to be the critical for akinete differentiation, especially when both elements were omitted. The number of akinete cells increased up to 20% when compared with culturing in BG11 0 medium (without N source). In addition, CaCl 2 played a role in heterocyst differentiation, and was able to induce heterocyst ranging between 30% and 46%. In order to prepare akinetes as inoculum, the dried form of akinetes was prepared by mixing it with montmorillonite clay. The inoculum with the amount of 2.8×10 6 cells m −2 was applied to rice (Oryza sativa) fields. After harvesting, the grain yields from chemical N fertilizer, vegetative cells, and akinete inoculum treatments were not significantly different. To monitor the persistence of Nostoc sp. VICCR1-1 after harvesting, the most probable number-denaturing gradient gel electrophoresis technique using 16S rRNA gene was employed. The results indicated that the remaining population is at 2.5× 10 5 and 1.62×10 6 cells m −2 in treatments supplied with vegetative cells and akinete inocula, respectively. Akinete induction might be one of the appropriate approaches for producing cyanobacterial inoculum.

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Section: Soil Sampling and Cyanobacterial Populationmentioning

confidence: 99%

Cyanobacterial akinete induction and its application as biofertilizer for rice cultivation

et al. 2009

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“…RFLP (Restriction Fragment Length Polymorphism) (Iteman et al, 2002), RAPD (Random Amplification of Polymorphic DNA) (Prabina et al, 2005;Shishir et al, 2015), STRR (Short Tandemly Repeated Repetitive) (Akoijam and Singh, 2011, Valerio et al, 2009, Wilson et al, 2000, HIP1 (Highly Iterated Palindromes) (Neilan et al, 2003, Orcutt et al, 2002, Wilson et al, 2005, Zheng et al, 2002 and ERIC (Enterobacterial Repetitive Interspersed Consensus) (Valério et al, 2005) have been attempted with an overall aim to provide better resolution among closely related species. These repetitive sequences were diagnosed in several cyanobacterial taxa, up to now broadly in heterocystous cyanobacteria (Lyra et al, 2005, Nilsson et al, 2000, Prasanna et al, 2006, Rasmussen and Svenning, 1998, Teaumroong et al, 2002, Wilson et al, 2000, Zheng et al, 1999. but also in some non-heterocystous ones (Rasmussen and Svenning, 1998).…”

Section: Introductionmentioning

confidence: 98%

Genomic Fingerprinting Using Highly Repetitive Sequences to Differentiate Close Cyanobacterial Strains

Shokraei¹,

Fahimi²,

Blanco³

et al. 2019

Microbial Bioactives

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Background: Cyanobacterial taxonomy has experimented considerable changes due to the exploration of previously uninvestigated regions as well as the introduction of molecular tools. Challenges arose when strains collected from agricultural areas, salt waters and dry limestone did not reveal remarkable morphological differences and had a high level of similarity in the phylogeny of 16S rDNA gene sequences. The aim of the present investigation was to fingerprint members of the genera Calothrix and Nostoc based on the repetitive DNA sequences, as molecular markers for the detection of phylogenetic affinities and molecular diversity. Methods: In this research, through a polyphasic approach, the differences in morphological and genotypic features of different strains were investigated. Bacteria free cyanobacterial clones were prepared followed by morphological characterization, genomic DNA extraction and PCR with 16S rRNA, ERIC, STRR1a and HIP primers. Then the phylogenetic analyses of partial 16S rRNA genes and fingerprints were performed. Results: The results showed each marker producing unique and strain-specific banding pattern, thus highlighting the efficiency of this technique in the assessment of proximity between closely related cyanobacterial strains isolated from different climatic/geographic regions and habitats. Conclusions: This case is the first documented genomic fingerprinting from seven close cyanobacterial strains in Iran.

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Bacterial diversity and community along the succession of biological soil crusts in the Gurbantunggut Desert, Northern China

Zhang

Kong

et al. 2016

J. Basic Microbiol.

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Biological soil crusts (BSCs) are common and play critical roles in semi-arid and arid ecosystems. Bacteria, as an important community in BSCs, play critical roles in biochemical processes. However, how bacterial diversity and community change in different successional stages of BSCs is still unknown. We used 454 pyrosequencing of 16S rRNA to investigate the bacterial composition and community, and the relationships between bacterial composition and environmental factors were also explored. In different successional stages of BSCs, the number of bacteria operational taxonomic units (OTUs) detected in each sample ranged from 2572 to 3157. Proteobacteria, Cyanobacteria, Bacteroidetes were dominant in BSCs, followed by Firmicutes, Acidobacteria, and Actinobacteria. At the successional stages of BSCs, bacterial communities, OTU composition and their relative abundance notably differentiated, and Cyanobacteria, especially Microcoleus vaginatus, dominated algal crust and lichen crust, and were the main C-fixing bacteria in BSCs. Proteobacteria and Bacteroidetes increased with the development of BSCs. OTUs related to Planomicrobium Chinese, Desulfobulbus sp., Desulfomicrobium sp., Arthrobacter sp., and Ahhaerbacter sp. showed higher relative abundance in bare sand than other successional stages of BSCs, while relative abundance of Sphingomonas sp. Niastella sp., Pedobacter, Candidatus solobacter, and Streptophyta increased with the development of BSCs. In successional stages of BSCs, bacterial OTUs composition demonstrated strong correlations with soil nutrients, soil salts, and soil enzymes. Additionally, variation of bacterial composition led to different ecological function. In bare sand, some species were related with mineral metabolism or promoting plant growth, and in algal crust and lichen crust, C-fixing bacteria increased and accumulated C to the desert soil. In later developed stage of BSCs, bacteria related with decomposition of organic matter, such as Sphingomonas sp. Niastella sp., Pedobacter, and Candidatus solobacter increased. Therefore, bacterial community composition and their key ecological roles shifted to the development of BSCs.

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Untitled

Cited by 10 publications

References 16 publications

Cyanobacterial akinete induction and its application as biofertilizer for rice cultivation

Cyanobacterial akinete induction and its application as biofertilizer for rice cultivation

Genomic Fingerprinting Using Highly Repetitive Sequences to Differentiate Close Cyanobacterial Strains

Bacterial diversity and community along the succession of biological soil crusts in the Gurbantunggut Desert, Northern China

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